An at-home and electro-free COVID-19 rapid test based on colorimetric RT-LAMP
Introduction: In the fight against virus-caused pandemics like COVID-19, diagnostic tests based on RT-qPCR are essential, but they are sometimes limited by their dependence on expensive, specialized equipment and skilled personnel. Consequently, an alternative nucleic acid detection technique that...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2024-08-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/17839 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850148840217247744 |
|---|---|
| author | Diem Hong Tran Hau Thi Tran Trang Nguyen Minh Pham Le Minh Bui Huong Thi Thu Phung |
| author_facet | Diem Hong Tran Hau Thi Tran Trang Nguyen Minh Pham Le Minh Bui Huong Thi Thu Phung |
| author_sort | Diem Hong Tran |
| collection | DOAJ |
| description |
Introduction: In the fight against virus-caused pandemics like COVID-19, diagnostic tests based on RT-qPCR are essential, but they are sometimes limited by their dependence on expensive, specialized equipment and skilled personnel. Consequently, an alternative nucleic acid detection technique that overcomes these restrictions, called loop-mediated isothermal amplification following reverse transcription (RT-LAMP), has been broadly investigated. Nevertheless, the developed RT-LAMP assays for SARS-CoV-2 detection still require laboratory devices and electrical power, limiting their widespread use as rapid home tests. This work developed a flexible RT-LAMP assay that gets beyond the drawbacks of the available isothermal LAMP-based SARS-CoV-2 detection, establishing a simple and effective at-home diagnostic tool for COVID-19.
Methodology: A multiplex direct RT-LAMP assay, modified from the previously developed test was applied to simultaneously identify the two genes of SARS-CoV-2. We used a colorimetric readout, lyophilized reagents, and benchmarked an electro-free and micropipette-free method that enables sensitive and specific detection of SARS-CoV-2 in home settings.
Results: Forty-one nasopharyngeal swab samples were tested using the developed home-testing RT-LAMP (HT-LAMP) assay, showing 100% agreement with the RT-qPCR results.
Conclusions: This is the first electrically independent RT-LAMP assay successfully developed for SARS-CoV-2 detection in a home setting. Our HT-LAMP assay is thus an important development for diagnosing COVID-19 or any other infectious pandemic on a population scale.
|
| format | Article |
| id | doaj-art-02ca4511fe0845f5b8b4b2f44ae5bc37 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-02ca4511fe0845f5b8b4b2f44ae5bc372025-08-20T02:27:07ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-08-01180810.3855/jidc.17839An at-home and electro-free COVID-19 rapid test based on colorimetric RT-LAMPDiem Hong Tran0Hau Thi Tran1Trang Nguyen Minh Pham2Le Minh Bui3Huong Thi Thu Phung4NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, VietnamNTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, VietnamNTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, VietnamNTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, VietnamNTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam Introduction: In the fight against virus-caused pandemics like COVID-19, diagnostic tests based on RT-qPCR are essential, but they are sometimes limited by their dependence on expensive, specialized equipment and skilled personnel. Consequently, an alternative nucleic acid detection technique that overcomes these restrictions, called loop-mediated isothermal amplification following reverse transcription (RT-LAMP), has been broadly investigated. Nevertheless, the developed RT-LAMP assays for SARS-CoV-2 detection still require laboratory devices and electrical power, limiting their widespread use as rapid home tests. This work developed a flexible RT-LAMP assay that gets beyond the drawbacks of the available isothermal LAMP-based SARS-CoV-2 detection, establishing a simple and effective at-home diagnostic tool for COVID-19. Methodology: A multiplex direct RT-LAMP assay, modified from the previously developed test was applied to simultaneously identify the two genes of SARS-CoV-2. We used a colorimetric readout, lyophilized reagents, and benchmarked an electro-free and micropipette-free method that enables sensitive and specific detection of SARS-CoV-2 in home settings. Results: Forty-one nasopharyngeal swab samples were tested using the developed home-testing RT-LAMP (HT-LAMP) assay, showing 100% agreement with the RT-qPCR results. Conclusions: This is the first electrically independent RT-LAMP assay successfully developed for SARS-CoV-2 detection in a home setting. Our HT-LAMP assay is thus an important development for diagnosing COVID-19 or any other infectious pandemic on a population scale. https://jidc.org/index.php/journal/article/view/17839COVID-19 diagnosishome-testing kitrapid testelectro-freeRT-LAMP |
| spellingShingle | Diem Hong Tran Hau Thi Tran Trang Nguyen Minh Pham Le Minh Bui Huong Thi Thu Phung An at-home and electro-free COVID-19 rapid test based on colorimetric RT-LAMP Journal of Infection in Developing Countries COVID-19 diagnosis home-testing kit rapid test electro-free RT-LAMP |
| title | An at-home and electro-free COVID-19 rapid test based on colorimetric RT-LAMP |
| title_full | An at-home and electro-free COVID-19 rapid test based on colorimetric RT-LAMP |
| title_fullStr | An at-home and electro-free COVID-19 rapid test based on colorimetric RT-LAMP |
| title_full_unstemmed | An at-home and electro-free COVID-19 rapid test based on colorimetric RT-LAMP |
| title_short | An at-home and electro-free COVID-19 rapid test based on colorimetric RT-LAMP |
| title_sort | at home and electro free covid 19 rapid test based on colorimetric rt lamp |
| topic | COVID-19 diagnosis home-testing kit rapid test electro-free RT-LAMP |
| url | https://jidc.org/index.php/journal/article/view/17839 |
| work_keys_str_mv | AT diemhongtran anathomeandelectrofreecovid19rapidtestbasedoncolorimetricrtlamp AT hauthitran anathomeandelectrofreecovid19rapidtestbasedoncolorimetricrtlamp AT trangnguyenminhpham anathomeandelectrofreecovid19rapidtestbasedoncolorimetricrtlamp AT leminhbui anathomeandelectrofreecovid19rapidtestbasedoncolorimetricrtlamp AT huongthithuphung anathomeandelectrofreecovid19rapidtestbasedoncolorimetricrtlamp AT diemhongtran athomeandelectrofreecovid19rapidtestbasedoncolorimetricrtlamp AT hauthitran athomeandelectrofreecovid19rapidtestbasedoncolorimetricrtlamp AT trangnguyenminhpham athomeandelectrofreecovid19rapidtestbasedoncolorimetricrtlamp AT leminhbui athomeandelectrofreecovid19rapidtestbasedoncolorimetricrtlamp AT huongthithuphung athomeandelectrofreecovid19rapidtestbasedoncolorimetricrtlamp |