Sensitive and rapid detection of bisphenol A using signal amplification nanoparticles loaded with anti-bisphenol A monoclonal antibody
Bisphenol A has been reported to be a ubiquitous contaminant, and exposure to this compound can lead to adverse effects in human health. In the study, monoclonal antibody against BPA (anti-BPA mAb) with high affinity (3.74 × 109 L/mol) secreted by cell line 2E3 was successfully screened. Inspired by...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524007910 |
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| author | Sumei Ling Aidi Xu Menghan Sun Xiaoli Li Yongming Huang Yang Xu Jianli Huang Tingting Xie Shihua Wang |
| author_facet | Sumei Ling Aidi Xu Menghan Sun Xiaoli Li Yongming Huang Yang Xu Jianli Huang Tingting Xie Shihua Wang |
| author_sort | Sumei Ling |
| collection | DOAJ |
| description | Bisphenol A has been reported to be a ubiquitous contaminant, and exposure to this compound can lead to adverse effects in human health. In the study, monoclonal antibody against BPA (anti-BPA mAb) with high affinity (3.74 × 109 L/mol) secreted by cell line 2E3 was successfully screened. Inspired by the signal amplification of nanoparticles, anti-BPA mAbs were labeled with nano-materials including colloidal gold (AuNP) and gold nanoflowers (AuNF) for preparation of immunoprobes and AuNP-/AuNF-based test strips. The developed AuNP- and AuNF-based test strips achieved the rapid and sensitive detection of BPA within 10 min, with the limit of detection (LOD) of 25 μg/mL and 3.125 μg/mL, respectively. The detection result in BPA spiked samples measured by the proposed methods was consistent with that detected by LC-MS method. The preparation process of as-prepared test strip is time-saving and considered as ideal candidates method for rapid screening BPA in real samples. |
| format | Article |
| id | doaj-art-01fc0e1092f24e5bbbf23c1d5bfd856b |
| institution | DOAJ |
| issn | 2590-1575 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-01fc0e1092f24e5bbbf23c1d5bfd856b2025-08-20T02:49:55ZengElsevierFood Chemistry: X2590-15752024-12-012410190310.1016/j.fochx.2024.101903Sensitive and rapid detection of bisphenol A using signal amplification nanoparticles loaded with anti-bisphenol A monoclonal antibodySumei Ling0Aidi Xu1Menghan Sun2Xiaoli Li3Yongming Huang4Yang Xu5Jianli Huang6Tingting Xie7Shihua Wang8State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, the Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, the Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, the Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, the Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Grain and Oil Quality Supervision and Test of Fujian Province, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, the Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, the Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Grain and Oil Quality Supervision and Test of Fujian Province, Fuzhou 350002, ChinaInstitute of Grain and Oil Quality Supervision and Test of Fujian Province, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, the Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Corresponding author.Bisphenol A has been reported to be a ubiquitous contaminant, and exposure to this compound can lead to adverse effects in human health. In the study, monoclonal antibody against BPA (anti-BPA mAb) with high affinity (3.74 × 109 L/mol) secreted by cell line 2E3 was successfully screened. Inspired by the signal amplification of nanoparticles, anti-BPA mAbs were labeled with nano-materials including colloidal gold (AuNP) and gold nanoflowers (AuNF) for preparation of immunoprobes and AuNP-/AuNF-based test strips. The developed AuNP- and AuNF-based test strips achieved the rapid and sensitive detection of BPA within 10 min, with the limit of detection (LOD) of 25 μg/mL and 3.125 μg/mL, respectively. The detection result in BPA spiked samples measured by the proposed methods was consistent with that detected by LC-MS method. The preparation process of as-prepared test strip is time-saving and considered as ideal candidates method for rapid screening BPA in real samples.http://www.sciencedirect.com/science/article/pii/S2590157524007910Bisphenol AImmunochromatographic assayMonoclonal antibodyNanoparticleImmunoprobe |
| spellingShingle | Sumei Ling Aidi Xu Menghan Sun Xiaoli Li Yongming Huang Yang Xu Jianli Huang Tingting Xie Shihua Wang Sensitive and rapid detection of bisphenol A using signal amplification nanoparticles loaded with anti-bisphenol A monoclonal antibody Food Chemistry: X Bisphenol A Immunochromatographic assay Monoclonal antibody Nanoparticle Immunoprobe |
| title | Sensitive and rapid detection of bisphenol A using signal amplification nanoparticles loaded with anti-bisphenol A monoclonal antibody |
| title_full | Sensitive and rapid detection of bisphenol A using signal amplification nanoparticles loaded with anti-bisphenol A monoclonal antibody |
| title_fullStr | Sensitive and rapid detection of bisphenol A using signal amplification nanoparticles loaded with anti-bisphenol A monoclonal antibody |
| title_full_unstemmed | Sensitive and rapid detection of bisphenol A using signal amplification nanoparticles loaded with anti-bisphenol A monoclonal antibody |
| title_short | Sensitive and rapid detection of bisphenol A using signal amplification nanoparticles loaded with anti-bisphenol A monoclonal antibody |
| title_sort | sensitive and rapid detection of bisphenol a using signal amplification nanoparticles loaded with anti bisphenol a monoclonal antibody |
| topic | Bisphenol A Immunochromatographic assay Monoclonal antibody Nanoparticle Immunoprobe |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524007910 |
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