An Investigation of Polymorphism on the FASN, SCD1, and SLC27A3 Genes in Sheep
Milk traits of sheep are affected by many environmental and genetic factors. These traits are quantitative traits and are determined by many genes. This study aimed to investigate polymorphisms of milk-related Fatty-acid synthase (FASN), Stearoyl-CoA desaturase (SCD1), and solute carrier 27A3 (SLC27...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hasan Eleroğlu
2024-10-01
|
| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | https://agrifoodscience.com/index.php/TURJAF/article/view/7047 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849687033638813696 |
|---|---|
| author | Ali Osman Turgut Erman Gülendağ Mürsel Küçük Davut Koca Adnan Ayan Mehmet Eroğlu Muhammed Furkan Önen |
| author_facet | Ali Osman Turgut Erman Gülendağ Mürsel Küçük Davut Koca Adnan Ayan Mehmet Eroğlu Muhammed Furkan Önen |
| author_sort | Ali Osman Turgut |
| collection | DOAJ |
| description | Milk traits of sheep are affected by many environmental and genetic factors. These traits are quantitative traits and are determined by many genes. This study aimed to investigate polymorphisms of milk-related Fatty-acid synthase (FASN), Stearoyl-CoA desaturase (SCD1), and solute carrier 27A3 (SLC27A3) genes in cross-bred Hamdani sheep. Blood samples were collected from 100 healthy cross-bred Hamdani ewes from the jugular vein into K3-EDTA-containing tubes. Genomic DNA was extracted using a commercial DNA isolation kit. PCR products of FASN (275 bp), SCD1 (225 bp), SLC27A3-P1 (341 bp), and SLC27A3-P2 (319 bp) were subjected to restriction fragment length polymorphism (RFLP) analysis using SSil (AciI), Cfr13I, AluI, and Hpy188III the restriction enzyme, respectively. However, all gene regions were found to be monomorphic. In the study, only TT, AA, AA, and GG genotypes were detected for FASN, SCD1, SLC27A3-P1, and SLC27A3-P2, respectively. Allele and genotype frequencies were 1.00 for all genotypes and alleles. Although this study did not reveal favorable genotypes in FASN, SCD1, and SLC27A3 genes that can be used for milk traits, more comprehensive studies with larger sample sizes should be conducted to investigate polymorphisms in cross-bred Hamdani sheep. |
| format | Article |
| id | doaj-art-02092b59a6034d45ace2b9ecbfe2c9ca |
| institution | DOAJ |
| issn | 2148-127X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-02092b59a6034d45ace2b9ecbfe2c9ca2025-08-20T03:22:27ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2024-10-0112101812181610.24925/turjaf.v12i10.1812-1816.70475748An Investigation of Polymorphism on the FASN, SCD1, and SLC27A3 Genes in SheepAli Osman Turgut0https://orcid.org/0000-0001-6863-0939Erman Gülendağ1https://orcid.org/0000-0002-3335-7247Mürsel Küçük2https://orcid.org/0000-0002-0544-444XDavut Koca3https://orcid.org/0000-0002-7962-6959Adnan Ayan4https://orcid.org/0000-0002-6564-3416Mehmet Eroğlu5https://orcid.org/0000-0001-9471-6410Muhammed Furkan Önen6https://orcid.org/0009-0000-4481-5891Department of Animal Science, Faculty of Veterinary Medicine, Siirt University, Siirt, TürkiyeDepartment of Biostatistics, Faculty of Veterinary Medicine, Siirt University, Siirt, TürkiyeDepartment of Animal Science, Faculty of Veterinary Medicine, Siirt University, Siirt, TürkiyeDepartment of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Yuzuncu Yil University, Van, TürkiyeDepartment of Genetics, Faculty of Veterinary Medicine, Yuzuncu Yil University, Van, TürkiyeDepartment of Animal Science, Faculty of Veterinary Medicine, Siirt University, Siirt, TürkiyeDepartment of Animal Science, Faculty of Veterinary Medicine, Siirt University, Siirt, TürkiyeMilk traits of sheep are affected by many environmental and genetic factors. These traits are quantitative traits and are determined by many genes. This study aimed to investigate polymorphisms of milk-related Fatty-acid synthase (FASN), Stearoyl-CoA desaturase (SCD1), and solute carrier 27A3 (SLC27A3) genes in cross-bred Hamdani sheep. Blood samples were collected from 100 healthy cross-bred Hamdani ewes from the jugular vein into K3-EDTA-containing tubes. Genomic DNA was extracted using a commercial DNA isolation kit. PCR products of FASN (275 bp), SCD1 (225 bp), SLC27A3-P1 (341 bp), and SLC27A3-P2 (319 bp) were subjected to restriction fragment length polymorphism (RFLP) analysis using SSil (AciI), Cfr13I, AluI, and Hpy188III the restriction enzyme, respectively. However, all gene regions were found to be monomorphic. In the study, only TT, AA, AA, and GG genotypes were detected for FASN, SCD1, SLC27A3-P1, and SLC27A3-P2, respectively. Allele and genotype frequencies were 1.00 for all genotypes and alleles. Although this study did not reveal favorable genotypes in FASN, SCD1, and SLC27A3 genes that can be used for milk traits, more comprehensive studies with larger sample sizes should be conducted to investigate polymorphisms in cross-bred Hamdani sheep.https://agrifoodscience.com/index.php/TURJAF/article/view/7047cross-bred hamdani sheepgene polymorphismpcr-rflpmilkproduction |
| spellingShingle | Ali Osman Turgut Erman Gülendağ Mürsel Küçük Davut Koca Adnan Ayan Mehmet Eroğlu Muhammed Furkan Önen An Investigation of Polymorphism on the FASN, SCD1, and SLC27A3 Genes in Sheep Turkish Journal of Agriculture: Food Science and Technology cross-bred hamdani sheep gene polymorphism pcr-rflp milk production |
| title | An Investigation of Polymorphism on the FASN, SCD1, and SLC27A3 Genes in Sheep |
| title_full | An Investigation of Polymorphism on the FASN, SCD1, and SLC27A3 Genes in Sheep |
| title_fullStr | An Investigation of Polymorphism on the FASN, SCD1, and SLC27A3 Genes in Sheep |
| title_full_unstemmed | An Investigation of Polymorphism on the FASN, SCD1, and SLC27A3 Genes in Sheep |
| title_short | An Investigation of Polymorphism on the FASN, SCD1, and SLC27A3 Genes in Sheep |
| title_sort | investigation of polymorphism on the fasn scd1 and slc27a3 genes in sheep |
| topic | cross-bred hamdani sheep gene polymorphism pcr-rflp milk production |
| url | https://agrifoodscience.com/index.php/TURJAF/article/view/7047 |
| work_keys_str_mv | AT aliosmanturgut aninvestigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT ermangulendag aninvestigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT murselkucuk aninvestigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT davutkoca aninvestigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT adnanayan aninvestigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT mehmeteroglu aninvestigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT muhammedfurkanonen aninvestigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT aliosmanturgut investigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT ermangulendag investigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT murselkucuk investigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT davutkoca investigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT adnanayan investigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT mehmeteroglu investigationofpolymorphismonthefasnscd1andslc27a3genesinsheep AT muhammedfurkanonen investigationofpolymorphismonthefasnscd1andslc27a3genesinsheep |