Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge
Background: Aquaculture has become the fastest-growing sector in recent decades, and this has led to intensified cultural practices to achieve high yields. However, such practices have raised concerns regarding their environmental impact, as aquaculture sludge and wastewater cause significant organi...
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| Language: | English |
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Elsevier
2025-09-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345825000247 |
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| author | Chutema Thongsongkaew Benjamas Cheirsilp Asma Billateh Wageeporn Maneechote Sirasit Srinuanpan |
| author_facet | Chutema Thongsongkaew Benjamas Cheirsilp Asma Billateh Wageeporn Maneechote Sirasit Srinuanpan |
| author_sort | Chutema Thongsongkaew |
| collection | DOAJ |
| description | Background: Aquaculture has become the fastest-growing sector in recent decades, and this has led to intensified cultural practices to achieve high yields. However, such practices have raised concerns regarding their environmental impact, as aquaculture sludge and wastewater cause significant organic pollution. This study then aimed to isolate and screen proteolytic, amylolytic, and lipolytic bacteria and evaluate their consortia for enzyme production and bioremediation of shrimp pond sludge. Results: The strategy using multiple substrates in the isolation media successfully obtained bacterial strains with multiple hydrolytic activities. After primary and secondary quantitative screening, 18 isolates that exhibited high dual and triple hydrolytic activities were selected. After tertiary quantitative screening using synthetic shrimp pond sludge and co-culture tests, Exiguobacterium indicum SSP-PA-08, Bacillus coagulans, and Bacillus subtilis were selected due to their synergy for the production of triple hydrolytic enzymes. Their consortia inoculated in shrimp pond sludge containing 0.8% total suspended solids (TSSs) showed an increase in proteolytic activity by 2.5 folds and amylolytic activity by 20 folds, which led to a greater reduction in TSS. The highest enzyme production was obtained using shrimp pond sludge containing 1.6% TSS. Conclusions: This study has developed the methods to isolate bacteria with multienzyme-producing ability. Co-culturing these bacteria in synthetic shrimp sludge significantly enhanced hydrolytic activity and led to a greater reduction in TSS. These strategies may contribute greatly to the hydrolytic enzyme production and environmentally friendly bioremediation of aquaculture sludge.How to cite: Thongsongkaew C, Cheirsilp B, Billateh A, et al. Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge. Electron J Biotechnol 2025;77. https://doi.org/10.1016/j.ejbt.2025.05.005. |
| format | Article |
| id | doaj-art-01f42fe031634513b14487fc998f2da7 |
| institution | Kabale University |
| issn | 0717-3458 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj-art-01f42fe031634513b14487fc998f2da72025-08-23T04:47:35ZengElsevierElectronic Journal of Biotechnology0717-34582025-09-0177354710.1016/j.ejbt.2025.05.005Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludgeChutema Thongsongkaew0Benjamas Cheirsilp1Asma Billateh2Wageeporn Maneechote3Sirasit Srinuanpan4International Program of Biotechnology, Center of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Program of Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, ThailandInternational Program of Biotechnology, Center of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Program of Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, Thailand; Corresponding author.International Program of Biotechnology, Center of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Program of Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, ThailandCenter of Excellence of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Muang Chiang Mai, Chiang Mai, ThailandCenter of Excellence of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Muang Chiang Mai, Chiang Mai, ThailandBackground: Aquaculture has become the fastest-growing sector in recent decades, and this has led to intensified cultural practices to achieve high yields. However, such practices have raised concerns regarding their environmental impact, as aquaculture sludge and wastewater cause significant organic pollution. This study then aimed to isolate and screen proteolytic, amylolytic, and lipolytic bacteria and evaluate their consortia for enzyme production and bioremediation of shrimp pond sludge. Results: The strategy using multiple substrates in the isolation media successfully obtained bacterial strains with multiple hydrolytic activities. After primary and secondary quantitative screening, 18 isolates that exhibited high dual and triple hydrolytic activities were selected. After tertiary quantitative screening using synthetic shrimp pond sludge and co-culture tests, Exiguobacterium indicum SSP-PA-08, Bacillus coagulans, and Bacillus subtilis were selected due to their synergy for the production of triple hydrolytic enzymes. Their consortia inoculated in shrimp pond sludge containing 0.8% total suspended solids (TSSs) showed an increase in proteolytic activity by 2.5 folds and amylolytic activity by 20 folds, which led to a greater reduction in TSS. The highest enzyme production was obtained using shrimp pond sludge containing 1.6% TSS. Conclusions: This study has developed the methods to isolate bacteria with multienzyme-producing ability. Co-culturing these bacteria in synthetic shrimp sludge significantly enhanced hydrolytic activity and led to a greater reduction in TSS. These strategies may contribute greatly to the hydrolytic enzyme production and environmentally friendly bioremediation of aquaculture sludge.How to cite: Thongsongkaew C, Cheirsilp B, Billateh A, et al. Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge. Electron J Biotechnol 2025;77. https://doi.org/10.1016/j.ejbt.2025.05.005.http://www.sciencedirect.com/science/article/pii/S0717345825000247Amylolytic bacteriaBioremediationCo-cultureConsortiaHydrolytic enzymesLipolytic bacteria |
| spellingShingle | Chutema Thongsongkaew Benjamas Cheirsilp Asma Billateh Wageeporn Maneechote Sirasit Srinuanpan Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge Electronic Journal of Biotechnology Amylolytic bacteria Bioremediation Co-culture Consortia Hydrolytic enzymes Lipolytic bacteria |
| title | Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge |
| title_full | Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge |
| title_fullStr | Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge |
| title_full_unstemmed | Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge |
| title_short | Screening of proteolytic, amylolytic, and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge |
| title_sort | screening of proteolytic amylolytic and lipolytic bacteria and their consortia for concomitant production of hydrolytic enzymes and bioremediation of shrimp pond sludge |
| topic | Amylolytic bacteria Bioremediation Co-culture Consortia Hydrolytic enzymes Lipolytic bacteria |
| url | http://www.sciencedirect.com/science/article/pii/S0717345825000247 |
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