Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors
High-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, and environmental monitoring. CRISPR/Cas systems have emerged as transformative tools in biosensing due to their unparalleled specificity, programmability, and unique e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-03-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/3/155 |
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| author | Xianglin Xin Jing Su Haoran Cui Lihua Wang Shiping Song |
| author_facet | Xianglin Xin Jing Su Haoran Cui Lihua Wang Shiping Song |
| author_sort | Xianglin Xin |
| collection | DOAJ |
| description | High-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, and environmental monitoring. CRISPR/Cas systems have emerged as transformative tools in biosensing due to their unparalleled specificity, programmability, and unique enzymatic activities. They exhibit two key cleavage behaviors: precise ON-target cleavage guided by specific protospacers, which ensures accurate target recognition, and bystander cleavage activity triggered upon target binding, which enables robust signal amplification. These properties make CRISPR/Cas systems highly versatile for designing biosensors for ultra-sensitive detection. This review comprehensively explores recent advancements in CRISPR/Cas system-based biosensors, highlighting their impact on improving biosensing performance. We discuss the integration of CRISPR/Cas systems with diverse signal readout mechanisms, including electrochemical, fluorescent, colorimetric, surface-enhanced Raman scattering (SERS), and so on. Additionally, we examine the development of integrated biosensing systems, such as microfluidic devices and portable biosensors, which leverage CRISPR/Cas technology for point-of-care testing (POCT) and high-throughput analysis. Furthermore, we identify unresolved challenges, aiming to inspire innovative solutions and accelerate the translation of these technologies into practical applications for diagnostics, food, and environment safety. |
| format | Article |
| id | doaj-art-0266dc6077bb46d1a0fd042d4cef16ce |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-0266dc6077bb46d1a0fd042d4cef16ce2025-08-20T03:43:37ZengMDPI AGBiosensors2079-63742025-03-0115315510.3390/bios15030155Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based BiosensorsXianglin Xin0Jing Su1Haoran Cui2Lihua Wang3Shiping Song4Institute of Materiobiology, College of Sciences, Shanghai University, Shanghai 200444, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, No. 100 Haiquan Road, Shanghai 201418, ChinaInstitute of Materiobiology, College of Sciences, Shanghai University, Shanghai 200444, ChinaInstitute of Materiobiology, College of Sciences, Shanghai University, Shanghai 200444, ChinaInstitute of Materiobiology, College of Sciences, Shanghai University, Shanghai 200444, ChinaHigh-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, and environmental monitoring. CRISPR/Cas systems have emerged as transformative tools in biosensing due to their unparalleled specificity, programmability, and unique enzymatic activities. They exhibit two key cleavage behaviors: precise ON-target cleavage guided by specific protospacers, which ensures accurate target recognition, and bystander cleavage activity triggered upon target binding, which enables robust signal amplification. These properties make CRISPR/Cas systems highly versatile for designing biosensors for ultra-sensitive detection. This review comprehensively explores recent advancements in CRISPR/Cas system-based biosensors, highlighting their impact on improving biosensing performance. We discuss the integration of CRISPR/Cas systems with diverse signal readout mechanisms, including electrochemical, fluorescent, colorimetric, surface-enhanced Raman scattering (SERS), and so on. Additionally, we examine the development of integrated biosensing systems, such as microfluidic devices and portable biosensors, which leverage CRISPR/Cas technology for point-of-care testing (POCT) and high-throughput analysis. Furthermore, we identify unresolved challenges, aiming to inspire innovative solutions and accelerate the translation of these technologies into practical applications for diagnostics, food, and environment safety.https://www.mdpi.com/2079-6374/15/3/155CRISPR/Cas systembiosensorsbiosensing systemsdiagnostics |
| spellingShingle | Xianglin Xin Jing Su Haoran Cui Lihua Wang Shiping Song Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors Biosensors CRISPR/Cas system biosensors biosensing systems diagnostics |
| title | Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors |
| title_full | Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors |
| title_fullStr | Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors |
| title_full_unstemmed | Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors |
| title_short | Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors |
| title_sort | recent advances in clustered regularly interspaced short palindromic repeats crispr associated proteins system based biosensors |
| topic | CRISPR/Cas system biosensors biosensing systems diagnostics |
| url | https://www.mdpi.com/2079-6374/15/3/155 |
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