Snhg3 improves glucose metabolism by promoting Sestrin2 expression in mice
Objective To investigate the role of long non-coding RNA-small nucleolar RNA host gene 3(lncRNA-Snhg3) and its regulatory mechanism in the hepatic glucose metabolism of mice. Methods Adenovirus Snhg3 was over-expressed by the tail vein injection in db/db mice, and then glucose tolerance and pyruvate...
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-06-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-714.pdf |
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| author | ZHANG Minglong, GAO Mingyue, XIE Xianghong, GUO Zeyu, LIU Xiaojun, YAN Li |
| author_facet | ZHANG Minglong, GAO Mingyue, XIE Xianghong, GUO Zeyu, LIU Xiaojun, YAN Li |
| author_sort | ZHANG Minglong, GAO Mingyue, XIE Xianghong, GUO Zeyu, LIU Xiaojun, YAN Li |
| collection | DOAJ |
| description | Objective To investigate the role of long non-coding RNA-small nucleolar RNA host gene 3(lncRNA-Snhg3) and its regulatory mechanism in the hepatic glucose metabolism of mice. Methods Adenovirus Snhg3 was over-expressed by the tail vein injection in db/db mice, and then glucose tolerance and pyruvate tolerance were measured. The mRNA expression of mouse liver gluconeogenesis-related genes phosphoenolpyruvate carboxylase(Pepck) and glucose-6-phosphatase(G6pc) and stress-inducing protein 2(Sestrin2,Sesn2,a gene adjacent to Snhg3) were detected by RT-qPCR. The dual luciferase reporter assay was used to detect the effect of Snhg3 on the Sesn2 promoter activity in 293T cells. Results Snhg3 over-expression improved glucose tolerance and pyruvate tolerance in db/db mice. Snhg3 over-expression inhibited the mRNA of gluconeogenesis genes of Pepck(P＜0.05) and G6pc(P＜0.05), while promoted the mRNA of Sesn2(P＜0.01). Meanwhile,Snhg3 over-expression promoted Sesn2 promoter activity in 293T cells(P＜0.05). Conclusions Snhg3 improves glucose metabolism in mice by promoting Sestrin2 expression. |
| format | Article |
| id | doaj-art-020b75b5cc0e4ebaaaea1123f32d4e0a |
| institution | DOAJ |
| issn | 1001-6325 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj-art-020b75b5cc0e4ebaaaea1123f32d4e0a2025-08-20T03:14:49ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252025-06-0145671471910.16352/j.issn.1001-6325.2025.06.0714Snhg3 improves glucose metabolism by promoting Sestrin2 expression in miceZHANG Minglong, GAO Mingyue, XIE Xianghong, GUO Zeyu, LIU Xiaojun, YAN Li01. Department of Pathophysiology; 2. Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, ChinaObjective To investigate the role of long non-coding RNA-small nucleolar RNA host gene 3(lncRNA-Snhg3) and its regulatory mechanism in the hepatic glucose metabolism of mice. Methods Adenovirus Snhg3 was over-expressed by the tail vein injection in db/db mice, and then glucose tolerance and pyruvate tolerance were measured. The mRNA expression of mouse liver gluconeogenesis-related genes phosphoenolpyruvate carboxylase(Pepck) and glucose-6-phosphatase(G6pc) and stress-inducing protein 2(Sestrin2,Sesn2,a gene adjacent to Snhg3) were detected by RT-qPCR. The dual luciferase reporter assay was used to detect the effect of Snhg3 on the Sesn2 promoter activity in 293T cells. Results Snhg3 over-expression improved glucose tolerance and pyruvate tolerance in db/db mice. Snhg3 over-expression inhibited the mRNA of gluconeogenesis genes of Pepck(P＜0.05) and G6pc(P＜0.05), while promoted the mRNA of Sesn2(P＜0.01). Meanwhile,Snhg3 over-expression promoted Sesn2 promoter activity in 293T cells(P＜0.05). Conclusions Snhg3 improves glucose metabolism in mice by promoting Sestrin2 expression.https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-714.pdfsmall nucleolar rna host gene 3(snhg3)|hepatic gluconeogenesis|stress-inducing protein 2(sestrin 2) |
| spellingShingle | ZHANG Minglong, GAO Mingyue, XIE Xianghong, GUO Zeyu, LIU Xiaojun, YAN Li Snhg3 improves glucose metabolism by promoting Sestrin2 expression in mice Jichu yixue yu linchuang small nucleolar rna host gene 3(snhg3)|hepatic gluconeogenesis|stress-inducing protein 2(sestrin 2) |
| title | Snhg3 improves glucose metabolism by promoting Sestrin2 expression in mice |
| title_full | Snhg3 improves glucose metabolism by promoting Sestrin2 expression in mice |
| title_fullStr | Snhg3 improves glucose metabolism by promoting Sestrin2 expression in mice |
| title_full_unstemmed | Snhg3 improves glucose metabolism by promoting Sestrin2 expression in mice |
| title_short | Snhg3 improves glucose metabolism by promoting Sestrin2 expression in mice |
| title_sort | snhg3 improves glucose metabolism by promoting sestrin2 expression in mice |
| topic | small nucleolar rna host gene 3(snhg3)|hepatic gluconeogenesis|stress-inducing protein 2(sestrin 2) |
| url | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-714.pdf |
| work_keys_str_mv | AT zhangminglonggaomingyuexiexianghongguozeyuliuxiaojunyanli snhg3improvesglucosemetabolismbypromotingsestrin2expressioninmice |