In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells
In current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2018/5454367 |
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| author | Alona Zlatska Inna Gordiienko Roman Vasyliev Dmitriy Zubov Olga Gubar Anzhela Rodnichenko Anton Syroeshkin Igor Zlatskiy |
| author_facet | Alona Zlatska Inna Gordiienko Roman Vasyliev Dmitriy Zubov Olga Gubar Anzhela Rodnichenko Anton Syroeshkin Igor Zlatskiy |
| author_sort | Alona Zlatska |
| collection | DOAJ |
| description | In current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. The deuterated and deuterium-depleted growth media demonstrated acute and chronic cytotoxicity, respectively. The minimal migration ability was observed in deuterated medium whereas the highest migration activity was observed in the medium with the deuterium content close to natural. The cells in deuterated growth medium demonstrated decrease in metabolic activity after three days in culture. In contrast, in deuterium-depleted medium there was an increase in ADSC metabolic activity. |
| format | Article |
| id | doaj-art-02dc9dcd8ff8455287fc36ccd3df9216 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-02dc9dcd8ff8455287fc36ccd3df92162025-08-20T03:54:15ZengWileyThe Scientific World Journal2356-61401537-744X2018-01-01201810.1155/2018/54543675454367In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem CellsAlona Zlatska0Inna Gordiienko1Roman Vasyliev2Dmitriy Zubov3Olga Gubar4Anzhela Rodnichenko5Anton Syroeshkin6Igor Zlatskiy7State Institute of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, UkraineBiotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya®, Kyiv, UkraineState Institute of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, UkraineState Institute of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, UkraineBiotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya®, Kyiv, UkraineState Institute of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, UkrainePeoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow 117198, RussiaPeoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow 117198, RussiaIn current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. The deuterated and deuterium-depleted growth media demonstrated acute and chronic cytotoxicity, respectively. The minimal migration ability was observed in deuterated medium whereas the highest migration activity was observed in the medium with the deuterium content close to natural. The cells in deuterated growth medium demonstrated decrease in metabolic activity after three days in culture. In contrast, in deuterium-depleted medium there was an increase in ADSC metabolic activity.http://dx.doi.org/10.1155/2018/5454367 |
| spellingShingle | Alona Zlatska Inna Gordiienko Roman Vasyliev Dmitriy Zubov Olga Gubar Anzhela Rodnichenko Anton Syroeshkin Igor Zlatskiy In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells The Scientific World Journal |
| title | In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells |
| title_full | In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells |
| title_fullStr | In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells |
| title_full_unstemmed | In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells |
| title_short | In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells |
| title_sort | in vitro study of deuterium effect on biological properties of human cultured adipose derived stem cells |
| url | http://dx.doi.org/10.1155/2018/5454367 |
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