Determination of Glucocorticoids in UPLC-MS in Environmental Samples from an Occupational Setting
Occupational exposures to glucocorticoids are still a neglected issue in some work environments, including pharmaceutical plants. We developed an analytical method to quantify simultaneously 21 glucocorticoids using UPLC coupled with mass spectrometry to provide a basis to carry out environmental mo...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/678579 |
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| author | Enrico Oddone Sara Negri Massimo Bellinzona Silvia Martino Marcello Di Tuccio Elena Grignani Danilo Cottica Marcello Imbriani |
| author_facet | Enrico Oddone Sara Negri Massimo Bellinzona Silvia Martino Marcello Di Tuccio Elena Grignani Danilo Cottica Marcello Imbriani |
| author_sort | Enrico Oddone |
| collection | DOAJ |
| description | Occupational exposures to glucocorticoids are still a neglected issue in some work environments, including pharmaceutical plants. We developed an analytical method to quantify simultaneously 21 glucocorticoids using UPLC coupled with mass spectrometry to provide a basis to carry out environmental monitoring. Samples were taken from air, hand-washing tests, pad-tests and wipe-tests. This paper reports the contents of the analytical methodology, along with the results of this extensive environmental and personal monitoring of glucocorticoids. The method in UPLC-MS turned out to be suitable and effective for the aim of the study. Wipe-test and pad-test desorption was carried out using 50 mL syringes, a simple technique that saves time without adversely affecting analyte recovery. Results showed a widespread environmental pollution due to glucocorticoids. This is of particular concern. Evaluation of the dose absorbed by each worker and identification of a biomarker for occupational exposure will contribute to assessment and prevention of occupational exposure. |
| format | Article |
| id | doaj-art-01f9560524334f17857c1768b868c0ef |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-01f9560524334f17857c1768b868c0ef2025-02-03T01:11:12ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792015-01-01201510.1155/2015/678579678579Determination of Glucocorticoids in UPLC-MS in Environmental Samples from an Occupational SettingEnrico Oddone0Sara Negri1Massimo Bellinzona2Silvia Martino3Marcello Di Tuccio4Elena Grignani5Danilo Cottica6Marcello Imbriani7Department of Public Health, Experimental and Forensic Medicine, University of Pavia, 27100 Pavia, ItalyEnvironmental Research Center, Salvatore Maugeri Foundation, Via S. Maugeri 10, 27100 Pavia, ItalyEnvironmental Research Center, Salvatore Maugeri Foundation, Via S. Maugeri 10, 27100 Pavia, ItalyEnvironmental Research Center, Salvatore Maugeri Foundation, Via S. Maugeri 10, 27100 Pavia, ItalyEnvironmental Research Center, Salvatore Maugeri Foundation, Via S. Maugeri 10, 27100 Pavia, ItalyEnvironmental Research Center, Salvatore Maugeri Foundation, Via S. Maugeri 10, 27100 Pavia, ItalyEnvironmental Research Center, Salvatore Maugeri Foundation, Via S. Maugeri 10, 27100 Pavia, ItalyDepartment of Public Health, Experimental and Forensic Medicine, University of Pavia, 27100 Pavia, ItalyOccupational exposures to glucocorticoids are still a neglected issue in some work environments, including pharmaceutical plants. We developed an analytical method to quantify simultaneously 21 glucocorticoids using UPLC coupled with mass spectrometry to provide a basis to carry out environmental monitoring. Samples were taken from air, hand-washing tests, pad-tests and wipe-tests. This paper reports the contents of the analytical methodology, along with the results of this extensive environmental and personal monitoring of glucocorticoids. The method in UPLC-MS turned out to be suitable and effective for the aim of the study. Wipe-test and pad-test desorption was carried out using 50 mL syringes, a simple technique that saves time without adversely affecting analyte recovery. Results showed a widespread environmental pollution due to glucocorticoids. This is of particular concern. Evaluation of the dose absorbed by each worker and identification of a biomarker for occupational exposure will contribute to assessment and prevention of occupational exposure.http://dx.doi.org/10.1155/2015/678579 |
| spellingShingle | Enrico Oddone Sara Negri Massimo Bellinzona Silvia Martino Marcello Di Tuccio Elena Grignani Danilo Cottica Marcello Imbriani Determination of Glucocorticoids in UPLC-MS in Environmental Samples from an Occupational Setting International Journal of Analytical Chemistry |
| title | Determination of Glucocorticoids in UPLC-MS in Environmental Samples from an Occupational Setting |
| title_full | Determination of Glucocorticoids in UPLC-MS in Environmental Samples from an Occupational Setting |
| title_fullStr | Determination of Glucocorticoids in UPLC-MS in Environmental Samples from an Occupational Setting |
| title_full_unstemmed | Determination of Glucocorticoids in UPLC-MS in Environmental Samples from an Occupational Setting |
| title_short | Determination of Glucocorticoids in UPLC-MS in Environmental Samples from an Occupational Setting |
| title_sort | determination of glucocorticoids in uplc ms in environmental samples from an occupational setting |
| url | http://dx.doi.org/10.1155/2015/678579 |
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