5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils
The roles of eosinophils in antimicrobial defense remain incompletely understood. In ovalbumin-sensitized mice, eosinophils are selectively recruited to the peritoneal cavity by antigen, eotaxin, or leukotriene(LT)B4, a 5-lipoxygenase (5-LO) metabolite. 5-LO blockade prevents responses to both antig...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/102160 |
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| author | Ricardo Alves Luz Pedro Xavier-Elsas Bianca de Luca Daniela Masid-de-Brito Priscila Soares Cauduro Luiz Carlos Gondar Arcanjo Ana Carolina Cordeiro Faria dos Santos Ivi Cristina Maria de Oliveira Maria Ignez Capella Gaspar-Elsas |
| author_facet | Ricardo Alves Luz Pedro Xavier-Elsas Bianca de Luca Daniela Masid-de-Brito Priscila Soares Cauduro Luiz Carlos Gondar Arcanjo Ana Carolina Cordeiro Faria dos Santos Ivi Cristina Maria de Oliveira Maria Ignez Capella Gaspar-Elsas |
| author_sort | Ricardo Alves Luz |
| collection | DOAJ |
| description | The roles of eosinophils in antimicrobial defense remain incompletely understood. In ovalbumin-sensitized mice, eosinophils are selectively recruited to the peritoneal cavity by antigen, eotaxin, or leukotriene(LT)B4, a 5-lipoxygenase (5-LO) metabolite. 5-LO blockade prevents responses to both antigen and eotaxin. We examined responses to eotaxin in the absence of sensitization and their dependence on 5-LO. BALB/c or PAS mice and their mutants (5-LO-deficient ALOX; eosinophil-deficient GATA-1) were injected i.p. with eotaxin, eosinophils, or both, and leukocyte accumulation was quantified up to 24 h. Significant recruitment of eosinophils by eotaxin in BALB/c, up to 24 h, was accompanied by much larger numbers of recruited neutrophils and monocytes/macrophages. These effects were abolished by eotaxin neutralization and 5-LO-activating protein inhibitor MK886. In ALOX (but not PAS) mice, eotaxin recruitment was abolished for eosinophils and halved for neutrophils. In GATA-1 mutants, eotaxin recruited neither neutrophils nor macrophages. Transfer of eosinophils cultured from bone-marrow of BALB/c donors, or from ALOX donors, into GATA-1 mutant recipients, i.p., restored eotaxin recruitment of neutrophils and showed that the critical step dependent on 5-LO is the initial recruitment of eosinophils by eotaxin, not the secondary neutrophil accumulation. Eosinophil-dependent recruitment of neutrophils in naive BALB/c mice was associated with increased binding of bacteria. |
| format | Article |
| id | doaj-art-023cece4796f45d2bd99670e4ba83cdc |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-023cece4796f45d2bd99670e4ba83cdc2025-02-03T01:12:26ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/1021601021605-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine EosinophilsRicardo Alves Luz0Pedro Xavier-Elsas1Bianca de Luca2Daniela Masid-de-Brito3Priscila Soares Cauduro4Luiz Carlos Gondar Arcanjo5Ana Carolina Cordeiro Faria dos Santos6Ivi Cristina Maria de Oliveira7Maria Ignez Capella Gaspar-Elsas8Department of Immunology, IMPG, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Room I-2-066, 21941-590 Rio de Janeiro, BrazilDepartment of Immunology, IMPG, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Room I-2-066, 21941-590 Rio de Janeiro, BrazilDepartment of Pediatrics, IFF, FIOCRUZ, 22250-020 Rio de Janeiro, BrazilDepartment of Immunology, IMPG, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Room I-2-066, 21941-590 Rio de Janeiro, BrazilDepartment of Immunology, IMPG, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Room I-2-066, 21941-590 Rio de Janeiro, BrazilDepartment of Immunology, IMPG, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Room I-2-066, 21941-590 Rio de Janeiro, BrazilDepartment of Immunology, IMPG, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Room I-2-066, 21941-590 Rio de Janeiro, BrazilDepartment of Medical Microbiology, IMPG, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, BrazilDepartment of Pediatrics, IFF, FIOCRUZ, 22250-020 Rio de Janeiro, BrazilThe roles of eosinophils in antimicrobial defense remain incompletely understood. In ovalbumin-sensitized mice, eosinophils are selectively recruited to the peritoneal cavity by antigen, eotaxin, or leukotriene(LT)B4, a 5-lipoxygenase (5-LO) metabolite. 5-LO blockade prevents responses to both antigen and eotaxin. We examined responses to eotaxin in the absence of sensitization and their dependence on 5-LO. BALB/c or PAS mice and their mutants (5-LO-deficient ALOX; eosinophil-deficient GATA-1) were injected i.p. with eotaxin, eosinophils, or both, and leukocyte accumulation was quantified up to 24 h. Significant recruitment of eosinophils by eotaxin in BALB/c, up to 24 h, was accompanied by much larger numbers of recruited neutrophils and monocytes/macrophages. These effects were abolished by eotaxin neutralization and 5-LO-activating protein inhibitor MK886. In ALOX (but not PAS) mice, eotaxin recruitment was abolished for eosinophils and halved for neutrophils. In GATA-1 mutants, eotaxin recruited neither neutrophils nor macrophages. Transfer of eosinophils cultured from bone-marrow of BALB/c donors, or from ALOX donors, into GATA-1 mutant recipients, i.p., restored eotaxin recruitment of neutrophils and showed that the critical step dependent on 5-LO is the initial recruitment of eosinophils by eotaxin, not the secondary neutrophil accumulation. Eosinophil-dependent recruitment of neutrophils in naive BALB/c mice was associated with increased binding of bacteria.http://dx.doi.org/10.1155/2014/102160 |
| spellingShingle | Ricardo Alves Luz Pedro Xavier-Elsas Bianca de Luca Daniela Masid-de-Brito Priscila Soares Cauduro Luiz Carlos Gondar Arcanjo Ana Carolina Cordeiro Faria dos Santos Ivi Cristina Maria de Oliveira Maria Ignez Capella Gaspar-Elsas 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils Mediators of Inflammation |
| title | 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
| title_full | 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
| title_fullStr | 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
| title_full_unstemmed | 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
| title_short | 5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils |
| title_sort | 5 lipoxygenase dependent recruitment of neutrophils and macrophages by eotaxin stimulated murine eosinophils |
| url | http://dx.doi.org/10.1155/2014/102160 |
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