State of the Art in the Standardization of Stromal Vascular Fraction Processing
Stromal Vascular Fraction (SVF) has gained significant attention in clinical applications due to its regenerative and anti-inflammatory properties. Initially identified decades ago, SVF is derived from adipose tissue and has been increasingly utilized in a variety of therapeutic settings. The isolat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/15/2/199 |
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| author | Martina Cremona Matteo Gallazzi Giulio Rusconi Luca Mariotta Mauro Gola Gianni Soldati |
| author_facet | Martina Cremona Matteo Gallazzi Giulio Rusconi Luca Mariotta Mauro Gola Gianni Soldati |
| author_sort | Martina Cremona |
| collection | DOAJ |
| description | Stromal Vascular Fraction (SVF) has gained significant attention in clinical applications due to its regenerative and anti-inflammatory properties. Initially identified decades ago, SVF is derived from adipose tissue and has been increasingly utilized in a variety of therapeutic settings. The isolation and processing protocols for SVF have evolved substantially, particularly after its classification as an Advanced Therapy Medicinal Product (ATMP), which mandates adherence to Good Manufacturing Practices to ensure sterility and product quality. Despite the progress, few studies over the last decade have focused on the standardization of SVF processing. Recent advances, driven by the potential of SVF and its derived products such as Adipose-derived Stem Cells, have prompted the development of improved isolation strategies aimed at enhancing their therapeutic and regenerative efficacy. Notable progress includes the advent of automated processing systems, which reduce technical errors, minimize variability, and improve reproducibility across laboratories. These developments, along with the establishment of more precise protocols and guidelines, have enhanced the consistency and clinical applicability of SVF-based therapies. This review discusses the key aspects of SVF isolation and processing, highlighting the efforts to standardize the procedure and ensure the reliability of SVF products for clinical use. |
| format | Article |
| id | doaj-art-02842a1fcff4487c801e77b7c42cc024 |
| institution | DOAJ |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-02842a1fcff4487c801e77b7c42cc0242025-08-20T02:44:59ZengMDPI AGBiomolecules2218-273X2025-01-0115219910.3390/biom15020199State of the Art in the Standardization of Stromal Vascular Fraction ProcessingMartina Cremona0Matteo Gallazzi1Giulio Rusconi2Luca Mariotta3Mauro Gola4Gianni Soldati5Swiss Stem Cell Foundation, 6900 Lugano, SwitzerlandSwiss Stem Cell Foundation, 6900 Lugano, SwitzerlandSwiss Stem Cell Foundation, 6900 Lugano, SwitzerlandSwiss Stem Cell Foundation, 6900 Lugano, SwitzerlandSwiss Stem Cell Foundation, 6900 Lugano, SwitzerlandSwiss Stem Cell Foundation, 6900 Lugano, SwitzerlandStromal Vascular Fraction (SVF) has gained significant attention in clinical applications due to its regenerative and anti-inflammatory properties. Initially identified decades ago, SVF is derived from adipose tissue and has been increasingly utilized in a variety of therapeutic settings. The isolation and processing protocols for SVF have evolved substantially, particularly after its classification as an Advanced Therapy Medicinal Product (ATMP), which mandates adherence to Good Manufacturing Practices to ensure sterility and product quality. Despite the progress, few studies over the last decade have focused on the standardization of SVF processing. Recent advances, driven by the potential of SVF and its derived products such as Adipose-derived Stem Cells, have prompted the development of improved isolation strategies aimed at enhancing their therapeutic and regenerative efficacy. Notable progress includes the advent of automated processing systems, which reduce technical errors, minimize variability, and improve reproducibility across laboratories. These developments, along with the establishment of more precise protocols and guidelines, have enhanced the consistency and clinical applicability of SVF-based therapies. This review discusses the key aspects of SVF isolation and processing, highlighting the efforts to standardize the procedure and ensure the reliability of SVF products for clinical use.https://www.mdpi.com/2218-273X/15/2/199adipose tissueStromal Vascular FractionGMPadipose tissue processingcryopreservationquality controls |
| spellingShingle | Martina Cremona Matteo Gallazzi Giulio Rusconi Luca Mariotta Mauro Gola Gianni Soldati State of the Art in the Standardization of Stromal Vascular Fraction Processing Biomolecules adipose tissue Stromal Vascular Fraction GMP adipose tissue processing cryopreservation quality controls |
| title | State of the Art in the Standardization of Stromal Vascular Fraction Processing |
| title_full | State of the Art in the Standardization of Stromal Vascular Fraction Processing |
| title_fullStr | State of the Art in the Standardization of Stromal Vascular Fraction Processing |
| title_full_unstemmed | State of the Art in the Standardization of Stromal Vascular Fraction Processing |
| title_short | State of the Art in the Standardization of Stromal Vascular Fraction Processing |
| title_sort | state of the art in the standardization of stromal vascular fraction processing |
| topic | adipose tissue Stromal Vascular Fraction GMP adipose tissue processing cryopreservation quality controls |
| url | https://www.mdpi.com/2218-273X/15/2/199 |
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