Uniform impact on individual megakaryocytes is essential for efficient in vitro platelet production
Abstract Different approaches are being developed to efficiently produce in vitro platelets from cultured megakaryocytes to meet the constant demand of platelet transfusion and serve for research purposes. Recent works have shown that turbulence and periodic stress can significantly enhance platelet...
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| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-79949-0 |
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| author | Andrei K. Garzon Dasgupta Anaïs Pongérard Léa Mallo Anita Eckly François Lanza Olivier Boiron Yannick Knapp Catherine Strassel |
| author_facet | Andrei K. Garzon Dasgupta Anaïs Pongérard Léa Mallo Anita Eckly François Lanza Olivier Boiron Yannick Knapp Catherine Strassel |
| author_sort | Andrei K. Garzon Dasgupta |
| collection | DOAJ |
| description | Abstract Different approaches are being developed to efficiently produce in vitro platelets from cultured megakaryocytes to meet the constant demand of platelet transfusion and serve for research purposes. Recent works have shown that turbulence and periodic stress can significantly enhance platelet yield. Here we have developed and characterized a platelet production device that takes in account these properties. This device is based on the Taylor-Couette reactor in which a suspension is confined and sheared between two concentric cylinders. We have demonstrated that such a system allows obtaining high number of in vitro platelets per megakaryocyte with native-like morphology and functional properties. Using the combination of in silico and in vitro techniques, we claimed that overall turbulent conditions are not sufficient for efficient platelet release, and highlighted the importance of the uniform impact of flow on each megakaryocyte, a property that must be taken into account along with general flow characteristics when designing platelet release bioreactors. In addition, we have demonstrated that our system can be scaled up to large volumes without loss of efficiency, a significant advantage for the industrialization of platelet culture. In conclusion, we have developed a platelet production device with a predictable and highly precise effect on each megakaryocyte. |
| format | Article |
| id | doaj-art-02a52fc153a748fc875bd7ef451b2328 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-02a52fc153a748fc875bd7ef451b23282025-01-19T12:17:49ZengNature PortfolioScientific Reports2045-23222025-01-0115111110.1038/s41598-024-79949-0Uniform impact on individual megakaryocytes is essential for efficient in vitro platelet productionAndrei K. Garzon Dasgupta0Anaïs Pongérard1Léa Mallo2Anita Eckly3François Lanza4Olivier Boiron5Yannick Knapp6Catherine Strassel7Université de Strasbourg, INSERM, EFS Grand-Est, BPPS UMR-S1255, FMTSUniversité de Strasbourg, INSERM, EFS Grand-Est, BPPS UMR-S1255, FMTSUniversité de Strasbourg, INSERM, EFS Grand-Est, BPPS UMR-S1255, FMTSUniversité de Strasbourg, INSERM, EFS Grand-Est, BPPS UMR-S1255, FMTSUniversité de Strasbourg, INSERM, EFS Grand-Est, BPPS UMR-S1255, FMTSCNRS, Université Aix-Marseille, Ecole Centrale Marseille, IRPHE UMR7342Université Avignon, LAPEC EA4278Université de Strasbourg, INSERM, EFS Grand-Est, BPPS UMR-S1255, FMTSAbstract Different approaches are being developed to efficiently produce in vitro platelets from cultured megakaryocytes to meet the constant demand of platelet transfusion and serve for research purposes. Recent works have shown that turbulence and periodic stress can significantly enhance platelet yield. Here we have developed and characterized a platelet production device that takes in account these properties. This device is based on the Taylor-Couette reactor in which a suspension is confined and sheared between two concentric cylinders. We have demonstrated that such a system allows obtaining high number of in vitro platelets per megakaryocyte with native-like morphology and functional properties. Using the combination of in silico and in vitro techniques, we claimed that overall turbulent conditions are not sufficient for efficient platelet release, and highlighted the importance of the uniform impact of flow on each megakaryocyte, a property that must be taken into account along with general flow characteristics when designing platelet release bioreactors. In addition, we have demonstrated that our system can be scaled up to large volumes without loss of efficiency, a significant advantage for the industrialization of platelet culture. In conclusion, we have developed a platelet production device with a predictable and highly precise effect on each megakaryocyte.https://doi.org/10.1038/s41598-024-79949-0Cultured plateletsCFD |
| spellingShingle | Andrei K. Garzon Dasgupta Anaïs Pongérard Léa Mallo Anita Eckly François Lanza Olivier Boiron Yannick Knapp Catherine Strassel Uniform impact on individual megakaryocytes is essential for efficient in vitro platelet production Scientific Reports Cultured platelets CFD |
| title | Uniform impact on individual megakaryocytes is essential for efficient in vitro platelet production |
| title_full | Uniform impact on individual megakaryocytes is essential for efficient in vitro platelet production |
| title_fullStr | Uniform impact on individual megakaryocytes is essential for efficient in vitro platelet production |
| title_full_unstemmed | Uniform impact on individual megakaryocytes is essential for efficient in vitro platelet production |
| title_short | Uniform impact on individual megakaryocytes is essential for efficient in vitro platelet production |
| title_sort | uniform impact on individual megakaryocytes is essential for efficient in vitro platelet production |
| topic | Cultured platelets CFD |
| url | https://doi.org/10.1038/s41598-024-79949-0 |
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