Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR.
Meat products often consist of meat from multiple animal species, and inaccurate food product adulteration and mislabeling can negatively affect consumers. Therefore, a cost-effective and reliable method for identification and quantification of animal species in meat products is required. In this st...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0181949&type=printable |
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| author | Yicun Cai Yuping He Rong Lv Hongchao Chen Qiang Wang Liangwen Pan |
| author_facet | Yicun Cai Yuping He Rong Lv Hongchao Chen Qiang Wang Liangwen Pan |
| author_sort | Yicun Cai |
| collection | DOAJ |
| description | Meat products often consist of meat from multiple animal species, and inaccurate food product adulteration and mislabeling can negatively affect consumers. Therefore, a cost-effective and reliable method for identification and quantification of animal species in meat products is required. In this study, we developed a duplex droplet digital PCR (dddPCR) detection and quantification system to simultaneously identify and quantify the source of meat in samples containing a mixture of beef (Bos taurus) and pork (Sus scrofa) in a single digital PCR reaction tube. Mixed meat samples of known composition were used to test the accuracy and applicability of this method. The limit of detection (LOD) and the limit of quantification (LOQ) of this detection and quantification system were also identified. We conclude that our dddPCR detection and quantification system is suitable for quality control and routine analyses of meat products. |
| format | Article |
| id | doaj-art-01f2af0cb02b467f9ff0b3a5aef52923 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-01f2af0cb02b467f9ff0b3a5aef529232025-08-20T02:03:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018194910.1371/journal.pone.0181949Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR.Yicun CaiYuping HeRong LvHongchao ChenQiang WangLiangwen PanMeat products often consist of meat from multiple animal species, and inaccurate food product adulteration and mislabeling can negatively affect consumers. Therefore, a cost-effective and reliable method for identification and quantification of animal species in meat products is required. In this study, we developed a duplex droplet digital PCR (dddPCR) detection and quantification system to simultaneously identify and quantify the source of meat in samples containing a mixture of beef (Bos taurus) and pork (Sus scrofa) in a single digital PCR reaction tube. Mixed meat samples of known composition were used to test the accuracy and applicability of this method. The limit of detection (LOD) and the limit of quantification (LOQ) of this detection and quantification system were also identified. We conclude that our dddPCR detection and quantification system is suitable for quality control and routine analyses of meat products.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0181949&type=printable |
| spellingShingle | Yicun Cai Yuping He Rong Lv Hongchao Chen Qiang Wang Liangwen Pan Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR. PLoS ONE |
| title | Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR. |
| title_full | Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR. |
| title_fullStr | Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR. |
| title_full_unstemmed | Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR. |
| title_short | Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR. |
| title_sort | detection and quantification of beef and pork materials in meat products by duplex droplet digital pcr |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0181949&type=printable |
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