Characterization and genomic analysis of two Aeromonas phages
Phages, as viruses that specifically infect certain bacteria, have great research value in the prevention and control of bacterial diseases. Bacteria of the genus Aeromonas are widely distributed in aquatic environments and are important zoonotic pathogens that can cause a variety of diseases. In th...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1585026/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849333534961958912 |
|---|---|
| author | Li ling Jiang Li ling Jiang Chang Liu Guang feng Liu Xiu hua Tao Hong Sai Zhang Yue Zhang Yuyu Chen Meng yao Li Jing zhe Jiang Hong peng Chen |
| author_facet | Li ling Jiang Li ling Jiang Chang Liu Guang feng Liu Xiu hua Tao Hong Sai Zhang Yue Zhang Yuyu Chen Meng yao Li Jing zhe Jiang Hong peng Chen |
| author_sort | Li ling Jiang |
| collection | DOAJ |
| description | Phages, as viruses that specifically infect certain bacteria, have great research value in the prevention and control of bacterial diseases. Bacteria of the genus Aeromonas are widely distributed in aquatic environments and are important zoonotic pathogens that can cause a variety of diseases. In this study, two virulent phages, AhC3_1 and AsC4_1, were successfully isolated using bacteria from the genus Aeromonas. The evolutionary relationships of the two phages were analyzed using methods such as phylogenetic analysis and average amino acid identity (AAI) analysis, and their biological characteristics were assessed through approaches including one-step growth curve assay and temperature tolerance testing. The results showed that the genome sequences of phages C3 and C4 were 232,884 bp and 45,983 bp in length, respectively, with G + C contents of 44.36% and 50.11%. Both belong to double-stranded circular DNA viruses. Phages C3 and C4 have been classified within two novel viral genera, provisionally designated as “Aynavirus” and “Asnavirus,” respectively. Additionally, both C3 and C4 exhibited good stability in different temperature and pH conditions. The latent period of C3 was 10 min, with a burst size of 32 PFU.cell−1. The in vitro antibacterial curve showed that when the multiplicity of infection (MOI) was 10−3, C3 could exert a strong inhibitory effect on Aeromonas hydrophila for up to 10 h. The latent period of C4 was less than 10 min, with a burst size of 1,250 PFU.cell−1. At an MOI of 10−1, C4 could exert a strong inhibitory effect on Aeromonas salmonicida for up to 15 h. In summary, the two phages isolated in this study have the potential to serve as potential therapeutic agents for aquaculture diseases and provide valuable data for research on antibacterial infections. |
| format | Article |
| id | doaj-art-020d55e331bf450684cc7c0032267bdc |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-020d55e331bf450684cc7c0032267bdc2025-08-20T03:45:49ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-06-011610.3389/fmicb.2025.15850261585026Characterization and genomic analysis of two Aeromonas phagesLi ling Jiang0Li ling Jiang1Chang Liu2Guang feng Liu3Xiu hua Tao4Hong Sai Zhang5Yue Zhang6Yuyu Chen7Meng yao Li8Jing zhe Jiang9Hong peng Chen10School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, ChinaInstitute of Forest Medicinal Materials and Food, Jiangxi Academy of Forestry, Nanchang, Jiangxi, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong, ChinaPhages, as viruses that specifically infect certain bacteria, have great research value in the prevention and control of bacterial diseases. Bacteria of the genus Aeromonas are widely distributed in aquatic environments and are important zoonotic pathogens that can cause a variety of diseases. In this study, two virulent phages, AhC3_1 and AsC4_1, were successfully isolated using bacteria from the genus Aeromonas. The evolutionary relationships of the two phages were analyzed using methods such as phylogenetic analysis and average amino acid identity (AAI) analysis, and their biological characteristics were assessed through approaches including one-step growth curve assay and temperature tolerance testing. The results showed that the genome sequences of phages C3 and C4 were 232,884 bp and 45,983 bp in length, respectively, with G + C contents of 44.36% and 50.11%. Both belong to double-stranded circular DNA viruses. Phages C3 and C4 have been classified within two novel viral genera, provisionally designated as “Aynavirus” and “Asnavirus,” respectively. Additionally, both C3 and C4 exhibited good stability in different temperature and pH conditions. The latent period of C3 was 10 min, with a burst size of 32 PFU.cell−1. The in vitro antibacterial curve showed that when the multiplicity of infection (MOI) was 10−3, C3 could exert a strong inhibitory effect on Aeromonas hydrophila for up to 10 h. The latent period of C4 was less than 10 min, with a burst size of 1,250 PFU.cell−1. At an MOI of 10−1, C4 could exert a strong inhibitory effect on Aeromonas salmonicida for up to 15 h. In summary, the two phages isolated in this study have the potential to serve as potential therapeutic agents for aquaculture diseases and provide valuable data for research on antibacterial infections.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1585026/fullAeromonas phagesbiological characteristicsgenomic analysisphylogenetic treeantibacterial curve |
| spellingShingle | Li ling Jiang Li ling Jiang Chang Liu Guang feng Liu Xiu hua Tao Hong Sai Zhang Yue Zhang Yuyu Chen Meng yao Li Jing zhe Jiang Hong peng Chen Characterization and genomic analysis of two Aeromonas phages Frontiers in Microbiology Aeromonas phages biological characteristics genomic analysis phylogenetic tree antibacterial curve |
| title | Characterization and genomic analysis of two Aeromonas phages |
| title_full | Characterization and genomic analysis of two Aeromonas phages |
| title_fullStr | Characterization and genomic analysis of two Aeromonas phages |
| title_full_unstemmed | Characterization and genomic analysis of two Aeromonas phages |
| title_short | Characterization and genomic analysis of two Aeromonas phages |
| title_sort | characterization and genomic analysis of two aeromonas phages |
| topic | Aeromonas phages biological characteristics genomic analysis phylogenetic tree antibacterial curve |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1585026/full |
| work_keys_str_mv | AT lilingjiang characterizationandgenomicanalysisoftwoaeromonasphages AT lilingjiang characterizationandgenomicanalysisoftwoaeromonasphages AT changliu characterizationandgenomicanalysisoftwoaeromonasphages AT guangfengliu characterizationandgenomicanalysisoftwoaeromonasphages AT xiuhuatao characterizationandgenomicanalysisoftwoaeromonasphages AT hongsaizhang characterizationandgenomicanalysisoftwoaeromonasphages AT yuezhang characterizationandgenomicanalysisoftwoaeromonasphages AT yuyuchen characterizationandgenomicanalysisoftwoaeromonasphages AT mengyaoli characterizationandgenomicanalysisoftwoaeromonasphages AT jingzhejiang characterizationandgenomicanalysisoftwoaeromonasphages AT hongpengchen characterizationandgenomicanalysisoftwoaeromonasphages |