Cut&tag: a powerful epigenetic tool for chromatin profiling
Analysis of transcription factors and chromatin modifications at the genome-wide level provides insights into gene regulatory processes, such as transcription, cell differentiation and cellular response. Chromatin immunoprecipitation is the most popular and powerful approach for mapping chromatin, a...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/15592294.2023.2293411 |
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| _version_ | 1850169068470927360 |
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| author | Zhijun Fu Sanjie Jiang Yiwen Sun Shanqiao Zheng Liang Zong Peipei Li |
| author_facet | Zhijun Fu Sanjie Jiang Yiwen Sun Shanqiao Zheng Liang Zong Peipei Li |
| author_sort | Zhijun Fu |
| collection | DOAJ |
| description | Analysis of transcription factors and chromatin modifications at the genome-wide level provides insights into gene regulatory processes, such as transcription, cell differentiation and cellular response. Chromatin immunoprecipitation is the most popular and powerful approach for mapping chromatin, and other enzyme-tethering techniques have recently become available for living cells. Among these, Cleavage Under Targets and Tagmentation (CUT&Tag) is a relatively novel chromatin profiling method that has rapidly gained popularity in the field of epigenetics since 2019. It has also been widely adapted to map chromatin modifications and TFs in different species, illustrating the association of these chromatin epitopes with various physiological and pathological processes. Scalable single-cell CUT&Tag can be combined with distinct platforms to distinguish cellular identity, epigenetic features and even spatial chromatin profiling. In addition, CUT&Tag has been developed as a strategy for joint profiling of the epigenome, transcriptome or proteome on the same sample. In this review, we will mainly consolidate the applications of CUT&Tag and its derivatives on different platforms, give a detailed explanation of the pros and cons of this technique as well as the potential development trends and applications in the future. |
| format | Article |
| id | doaj-art-02b2c60adaab4d7bac17659c1069b253 |
| institution | OA Journals |
| issn | 1559-2294 1559-2308 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj-art-02b2c60adaab4d7bac17659c1069b2532025-08-20T02:20:49ZengTaylor & Francis GroupEpigenetics1559-22941559-23082024-12-0119110.1080/15592294.2023.2293411Cut&tag: a powerful epigenetic tool for chromatin profilingZhijun Fu0Sanjie Jiang1Yiwen Sun2Shanqiao Zheng3Liang Zong4Peipei Li5BGI Tech Solutions Co, Ltd. BGI-Shenzhen, Shenzhen, ChinaBGI Tech Solutions Co, Ltd. BGI-Shenzhen, Shenzhen, ChinaBGI Tech Solutions Co, Ltd. BGI-Shenzhen, Shenzhen, ChinaBGI Tech Solutions Co, Ltd. BGI-Shenzhen, Shenzhen, ChinaBGI Tech Solutions Co, Ltd. BGI-Wuhan, Wuhan, ChinaBGI Tech Solutions Co, Ltd. BGI-Shenzhen, Shenzhen, ChinaAnalysis of transcription factors and chromatin modifications at the genome-wide level provides insights into gene regulatory processes, such as transcription, cell differentiation and cellular response. Chromatin immunoprecipitation is the most popular and powerful approach for mapping chromatin, and other enzyme-tethering techniques have recently become available for living cells. Among these, Cleavage Under Targets and Tagmentation (CUT&Tag) is a relatively novel chromatin profiling method that has rapidly gained popularity in the field of epigenetics since 2019. It has also been widely adapted to map chromatin modifications and TFs in different species, illustrating the association of these chromatin epitopes with various physiological and pathological processes. Scalable single-cell CUT&Tag can be combined with distinct platforms to distinguish cellular identity, epigenetic features and even spatial chromatin profiling. In addition, CUT&Tag has been developed as a strategy for joint profiling of the epigenome, transcriptome or proteome on the same sample. In this review, we will mainly consolidate the applications of CUT&Tag and its derivatives on different platforms, give a detailed explanation of the pros and cons of this technique as well as the potential development trends and applications in the future.https://www.tandfonline.com/doi/10.1080/15592294.2023.2293411CUT&Tagchromatin profilingepigeneticsTn5 tagmentationsingle-cell CUT&Tag |
| spellingShingle | Zhijun Fu Sanjie Jiang Yiwen Sun Shanqiao Zheng Liang Zong Peipei Li Cut&tag: a powerful epigenetic tool for chromatin profiling Epigenetics CUT&Tag chromatin profiling epigenetics Tn5 tagmentation single-cell CUT&Tag |
| title | Cut&tag: a powerful epigenetic tool for chromatin profiling |
| title_full | Cut&tag: a powerful epigenetic tool for chromatin profiling |
| title_fullStr | Cut&tag: a powerful epigenetic tool for chromatin profiling |
| title_full_unstemmed | Cut&tag: a powerful epigenetic tool for chromatin profiling |
| title_short | Cut&tag: a powerful epigenetic tool for chromatin profiling |
| title_sort | cut tag a powerful epigenetic tool for chromatin profiling |
| topic | CUT&Tag chromatin profiling epigenetics Tn5 tagmentation single-cell CUT&Tag |
| url | https://www.tandfonline.com/doi/10.1080/15592294.2023.2293411 |
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