Structural Features and Transcriptional Activity of Chicken PPARs (α, β, and γ)
While an understanding of lipid metabolism in chickens is critical for a further improvement of food production, there are few studies concerning differences in lipid metabolism mechanisms between chickens and other species at a molecular level. Chickens have three PPAR gene subtypes (α, β, and γ) t...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2013/186312 |
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| author | Ichiro Takada Mime Kobayashi |
| author_facet | Ichiro Takada Mime Kobayashi |
| author_sort | Ichiro Takada |
| collection | DOAJ |
| description | While an understanding of lipid metabolism in chickens is critical for a further improvement of food production, there are few studies concerning differences in lipid metabolism mechanisms between chickens and other species at a molecular level. Chickens have three PPAR gene subtypes (α, β, and γ) that function differently from those present in humans and mice. The chicken PPAR-gamma (cPPARγ) gene is shorter than that in humans and lacks a γ2 isoform. Moreover, in serum-free media, cPPARγ shows high transcriptional activity without exogenous ligands. Luciferase reporter assays were used to examine the effect of sera on cPPAR transcriptional activities and showed that adult bovine serum and chicken serum highly activate cPPARα and β functions. Moreover, we found that bezafibrate induces the transactivation function of cPPARβ, but not human PPARδ (human PPARβ ortholog). This ligand selectivity relies on one amino acid residue (chicken: Val419, human: Met444). These results show the possibilities for unique functions of cPPARs on chicken-specific lipid glucose metabolism. As such, a better understanding of the molecular mechanisms of lipid metabolism in chickens could result in higher productivity for the poultry industry. |
| format | Article |
| id | doaj-art-026463712065425ea8ae62013401f556 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-026463712065425ea8ae62013401f5562025-02-03T01:27:49ZengWileyPPAR Research1687-47571687-47652013-01-01201310.1155/2013/186312186312Structural Features and Transcriptional Activity of Chicken PPARs (α, β, and γ)Ichiro Takada0Mime Kobayashi1Department of Cell and Tissue Biology, School of Medicine, Keio University, Tokyo 160-8582, JapanGraduate School of Biological Sciences, Nara Institute of Science and Technology, Nara 630-0192, JapanWhile an understanding of lipid metabolism in chickens is critical for a further improvement of food production, there are few studies concerning differences in lipid metabolism mechanisms between chickens and other species at a molecular level. Chickens have three PPAR gene subtypes (α, β, and γ) that function differently from those present in humans and mice. The chicken PPAR-gamma (cPPARγ) gene is shorter than that in humans and lacks a γ2 isoform. Moreover, in serum-free media, cPPARγ shows high transcriptional activity without exogenous ligands. Luciferase reporter assays were used to examine the effect of sera on cPPAR transcriptional activities and showed that adult bovine serum and chicken serum highly activate cPPARα and β functions. Moreover, we found that bezafibrate induces the transactivation function of cPPARβ, but not human PPARδ (human PPARβ ortholog). This ligand selectivity relies on one amino acid residue (chicken: Val419, human: Met444). These results show the possibilities for unique functions of cPPARs on chicken-specific lipid glucose metabolism. As such, a better understanding of the molecular mechanisms of lipid metabolism in chickens could result in higher productivity for the poultry industry.http://dx.doi.org/10.1155/2013/186312 |
| spellingShingle | Ichiro Takada Mime Kobayashi Structural Features and Transcriptional Activity of Chicken PPARs (α, β, and γ) PPAR Research |
| title | Structural Features and Transcriptional Activity of Chicken PPARs (α, β, and γ) |
| title_full | Structural Features and Transcriptional Activity of Chicken PPARs (α, β, and γ) |
| title_fullStr | Structural Features and Transcriptional Activity of Chicken PPARs (α, β, and γ) |
| title_full_unstemmed | Structural Features and Transcriptional Activity of Chicken PPARs (α, β, and γ) |
| title_short | Structural Features and Transcriptional Activity of Chicken PPARs (α, β, and γ) |
| title_sort | structural features and transcriptional activity of chicken ppars α β and γ |
| url | http://dx.doi.org/10.1155/2013/186312 |
| work_keys_str_mv | AT ichirotakada structuralfeaturesandtranscriptionalactivityofchickenpparsabandg AT mimekobayashi structuralfeaturesandtranscriptionalactivityofchickenpparsabandg |