Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom
We have provided evidence that: (a) lethality of mice to crude Bothrops venom varies according the isogenic strain (A/J > C57Bl/6 > A/Sn > BALB/c > C3H/ HePas > DBA/2 > C3H/He); (b)BALB/c mice (LD50=100.0 μg) were injected i.p. with 50 μg of venom produced IL-6, IL-10, INF-γ, TNF-α...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359890866 |
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| author | S. F. Barros I. Friedlanskaia V. L. Petricevich T. L. Kipnis |
| author_facet | S. F. Barros I. Friedlanskaia V. L. Petricevich T. L. Kipnis |
| author_sort | S. F. Barros |
| collection | DOAJ |
| description | We have provided evidence that: (a) lethality of mice to crude Bothrops venom varies according the isogenic strain (A/J > C57Bl/6 > A/Sn > BALB/c > C3H/ HePas > DBA/2 > C3H/He); (b)BALB/c mice (LD50=100.0 μg) were injected i.p. with 50 μg of venom produced IL-6, IL-10, INF-γ, TNF-α and NO in the serum. In vitro the cells from the mice injected and challenged with the venom only released IL-10 while peritoneal macrophages released IL-10, INF-γ and less amounts of IL-6; (c) establishment of local inflammation and necrosis induced by the venom, coincides with the peaks of TNF-α, IFN-γ and NO and the damage was neutralized when the venom was incubated with a monoclonal antibody against a 60 kDa haemorrhagic factor. These results suggest that susceptibility to Bothrops a trox venom is genetically dependent but MHC independent; that IL-6, IL10, TNF-α, IFN-γ and NO can be involved in the mediation of tissue damage; and that the major venom component inducers of the lesions are haemorrhagins. |
| format | Article |
| id | doaj-art-02acfa3e663b4f8f9850f3ea347ae841 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-02acfa3e663b4f8f9850f3ea347ae8412025-02-03T01:01:58ZengWileyMediators of Inflammation0962-93511466-18611998-01-017533934610.1080/09629359890866Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venomS. F. Barros0I. Friedlanskaia1V. L. Petricevich2T. L. Kipnis3Laboratório de Imunoparasitologia, SP, BrazilLaboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av Alberto Lamego 2000, Campos dos Goytacazes, RJ CEP 28015–620, BrazilLaboratório de Imunoquimica do Instituto Butantan São Paulo, SP, BrazilLaboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av Alberto Lamego 2000, Campos dos Goytacazes, RJ CEP 28015–620, BrazilWe have provided evidence that: (a) lethality of mice to crude Bothrops venom varies according the isogenic strain (A/J > C57Bl/6 > A/Sn > BALB/c > C3H/ HePas > DBA/2 > C3H/He); (b)BALB/c mice (LD50=100.0 μg) were injected i.p. with 50 μg of venom produced IL-6, IL-10, INF-γ, TNF-α and NO in the serum. In vitro the cells from the mice injected and challenged with the venom only released IL-10 while peritoneal macrophages released IL-10, INF-γ and less amounts of IL-6; (c) establishment of local inflammation and necrosis induced by the venom, coincides with the peaks of TNF-α, IFN-γ and NO and the damage was neutralized when the venom was incubated with a monoclonal antibody against a 60 kDa haemorrhagic factor. These results suggest that susceptibility to Bothrops a trox venom is genetically dependent but MHC independent; that IL-6, IL10, TNF-α, IFN-γ and NO can be involved in the mediation of tissue damage; and that the major venom component inducers of the lesions are haemorrhagins.http://dx.doi.org/10.1080/09629359890866Bothrops a trox CytokinesAnimal venomsMediators of inflammation. |
| spellingShingle | S. F. Barros I. Friedlanskaia V. L. Petricevich T. L. Kipnis Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom Mediators of Inflammation Bothrops a trox Cytokines Animal venoms Mediators of inflammation. |
| title | Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom |
| title_full | Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom |
| title_fullStr | Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom |
| title_full_unstemmed | Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom |
| title_short | Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom |
| title_sort | local inflammation lethality and cytokine release in mice injected with bothrops atrox venom |
| topic | Bothrops a trox Cytokines Animal venoms Mediators of inflammation. |
| url | http://dx.doi.org/10.1080/09629359890866 |
| work_keys_str_mv | AT sfbarros localinflammationlethalityandcytokinereleaseinmiceinjectedwithbothropsatroxvenom AT ifriedlanskaia localinflammationlethalityandcytokinereleaseinmiceinjectedwithbothropsatroxvenom AT vlpetricevich localinflammationlethalityandcytokinereleaseinmiceinjectedwithbothropsatroxvenom AT tlkipnis localinflammationlethalityandcytokinereleaseinmiceinjectedwithbothropsatroxvenom |