A Molecular Marker Within the <i>NLP3-B1</i> Gene Is Associated with Earliness in Spring Wheat (<i>Triticum aestivum</i> L.)

Earliness is a critical agronomic trait that enables plants to avoid adverse weather conditions during the late growing season or at harvest. In wheat, earliness is controlled by at least three distinct mechanisms: vernalization requirement, photoperiod sensitivity, and a mechanism independent of th...

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Main Authors: Mikhail Bazhenov, Lyubov Nazarova, Timofey Mokhov, Olga Pukhova, Anastasiya Chernook, Alina Kocheshkova, Pavel Kroupin, Gennady Karlov, Mikhail Divashuk
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/14/12/2888
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Summary:Earliness is a critical agronomic trait that enables plants to avoid adverse weather conditions during the late growing season or at harvest. In wheat, earliness is controlled by at least three distinct mechanisms: vernalization requirement, photoperiod sensitivity, and a mechanism independent of the first two, so called, earliness per se. In this work we report a locus on chromosome 3B within <i>NLP3-B1</i> (TraesCS3B02G190300) gene, coding a nitrate-sensitive transcription factor protein, which is associated with earliness in F<sub>5:6</sub> of PI-518620 x CI-17241 spring wheat cross. The variant ‘A’ of the single nucleotide polymorphism <i>NLP3-B1</i>: c.1824+137G>A, which is proper to evolutionary earlier haplotypes, provides heading and anthesis that is 2 days earlier. The effect of this novel marker on earliness was additive to the effect of <i>PPD-D1</i> locus in the same population; however, the effect of the former was weaker. Similarly, allele <i>Ppd-D1a</i> and the variant ‘A’ of the <i>NLP3-B1</i>: c.1824+137G>A polymorphism statistically significantly reduced the plant height (for 2.2 and 1.3 cm, correspondingly) and changed some other agronomical traits; however, these light pleiotropic effects are not of practical value. The possible direct impact of <i>NLP3-B1</i> on the timing of flowering via altered nitrate sensitivity has been discussed, and other candidate genes on chromosome 3B have also been suggested.
ISSN:2073-4395