Protective effects of naringin against oxidative stress, inflammation, apoptosis, and DNA damage in rats with doxorubicin-induced hepatotoxicity
Objective: To investigate the protective effects of naringin on doxorubicin (DOX)-induced liver injury. Methods: A total of 50 male rats were allocated into five groups: the control group, the DOX group, the DOX groups treated with 50 mg/kg and 100 mg/kg of naringin by gastric lavage for 10 days, as...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-07-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/apjtb.apjtb_139_25 |
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| author | Pelin Durukan Azman Serkan Yildirim Emin Sengul Mohamad Warda Samet Tekin Furkan Aykurt Ali Cinar |
| author_facet | Pelin Durukan Azman Serkan Yildirim Emin Sengul Mohamad Warda Samet Tekin Furkan Aykurt Ali Cinar |
| author_sort | Pelin Durukan Azman |
| collection | DOAJ |
| description | Objective:
To investigate the protective effects of naringin on doxorubicin (DOX)-induced liver injury.
Methods:
A total of 50 male rats were allocated into five groups: the control group, the DOX group, the DOX groups treated with 50 mg/kg and 100 mg/kg of naringin by gastric lavage for 10 days, as well as the group treated with 100 mg/kg of naringin alone. Liver and serum samples were collected for biochemical, histopathological, and molecular analyses, including liver enzyme activity, oxidative stress markers, inflammation, apoptosis-related proteins, and DNA damage indicators.
Results:
Naringin attenuated DOX-induced elevation in liver enzyme activity and inflammation markers while enhancing antioxidant activities. Naringin also activated the Nrf2-HO-1 signaling pathway, with the most pronounced effect in the high-dose naringin group. In addition, naringin modulated apoptotic signaling by downregulating the expression of PI3K-AKT and BAX, and upregulating Bcl-2, as well as reduced the level of 8-OHdG. Histopathological evaluation showed that DOX-induced structural liver alterations, such as cellular degeneration and necrosis, were notably attenuated by naringin treatment.
Conclusions:
Naringin treatment exerts protective effects against DOX-induced liver injury through its antioxidative, anti-inflammatory, and anti-apoptotic effects. |
| format | Article |
| id | doaj-art-02911e1e6fcd42fdbf112fe40ca70dd4 |
| institution | Kabale University |
| issn | 2221-1691 2588-9222 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj-art-02911e1e6fcd42fdbf112fe40ca70dd42025-08-20T03:39:57ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222025-07-0115728529510.4103/apjtb.apjtb_139_25Protective effects of naringin against oxidative stress, inflammation, apoptosis, and DNA damage in rats with doxorubicin-induced hepatotoxicityPelin Durukan AzmanSerkan YildirimEmin SengulMohamad WardaSamet TekinFurkan AykurtAli CinarObjective: To investigate the protective effects of naringin on doxorubicin (DOX)-induced liver injury. Methods: A total of 50 male rats were allocated into five groups: the control group, the DOX group, the DOX groups treated with 50 mg/kg and 100 mg/kg of naringin by gastric lavage for 10 days, as well as the group treated with 100 mg/kg of naringin alone. Liver and serum samples were collected for biochemical, histopathological, and molecular analyses, including liver enzyme activity, oxidative stress markers, inflammation, apoptosis-related proteins, and DNA damage indicators. Results: Naringin attenuated DOX-induced elevation in liver enzyme activity and inflammation markers while enhancing antioxidant activities. Naringin also activated the Nrf2-HO-1 signaling pathway, with the most pronounced effect in the high-dose naringin group. In addition, naringin modulated apoptotic signaling by downregulating the expression of PI3K-AKT and BAX, and upregulating Bcl-2, as well as reduced the level of 8-OHdG. Histopathological evaluation showed that DOX-induced structural liver alterations, such as cellular degeneration and necrosis, were notably attenuated by naringin treatment. Conclusions: Naringin treatment exerts protective effects against DOX-induced liver injury through its antioxidative, anti-inflammatory, and anti-apoptotic effects.https://journals.lww.com/10.4103/apjtb.apjtb_139_25doxorubicinhepatotoxicityinflammationnaringinoxidative stress |
| spellingShingle | Pelin Durukan Azman Serkan Yildirim Emin Sengul Mohamad Warda Samet Tekin Furkan Aykurt Ali Cinar Protective effects of naringin against oxidative stress, inflammation, apoptosis, and DNA damage in rats with doxorubicin-induced hepatotoxicity Asian Pacific Journal of Tropical Biomedicine doxorubicin hepatotoxicity inflammation naringin oxidative stress |
| title | Protective effects of naringin against oxidative stress, inflammation, apoptosis, and DNA damage in rats with doxorubicin-induced hepatotoxicity |
| title_full | Protective effects of naringin against oxidative stress, inflammation, apoptosis, and DNA damage in rats with doxorubicin-induced hepatotoxicity |
| title_fullStr | Protective effects of naringin against oxidative stress, inflammation, apoptosis, and DNA damage in rats with doxorubicin-induced hepatotoxicity |
| title_full_unstemmed | Protective effects of naringin against oxidative stress, inflammation, apoptosis, and DNA damage in rats with doxorubicin-induced hepatotoxicity |
| title_short | Protective effects of naringin against oxidative stress, inflammation, apoptosis, and DNA damage in rats with doxorubicin-induced hepatotoxicity |
| title_sort | protective effects of naringin against oxidative stress inflammation apoptosis and dna damage in rats with doxorubicin induced hepatotoxicity |
| topic | doxorubicin hepatotoxicity inflammation naringin oxidative stress |
| url | https://journals.lww.com/10.4103/apjtb.apjtb_139_25 |
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