Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines.
The purity of chlorophylls plays one of the key role for the production of chlorophyllides. We have designed a facile method for chlorophyll purification by twice solvent extraction. Twice extraction causes the loss of chlorophylls, but the purity of total chlorophylls can be enhanced 182%. Then, th...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0250565&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850042406398853120 |
|---|---|
| author | Yi-Ping Hsiang Yi-Ting Wang Keng-Shiang Huang Ting-Yu Huang Mi-Hsueh Tai Yu-Mei Lin Chih-Hui Yang Jei-Fu Shaw |
| author_facet | Yi-Ping Hsiang Yi-Ting Wang Keng-Shiang Huang Ting-Yu Huang Mi-Hsueh Tai Yu-Mei Lin Chih-Hui Yang Jei-Fu Shaw |
| author_sort | Yi-Ping Hsiang |
| collection | DOAJ |
| description | The purity of chlorophylls plays one of the key role for the production of chlorophyllides. We have designed a facile method for chlorophyll purification by twice solvent extraction. Twice extraction causes the loss of chlorophylls, but the purity of total chlorophylls can be enhanced 182%. Then, the purified chlorophylls can be converted to relatively pure chlorophyllides facilely. The results show that higher purity of chlorophyllides could be obtained when purified chlorophylls (ethanol-hexane extract) was used as starting materials than that of crude chlorophylls (ethanol-only extract). In biocompatibility test, the results showed that the prepared chlorophyllides can be applied as biomaterials. When the prepared chlorophyllides were applied to anticancer tests, they were active both in MCF7 and MDA-MB-231 (multidrug resistant breast cancer cells) cell lines. In addition, the results suggested that the prepared chlorophyllides could be a potential candidate of combination therapy with doxorubicin to breast cancers. |
| format | Article |
| id | doaj-art-01c8da5e8464464daee181a9ec639f32 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-01c8da5e8464464daee181a9ec639f322025-08-20T02:55:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01164e025056510.1371/journal.pone.0250565Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines.Yi-Ping HsiangYi-Ting WangKeng-Shiang HuangTing-Yu HuangMi-Hsueh TaiYu-Mei LinChih-Hui YangJei-Fu ShawThe purity of chlorophylls plays one of the key role for the production of chlorophyllides. We have designed a facile method for chlorophyll purification by twice solvent extraction. Twice extraction causes the loss of chlorophylls, but the purity of total chlorophylls can be enhanced 182%. Then, the purified chlorophylls can be converted to relatively pure chlorophyllides facilely. The results show that higher purity of chlorophyllides could be obtained when purified chlorophylls (ethanol-hexane extract) was used as starting materials than that of crude chlorophylls (ethanol-only extract). In biocompatibility test, the results showed that the prepared chlorophyllides can be applied as biomaterials. When the prepared chlorophyllides were applied to anticancer tests, they were active both in MCF7 and MDA-MB-231 (multidrug resistant breast cancer cells) cell lines. In addition, the results suggested that the prepared chlorophyllides could be a potential candidate of combination therapy with doxorubicin to breast cancers.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0250565&type=printable |
| spellingShingle | Yi-Ping Hsiang Yi-Ting Wang Keng-Shiang Huang Ting-Yu Huang Mi-Hsueh Tai Yu-Mei Lin Chih-Hui Yang Jei-Fu Shaw Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines. PLoS ONE |
| title | Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines. |
| title_full | Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines. |
| title_fullStr | Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines. |
| title_full_unstemmed | Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines. |
| title_short | Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines. |
| title_sort | facile production of chlorophyllides using recombinant crclh1 and their cytotoxicity towards multidrug resistant breast cancer cell lines |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0250565&type=printable |
| work_keys_str_mv | AT yipinghsiang facileproductionofchlorophyllidesusingrecombinantcrclh1andtheircytotoxicitytowardsmultidrugresistantbreastcancercelllines AT yitingwang facileproductionofchlorophyllidesusingrecombinantcrclh1andtheircytotoxicitytowardsmultidrugresistantbreastcancercelllines AT kengshianghuang facileproductionofchlorophyllidesusingrecombinantcrclh1andtheircytotoxicitytowardsmultidrugresistantbreastcancercelllines AT tingyuhuang facileproductionofchlorophyllidesusingrecombinantcrclh1andtheircytotoxicitytowardsmultidrugresistantbreastcancercelllines AT mihsuehtai facileproductionofchlorophyllidesusingrecombinantcrclh1andtheircytotoxicitytowardsmultidrugresistantbreastcancercelllines AT yumeilin facileproductionofchlorophyllidesusingrecombinantcrclh1andtheircytotoxicitytowardsmultidrugresistantbreastcancercelllines AT chihhuiyang facileproductionofchlorophyllidesusingrecombinantcrclh1andtheircytotoxicitytowardsmultidrugresistantbreastcancercelllines AT jeifushaw facileproductionofchlorophyllidesusingrecombinantcrclh1andtheircytotoxicitytowardsmultidrugresistantbreastcancercelllines |