Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin: NMR, Fluorescence, and FTIR Approaches
Nicotine, the important component of cigarette products, may have an impact on the oral environment after inhalation. The research of interaction between nicotine and bovine submaxillary mucin (BSM) contributes to understand the binding mechanism of nicotine and BSM, and the effects of nicotine on t...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2016/9580136 |
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| author | Xiaoxiang Liao Dalin Yuan Jianguo Tang Hongqin Yang Bing Liang Qiang Cheng Hui Li |
| author_facet | Xiaoxiang Liao Dalin Yuan Jianguo Tang Hongqin Yang Bing Liang Qiang Cheng Hui Li |
| author_sort | Xiaoxiang Liao |
| collection | DOAJ |
| description | Nicotine, the important component of cigarette products, may have an impact on the oral environment after inhalation. The research of interaction between nicotine and bovine submaxillary mucin (BSM) contributes to understand the binding mechanism of nicotine and BSM, and the effects of nicotine on the structure and function of the mucin. NMR data demonstrated that the interaction between nicotine and BSM did exist, and it was pyrrolidyl ring of nicotine playing the major role in the binding. The quenching mechanisms of nicotine and BSM in different pH were different: for acidic environment, the quenching was dynamic; while it became static in the alkaline circumstance. Synchronous fluorescence spectra indicated that nicotine had effect on the microenvironment of the Trp rather than Tyr residue. Meanwhile, the impact of nicotine on the conformation of BSM was also confirmed by 3D fluorescence and FTIR spectra. |
| format | Article |
| id | doaj-art-02900f1bf2ca42a688fddd7bff3fa63d |
| institution | OA Journals |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-02900f1bf2ca42a688fddd7bff3fa63d2025-08-20T02:03:27ZengWileyJournal of Spectroscopy2314-49202314-49392016-01-01201610.1155/2016/95801369580136Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin: NMR, Fluorescence, and FTIR ApproachesXiaoxiang Liao0Dalin Yuan1Jianguo Tang2Hongqin Yang3Bing Liang4Qiang Cheng5Hui Li6College of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, ChinaTechnology Center, China Tobacco Yunnan Industrial Co., Ltd., Kunming, Yunnan 650204, ChinaTechnology Center, China Tobacco Yunnan Industrial Co., Ltd., Kunming, Yunnan 650204, ChinaCollege of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, ChinaCollege of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, ChinaCollege of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, ChinaCollege of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, ChinaNicotine, the important component of cigarette products, may have an impact on the oral environment after inhalation. The research of interaction between nicotine and bovine submaxillary mucin (BSM) contributes to understand the binding mechanism of nicotine and BSM, and the effects of nicotine on the structure and function of the mucin. NMR data demonstrated that the interaction between nicotine and BSM did exist, and it was pyrrolidyl ring of nicotine playing the major role in the binding. The quenching mechanisms of nicotine and BSM in different pH were different: for acidic environment, the quenching was dynamic; while it became static in the alkaline circumstance. Synchronous fluorescence spectra indicated that nicotine had effect on the microenvironment of the Trp rather than Tyr residue. Meanwhile, the impact of nicotine on the conformation of BSM was also confirmed by 3D fluorescence and FTIR spectra.http://dx.doi.org/10.1155/2016/9580136 |
| spellingShingle | Xiaoxiang Liao Dalin Yuan Jianguo Tang Hongqin Yang Bing Liang Qiang Cheng Hui Li Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin: NMR, Fluorescence, and FTIR Approaches Journal of Spectroscopy |
| title | Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin: NMR, Fluorescence, and FTIR Approaches |
| title_full | Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin: NMR, Fluorescence, and FTIR Approaches |
| title_fullStr | Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin: NMR, Fluorescence, and FTIR Approaches |
| title_full_unstemmed | Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin: NMR, Fluorescence, and FTIR Approaches |
| title_short | Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin: NMR, Fluorescence, and FTIR Approaches |
| title_sort | probing into the interaction of nicotine and bovine submaxillary mucin nmr fluorescence and ftir approaches |
| url | http://dx.doi.org/10.1155/2016/9580136 |
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