The potential of strigolactones to shift competitive dynamics among two Rhizophagus irregularis strains
Strigolactones are phytohormones that influence arbuscular mycorrhizal fungal (AMF) spore germination, pre-symbiotic hyphal branching, and metabolic rates. Historically, strigolactone effects have been tested on single AMF strains. An open question is whether intraspecific variation in strigolactone...
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Frontiers Media S.A.
2024-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1470469/full |
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| author | Malin Klein Malin Klein Corentin Bisot Loreto Oyarte Gálvez Loreto Oyarte Gálvez Vasilis Kokkoris Thomas S. Shimizu Lemeng Dong James T. Weedon Harro Bouwmeester E. Toby Kiers |
| author_facet | Malin Klein Malin Klein Corentin Bisot Loreto Oyarte Gálvez Loreto Oyarte Gálvez Vasilis Kokkoris Thomas S. Shimizu Lemeng Dong James T. Weedon Harro Bouwmeester E. Toby Kiers |
| author_sort | Malin Klein |
| collection | DOAJ |
| description | Strigolactones are phytohormones that influence arbuscular mycorrhizal fungal (AMF) spore germination, pre-symbiotic hyphal branching, and metabolic rates. Historically, strigolactone effects have been tested on single AMF strains. An open question is whether intraspecific variation in strigolactone effects and intraspecific interactions can influence AMF competition. Using the Rhizophagus irregularis strains A5 and C2, we tested for intraspecific variation in the response of germination and pre-symbiotic growth (i.e., hyphal length and branching) to the strigolactones GR24 and 5-deoxystrigol. We also tested if interactions between these strains modified their germination rates and pre-symbiotic growth. Spore germination rates were consistently high (> 90%) for C2 spores, regardless of treatment and the presence of the other strain. For A5 spores, germination was increased by strigolactone presence from approximately 30 to 70% but reduced when grown in mixed culture. When growing together, branching increased for both strains compared to monocultures. In mixed cultures, strigolactones increased the branching for both strains but led to an increase in hyphal length only for the strain A5. These strain-specific responses suggest that strigolactones may have the potential to shift competitive dynamics among AMF species with direct implications for the establishment of the AMF community. |
| format | Article |
| id | doaj-art-0287fee9b7bd460aabeed8d9aefe6d48 |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-0287fee9b7bd460aabeed8d9aefe6d482025-08-20T02:15:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-10-011510.3389/fmicb.2024.14704691470469The potential of strigolactones to shift competitive dynamics among two Rhizophagus irregularis strainsMalin Klein0Malin Klein1Corentin Bisot2Loreto Oyarte Gálvez3Loreto Oyarte Gálvez4Vasilis Kokkoris5Thomas S. Shimizu6Lemeng Dong7James T. Weedon8Harro Bouwmeester9E. Toby Kiers10Section of Ecology and Evolution, Amsterdam Institute for Life and Environment, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsPlant Hormone Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, NetherlandsPhysics of Behavior, AMOLF Institute, Amsterdam, NetherlandsSection of Ecology and Evolution, Amsterdam Institute for Life and Environment, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsPhysics of Behavior, AMOLF Institute, Amsterdam, NetherlandsSection of Systems Ecology, Amsterdam Institute for Life and Environment, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsPhysics of Behavior, AMOLF Institute, Amsterdam, NetherlandsPlant Hormone Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, NetherlandsSection of Systems Ecology, Amsterdam Institute for Life and Environment, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsPlant Hormone Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, NetherlandsSection of Ecology and Evolution, Amsterdam Institute for Life and Environment, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsStrigolactones are phytohormones that influence arbuscular mycorrhizal fungal (AMF) spore germination, pre-symbiotic hyphal branching, and metabolic rates. Historically, strigolactone effects have been tested on single AMF strains. An open question is whether intraspecific variation in strigolactone effects and intraspecific interactions can influence AMF competition. Using the Rhizophagus irregularis strains A5 and C2, we tested for intraspecific variation in the response of germination and pre-symbiotic growth (i.e., hyphal length and branching) to the strigolactones GR24 and 5-deoxystrigol. We also tested if interactions between these strains modified their germination rates and pre-symbiotic growth. Spore germination rates were consistently high (> 90%) for C2 spores, regardless of treatment and the presence of the other strain. For A5 spores, germination was increased by strigolactone presence from approximately 30 to 70% but reduced when grown in mixed culture. When growing together, branching increased for both strains compared to monocultures. In mixed cultures, strigolactones increased the branching for both strains but led to an increase in hyphal length only for the strain A5. These strain-specific responses suggest that strigolactones may have the potential to shift competitive dynamics among AMF species with direct implications for the establishment of the AMF community.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1470469/fullstrigolactonesarbuscular mycorrhizal fungirhizosphereintraspecific variationintraspecific interactiongermination |
| spellingShingle | Malin Klein Malin Klein Corentin Bisot Loreto Oyarte Gálvez Loreto Oyarte Gálvez Vasilis Kokkoris Thomas S. Shimizu Lemeng Dong James T. Weedon Harro Bouwmeester E. Toby Kiers The potential of strigolactones to shift competitive dynamics among two Rhizophagus irregularis strains Frontiers in Microbiology strigolactones arbuscular mycorrhizal fungi rhizosphere intraspecific variation intraspecific interaction germination |
| title | The potential of strigolactones to shift competitive dynamics among two Rhizophagus irregularis strains |
| title_full | The potential of strigolactones to shift competitive dynamics among two Rhizophagus irregularis strains |
| title_fullStr | The potential of strigolactones to shift competitive dynamics among two Rhizophagus irregularis strains |
| title_full_unstemmed | The potential of strigolactones to shift competitive dynamics among two Rhizophagus irregularis strains |
| title_short | The potential of strigolactones to shift competitive dynamics among two Rhizophagus irregularis strains |
| title_sort | potential of strigolactones to shift competitive dynamics among two rhizophagus irregularis strains |
| topic | strigolactones arbuscular mycorrhizal fungi rhizosphere intraspecific variation intraspecific interaction germination |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1470469/full |
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