Bifidobacterium longum JBLC-141 alleviates hypobaric hypoxia-induced intestinal barrier damage by attenuating inflammatory responses and oxidative stress
Hypobaric hypoxia exposure occurs at high altitudes, including plateaus, and affects normal intestinal function and microbiota composition. Exposure induces an intestinal inflammatory response and oxidative stress injury, ultimately disrupting intestinal homeostasis and causing barrier damage. Thus,...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1501999/full |
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| author | Xiang-Yang Li Xiang-Yang Li Xiang-Yang Li Jin Shang Jin Shang Jin Shang Xiao-Juan Wang Xiao-Juan Wang Xiao-Juan Wang Hui-Ping Ma Long-Fei Ren Long-Fei Ren Long-Fei Ren Long-Fei Ren Long-Fei Ren Lei Zhang Lei Zhang Lei Zhang Lei Zhang Lei Zhang |
| author_facet | Xiang-Yang Li Xiang-Yang Li Xiang-Yang Li Jin Shang Jin Shang Jin Shang Xiao-Juan Wang Xiao-Juan Wang Xiao-Juan Wang Hui-Ping Ma Long-Fei Ren Long-Fei Ren Long-Fei Ren Long-Fei Ren Long-Fei Ren Lei Zhang Lei Zhang Lei Zhang Lei Zhang Lei Zhang |
| author_sort | Xiang-Yang Li |
| collection | DOAJ |
| description | Hypobaric hypoxia exposure occurs at high altitudes, including plateaus, and affects normal intestinal function and microbiota composition. Exposure induces an intestinal inflammatory response and oxidative stress injury, ultimately disrupting intestinal homeostasis and causing barrier damage. Thus, due to its anti-inflammatory, antioxidative, and intestinal microbiota-regulating properties, Bifidobacterium longum is a potentially effective probiotic intervention to protect the intestinal barrier during low-pressure hypoxia on plateaus. However, its mechanism of action is not fully defined. In this study, we investigate the mechanism by which B. longum intervenes in intestinal barrier damage caused by plateau low-pressure hypoxia. To this end, an in vivo model is established by exposing rats to a simulated low-pressure hypoxic plateau environment. The experimental rats were subsequently supplemented with a B. longum strain (JBLC-141) extracted from the feces of healthy adults in Bama, Guangxi. B. longum JBLC-141 mitigates the effects of plateau low-pressure hypoxia on the rat intestinal barrier. This is achieved by activating the intestinal Kelch-like ECH-associated protein 1 (KEAP1)/nuclear factor erythroid 2-related factor 2 (NRF2) pathway, alleviating plateau hypoxia-induced intestinal oxidative stress injury. B. longum JBLC-141 also attenuates the inflammatory response and upregulates the expression of the tight junction proteins claudin-1, occludin, and zonula occludens-1. Furthermore, it reduces intestinal permeability, effectively ameliorating and repairing the barrier histological damage induced by the plateau low-pressure hypoxic environment. In addition, B. longum JBLC-141 positively regulates the intestinal microbiota, increasing the relative abundance of beneficial bacteria while reducing that of pathogenic bacteria and maintaining intestinal flora homeostasis in rats. |
| format | Article |
| id | doaj-art-02376a615a114ec5b059eebc68c54afd |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-02376a615a114ec5b059eebc68c54afd2025-08-20T01:57:49ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-12-011510.3389/fmicb.2024.15019991501999Bifidobacterium longum JBLC-141 alleviates hypobaric hypoxia-induced intestinal barrier damage by attenuating inflammatory responses and oxidative stressXiang-Yang Li0Xiang-Yang Li1Xiang-Yang Li2Jin Shang3Jin Shang4Jin Shang5Xiao-Juan Wang6Xiao-Juan Wang7Xiao-Juan Wang8Hui-Ping Ma9Long-Fei Ren10Long-Fei Ren11Long-Fei Ren12Long-Fei Ren13Long-Fei Ren14Lei Zhang15Lei Zhang16Lei Zhang17Lei Zhang18Lei Zhang19The First Clinical Medical College, Lanzhou University, Lanzhou, Gansu, ChinaDepartment of General Surgery, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaLaboratory of Biological Therapy and Regenerative Medicine Transformation Gansu Province, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaThe First Clinical Medical College, Lanzhou University, Lanzhou, Gansu, ChinaDepartment of General Surgery, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaLaboratory of Biological Therapy and Regenerative Medicine Transformation Gansu Province, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaThe First Clinical Medical College, Lanzhou University, Lanzhou, Gansu, ChinaDepartment of General Surgery, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaLaboratory of Biological Therapy and Regenerative Medicine Transformation Gansu Province, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaPharmacy Department, The 940 Hospital of Joint Logistics Support, PLA, Lanzhou, Gansu, ChinaThe First Clinical Medical College, Lanzhou University, Lanzhou, Gansu, ChinaDepartment of General Surgery, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaLaboratory of Biological Therapy and Regenerative Medicine Transformation Gansu Province, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaNational Clinical key Specialty of General Surgery, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaClinical Research Center for General Surgery of Gansu Province, Lanzhou, Gansu, ChinaThe First Clinical Medical College, Lanzhou University, Lanzhou, Gansu, ChinaDepartment of General Surgery, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaLaboratory of Biological Therapy and Regenerative Medicine Transformation Gansu Province, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaNational Clinical key Specialty of General Surgery, The First Hospital of Lanzhou University, Lanzhou, Gansu, ChinaClinical Research Center for General Surgery of Gansu Province, Lanzhou, Gansu, ChinaHypobaric hypoxia exposure occurs at high altitudes, including plateaus, and affects normal intestinal function and microbiota composition. Exposure induces an intestinal inflammatory response and oxidative stress injury, ultimately disrupting intestinal homeostasis and causing barrier damage. Thus, due to its anti-inflammatory, antioxidative, and intestinal microbiota-regulating properties, Bifidobacterium longum is a potentially effective probiotic intervention to protect the intestinal barrier during low-pressure hypoxia on plateaus. However, its mechanism of action is not fully defined. In this study, we investigate the mechanism by which B. longum intervenes in intestinal barrier damage caused by plateau low-pressure hypoxia. To this end, an in vivo model is established by exposing rats to a simulated low-pressure hypoxic plateau environment. The experimental rats were subsequently supplemented with a B. longum strain (JBLC-141) extracted from the feces of healthy adults in Bama, Guangxi. B. longum JBLC-141 mitigates the effects of plateau low-pressure hypoxia on the rat intestinal barrier. This is achieved by activating the intestinal Kelch-like ECH-associated protein 1 (KEAP1)/nuclear factor erythroid 2-related factor 2 (NRF2) pathway, alleviating plateau hypoxia-induced intestinal oxidative stress injury. B. longum JBLC-141 also attenuates the inflammatory response and upregulates the expression of the tight junction proteins claudin-1, occludin, and zonula occludens-1. Furthermore, it reduces intestinal permeability, effectively ameliorating and repairing the barrier histological damage induced by the plateau low-pressure hypoxic environment. In addition, B. longum JBLC-141 positively regulates the intestinal microbiota, increasing the relative abundance of beneficial bacteria while reducing that of pathogenic bacteria and maintaining intestinal flora homeostasis in rats.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1501999/fullBifidobacterium longumhypobaric hypoxiaoxidative stressintestinal barrierintestinal flora |
| spellingShingle | Xiang-Yang Li Xiang-Yang Li Xiang-Yang Li Jin Shang Jin Shang Jin Shang Xiao-Juan Wang Xiao-Juan Wang Xiao-Juan Wang Hui-Ping Ma Long-Fei Ren Long-Fei Ren Long-Fei Ren Long-Fei Ren Long-Fei Ren Lei Zhang Lei Zhang Lei Zhang Lei Zhang Lei Zhang Bifidobacterium longum JBLC-141 alleviates hypobaric hypoxia-induced intestinal barrier damage by attenuating inflammatory responses and oxidative stress Frontiers in Microbiology Bifidobacterium longum hypobaric hypoxia oxidative stress intestinal barrier intestinal flora |
| title | Bifidobacterium longum JBLC-141 alleviates hypobaric hypoxia-induced intestinal barrier damage by attenuating inflammatory responses and oxidative stress |
| title_full | Bifidobacterium longum JBLC-141 alleviates hypobaric hypoxia-induced intestinal barrier damage by attenuating inflammatory responses and oxidative stress |
| title_fullStr | Bifidobacterium longum JBLC-141 alleviates hypobaric hypoxia-induced intestinal barrier damage by attenuating inflammatory responses and oxidative stress |
| title_full_unstemmed | Bifidobacterium longum JBLC-141 alleviates hypobaric hypoxia-induced intestinal barrier damage by attenuating inflammatory responses and oxidative stress |
| title_short | Bifidobacterium longum JBLC-141 alleviates hypobaric hypoxia-induced intestinal barrier damage by attenuating inflammatory responses and oxidative stress |
| title_sort | bifidobacterium longum jblc 141 alleviates hypobaric hypoxia induced intestinal barrier damage by attenuating inflammatory responses and oxidative stress |
| topic | Bifidobacterium longum hypobaric hypoxia oxidative stress intestinal barrier intestinal flora |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1501999/full |
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