Environmental drivers of fungal community structure: a comparative high-throughput sequencing study of two geothermally distinct hot springs in Hefei, China

IntroductionThere are abundant hot spring resources in Hefei. This is the first study to analyze and compare the fungal diversity of Tangchi and Bantang hot springs in Hefei.MethodsThis study represents the comprehensive analysis of fungal diversity in two geothermally distinct hot springs (Tangchi...

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Main Author: Feng-Qin Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1477445/full
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author Feng-Qin Zhang
author_facet Feng-Qin Zhang
author_sort Feng-Qin Zhang
collection DOAJ
description IntroductionThere are abundant hot spring resources in Hefei. This is the first study to analyze and compare the fungal diversity of Tangchi and Bantang hot springs in Hefei.MethodsThis study represents the comprehensive analysis of fungal diversity in two geothermally distinct hot springs (Tangchi and Bantang) in Hefei, China, using Illumina MiSeq PE300 sequencing of the ITS region.ResultsDespite their spatial proximity, Tangchi (63.9°C, pH 8.2) and Bantang (45.8°C, pH 6.5) showed stark contrasts in fungal community composition. A total of 934 OTUs were identified, with Tangchi exhibiting greater α-diversity and only 18.6% OTU overlap. Both systems were dominated by Ascomycota and Basidiomycota, yet genus-level divergences were substantial: Tangchi favored Rhizophydium, Aureobasidium, and Rhodotorula, while Bantang harbored Coniosporium, Trichomerium, and Aureobasidium.DiscussionComparative analysis with freshwater ecosystems revealed unique thermoadaptive strategies and reduced overlap with non-thermal fungal assemblages. These findings emphasize temperature and pH as critical drivers of fungal niche partitioning in geothermal systems and underscore the untapped biotechnological potential of thermophilic fungi.
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spelling doaj-art-02684fad025d497a8d135dc8de46b9502025-08-20T03:30:41ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.14774451477445Environmental drivers of fungal community structure: a comparative high-throughput sequencing study of two geothermally distinct hot springs in Hefei, ChinaFeng-Qin ZhangIntroductionThere are abundant hot spring resources in Hefei. This is the first study to analyze and compare the fungal diversity of Tangchi and Bantang hot springs in Hefei.MethodsThis study represents the comprehensive analysis of fungal diversity in two geothermally distinct hot springs (Tangchi and Bantang) in Hefei, China, using Illumina MiSeq PE300 sequencing of the ITS region.ResultsDespite their spatial proximity, Tangchi (63.9°C, pH 8.2) and Bantang (45.8°C, pH 6.5) showed stark contrasts in fungal community composition. A total of 934 OTUs were identified, with Tangchi exhibiting greater α-diversity and only 18.6% OTU overlap. Both systems were dominated by Ascomycota and Basidiomycota, yet genus-level divergences were substantial: Tangchi favored Rhizophydium, Aureobasidium, and Rhodotorula, while Bantang harbored Coniosporium, Trichomerium, and Aureobasidium.DiscussionComparative analysis with freshwater ecosystems revealed unique thermoadaptive strategies and reduced overlap with non-thermal fungal assemblages. These findings emphasize temperature and pH as critical drivers of fungal niche partitioning in geothermal systems and underscore the untapped biotechnological potential of thermophilic fungi.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1477445/fullfungal community structureenvironmental drivershigh-throughput sequencingBantang hot springTangchi hot spring
spellingShingle Feng-Qin Zhang
Environmental drivers of fungal community structure: a comparative high-throughput sequencing study of two geothermally distinct hot springs in Hefei, China
Frontiers in Microbiology
fungal community structure
environmental drivers
high-throughput sequencing
Bantang hot spring
Tangchi hot spring
title Environmental drivers of fungal community structure: a comparative high-throughput sequencing study of two geothermally distinct hot springs in Hefei, China
title_full Environmental drivers of fungal community structure: a comparative high-throughput sequencing study of two geothermally distinct hot springs in Hefei, China
title_fullStr Environmental drivers of fungal community structure: a comparative high-throughput sequencing study of two geothermally distinct hot springs in Hefei, China
title_full_unstemmed Environmental drivers of fungal community structure: a comparative high-throughput sequencing study of two geothermally distinct hot springs in Hefei, China
title_short Environmental drivers of fungal community structure: a comparative high-throughput sequencing study of two geothermally distinct hot springs in Hefei, China
title_sort environmental drivers of fungal community structure a comparative high throughput sequencing study of two geothermally distinct hot springs in hefei china
topic fungal community structure
environmental drivers
high-throughput sequencing
Bantang hot spring
Tangchi hot spring
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1477445/full
work_keys_str_mv AT fengqinzhang environmentaldriversoffungalcommunitystructureacomparativehighthroughputsequencingstudyoftwogeothermallydistincthotspringsinhefeichina