Aspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production
Abstract The oleaginous fungus, Aspergillus terreus when subjected to random chemical mutagenesis led to isolation of TB21 variant with improved lipid content (78.1%) as compared to wild type (49.8%). The fungal wet biomass grown on sugarcane bagasse hydrolysate (SCBH) was subjected to one-step in-s...
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2025-02-01
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| Online Access: | https://doi.org/10.1186/s13568-024-01772-7 |
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| author | Rashmi K. Bed V. Ravi Kumar Ameeta RaviKumar |
| author_facet | Rashmi K. Bed V. Ravi Kumar Ameeta RaviKumar |
| author_sort | Rashmi K. Bed |
| collection | DOAJ |
| description | Abstract The oleaginous fungus, Aspergillus terreus when subjected to random chemical mutagenesis led to isolation of TB21 variant with improved lipid content (78.1%) as compared to wild type (49.8%). The fungal wet biomass grown on sugarcane bagasse hydrolysate (SCBH) was subjected to one-step in-situ (direct) acid transesterification to optimize its conversion to biodiesel using a 2-level factorial statistical design of experiments. The process optimization revealed that wet biomass and methanol were the most significant factors and in a short reaction time period of 5 min with low methanol: wet biomass ratio (10:1) influenced FAME production Statistical optimization studies showed that TB21 exhibited a higher FAME content of 76.5 and 38.1% (w/w) from wet and dry biomass, respectively when compared to wild type (48.1 and 24.5%). FAME productivity (0.55−1 h−1) and a yield (66 gL−1) were achieved when TB21 was grown on SCBH for 120 h at 30 °C. The FAME profile from the wet biomass of TB21 grown on SCBH had desirable amounts of saturated (77.7%), monounsaturated (7.2%), and polyunsaturated (2.4%) methyl esters. Physico-chemical properties of TB21-derived biodiesel were determined, namely, density(0.88 g cm−3), kinematic viscosity (4.1 mm s−2), iodine value (96.82), cetane number (55.31), free fatty acid content (0.15%), total acid number (0.3 NaOH mg g−1), meeting international (ASTM D6751, EN 14214) and Indian (IS 15607) standards. Thus, the direct one-pot in situ transesterification reaction using wet biomass of variant TB21 strain showed improved production and quality of biodiesel with potential large scale application using the low-cost substrate (SCBH). |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-02-01 |
| publisher | SpringerOpen |
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| spelling | doaj-art-02d116e9181143e1856138f3396259a32025-02-09T12:55:47ZengSpringerOpenAMB Express2191-08552025-02-0115111510.1186/s13568-024-01772-7Aspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel productionRashmi K. Bed0V. Ravi Kumar1Ameeta RaviKumar2Department of Biotechnology, Savitribai Phule Pune UniversityChemical Engineering and Process Development Division, National Chemical LaboratoryDepartment of Biotechnology, Savitribai Phule Pune UniversityAbstract The oleaginous fungus, Aspergillus terreus when subjected to random chemical mutagenesis led to isolation of TB21 variant with improved lipid content (78.1%) as compared to wild type (49.8%). The fungal wet biomass grown on sugarcane bagasse hydrolysate (SCBH) was subjected to one-step in-situ (direct) acid transesterification to optimize its conversion to biodiesel using a 2-level factorial statistical design of experiments. The process optimization revealed that wet biomass and methanol were the most significant factors and in a short reaction time period of 5 min with low methanol: wet biomass ratio (10:1) influenced FAME production Statistical optimization studies showed that TB21 exhibited a higher FAME content of 76.5 and 38.1% (w/w) from wet and dry biomass, respectively when compared to wild type (48.1 and 24.5%). FAME productivity (0.55−1 h−1) and a yield (66 gL−1) were achieved when TB21 was grown on SCBH for 120 h at 30 °C. The FAME profile from the wet biomass of TB21 grown on SCBH had desirable amounts of saturated (77.7%), monounsaturated (7.2%), and polyunsaturated (2.4%) methyl esters. Physico-chemical properties of TB21-derived biodiesel were determined, namely, density(0.88 g cm−3), kinematic viscosity (4.1 mm s−2), iodine value (96.82), cetane number (55.31), free fatty acid content (0.15%), total acid number (0.3 NaOH mg g−1), meeting international (ASTM D6751, EN 14214) and Indian (IS 15607) standards. Thus, the direct one-pot in situ transesterification reaction using wet biomass of variant TB21 strain showed improved production and quality of biodiesel with potential large scale application using the low-cost substrate (SCBH).https://doi.org/10.1186/s13568-024-01772-7Aspergillus TerreusBiodieselIn situ transesterificationMutantStatistical optimizationWet biomass |
| spellingShingle | Rashmi K. Bed V. Ravi Kumar Ameeta RaviKumar Aspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production AMB Express Aspergillus Terreus Biodiesel In situ transesterification Mutant Statistical optimization Wet biomass |
| title | Aspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production |
| title_full | Aspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production |
| title_fullStr | Aspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production |
| title_full_unstemmed | Aspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production |
| title_short | Aspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production |
| title_sort | aspergillus terreus variant tb21 wet biomass optimized by in situ transesterification for biodiesel production |
| topic | Aspergillus Terreus Biodiesel In situ transesterification Mutant Statistical optimization Wet biomass |
| url | https://doi.org/10.1186/s13568-024-01772-7 |
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