Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes Patients
A possible role of Snap25 polymorphisms in type 2 diabetes mellitus (T2DM) was evaluated by analyzing three SNPs within intron 1 in a region known to affect the gene expression in vitro. Genomic DNA from 1019 Saudi individuals (489 confirmed T2DM and 530 controls) was genotyped for SNPs rs363039, rs...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/8943092 |
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| author | Nasser M. Al-Daghri Andrea S. Costa Majed S. Alokail Milena Zanzottera Amal M. Alenad Abdul Khader Mohammed Mario Clerici Franca R. Guerini |
| author_facet | Nasser M. Al-Daghri Andrea S. Costa Majed S. Alokail Milena Zanzottera Amal M. Alenad Abdul Khader Mohammed Mario Clerici Franca R. Guerini |
| author_sort | Nasser M. Al-Daghri |
| collection | DOAJ |
| description | A possible role of Snap25 polymorphisms in type 2 diabetes mellitus (T2DM) was evaluated by analyzing three SNPs within intron 1 in a region known to affect the gene expression in vitro. Genomic DNA from 1019 Saudi individuals (489 confirmed T2DM and 530 controls) was genotyped for SNPs rs363039, rs363043, and rs363050 in Snap25 using the TaqMan Genotyping Assay. Significantly higher levels of fasting glucose and HbA1c were detected in T2DM patients carrying the rs363050 (AG/GG) genotypes compared to the (AA) genotype (f=4.41, df=1, and p=0.03 and f=5.31, df=1, and p=0.03, resp.). In these same patients, insulin levels were significantly decreased compared to the (AA) individuals (f=7.29, df=1, and p=0.009). Significant associations were detected between rs363050 (AG/GG) genotypes and increasing fasting glucose levels (p=0.01 and OR: 1.05), HbA1c levels (OR: 5.06 and p=0.02), and lower insulinemia (p=0.03 and OR: 0.95) in T2DM patients. The minor Snap25 rs363050 (G) allele, which results in a reduced expression of Snap25, is associated with altered glycemic parameters in T2DM possibly because of reduced functionality in the exocytotic machinery leading to suboptimal release of insulin. |
| format | Article |
| id | doaj-art-0234aaf0990340c2aba1a1db166a37f6 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-0234aaf0990340c2aba1a1db166a37f62025-02-03T01:22:59ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/89430928943092Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes PatientsNasser M. Al-Daghri0Andrea S. Costa1Majed S. Alokail2Milena Zanzottera3Amal M. Alenad4Abdul Khader Mohammed5Mario Clerici6Franca R. Guerini7Biomarkers Research Program, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaFondazione Don C Gnocchi, IRCCS, 20148 Milano, ItalyBiomarkers Research Program, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaFondazione Don C Gnocchi, IRCCS, 20148 Milano, ItalySchool of Biological Sciences, University of Southampton, Southampton SO17 1 BJ, UKBiomarkers Research Program, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaFondazione Don C Gnocchi, IRCCS, 20148 Milano, ItalyFondazione Don C Gnocchi, IRCCS, 20148 Milano, ItalyA possible role of Snap25 polymorphisms in type 2 diabetes mellitus (T2DM) was evaluated by analyzing three SNPs within intron 1 in a region known to affect the gene expression in vitro. Genomic DNA from 1019 Saudi individuals (489 confirmed T2DM and 530 controls) was genotyped for SNPs rs363039, rs363043, and rs363050 in Snap25 using the TaqMan Genotyping Assay. Significantly higher levels of fasting glucose and HbA1c were detected in T2DM patients carrying the rs363050 (AG/GG) genotypes compared to the (AA) genotype (f=4.41, df=1, and p=0.03 and f=5.31, df=1, and p=0.03, resp.). In these same patients, insulin levels were significantly decreased compared to the (AA) individuals (f=7.29, df=1, and p=0.009). Significant associations were detected between rs363050 (AG/GG) genotypes and increasing fasting glucose levels (p=0.01 and OR: 1.05), HbA1c levels (OR: 5.06 and p=0.02), and lower insulinemia (p=0.03 and OR: 0.95) in T2DM patients. The minor Snap25 rs363050 (G) allele, which results in a reduced expression of Snap25, is associated with altered glycemic parameters in T2DM possibly because of reduced functionality in the exocytotic machinery leading to suboptimal release of insulin.http://dx.doi.org/10.1155/2016/8943092 |
| spellingShingle | Nasser M. Al-Daghri Andrea S. Costa Majed S. Alokail Milena Zanzottera Amal M. Alenad Abdul Khader Mohammed Mario Clerici Franca R. Guerini Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes Patients Journal of Diabetes Research |
| title | Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes Patients |
| title_full | Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes Patients |
| title_fullStr | Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes Patients |
| title_full_unstemmed | Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes Patients |
| title_short | Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes Patients |
| title_sort | synaptosomal protein of 25 kda snap25 polymorphisms associated with glycemic parameters in type 2 diabetes patients |
| url | http://dx.doi.org/10.1155/2016/8943092 |
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