Application of Genomic In Situ Hybridization in Horticultural Science
Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualizati...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/7561909 |
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| author | Fahad Ramzan Adnan Younis Ki-Byung Lim |
| author_facet | Fahad Ramzan Adnan Younis Ki-Byung Lim |
| author_sort | Fahad Ramzan |
| collection | DOAJ |
| description | Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploids of various horticultural crops. Using GISH advancement as multicolor detection is a significant approach to analyze the small and numerous chromosomes in fruit species, for example, Diospyros hybrids. This analytical technique has proved to be the most exact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybrids such as Clivia, Rhododendron, and Lycoris ornamental hybrids. The genome characterization facilitates in hybrid selection having potential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressed sequence chromosomes. This review study epitomizes applications and advancements of genomic in situ hybridization (GISH) techniques in horticultural plants. |
| format | Article |
| id | doaj-art-01a349b3c4d147c8aa35f4e5b13115d7 |
| institution | DOAJ |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-01a349b3c4d147c8aa35f4e5b13115d72025-08-20T03:22:30ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/75619097561909Application of Genomic In Situ Hybridization in Horticultural ScienceFahad Ramzan0Adnan Younis1Ki-Byung Lim2Department of Horticulture, Kyungpook National University, Daegu 41566, Republic of KoreaInstitute of Horticultural Sciences, University of Agriculture, Faisalabad 38040, PakistanDepartment of Horticulture, Kyungpook National University, Daegu 41566, Republic of KoreaMolecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploids of various horticultural crops. Using GISH advancement as multicolor detection is a significant approach to analyze the small and numerous chromosomes in fruit species, for example, Diospyros hybrids. This analytical technique has proved to be the most exact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybrids such as Clivia, Rhododendron, and Lycoris ornamental hybrids. The genome characterization facilitates in hybrid selection having potential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressed sequence chromosomes. This review study epitomizes applications and advancements of genomic in situ hybridization (GISH) techniques in horticultural plants.http://dx.doi.org/10.1155/2017/7561909 |
| spellingShingle | Fahad Ramzan Adnan Younis Ki-Byung Lim Application of Genomic In Situ Hybridization in Horticultural Science International Journal of Genomics |
| title | Application of Genomic In Situ Hybridization in Horticultural Science |
| title_full | Application of Genomic In Situ Hybridization in Horticultural Science |
| title_fullStr | Application of Genomic In Situ Hybridization in Horticultural Science |
| title_full_unstemmed | Application of Genomic In Situ Hybridization in Horticultural Science |
| title_short | Application of Genomic In Situ Hybridization in Horticultural Science |
| title_sort | application of genomic in situ hybridization in horticultural science |
| url | http://dx.doi.org/10.1155/2017/7561909 |
| work_keys_str_mv | AT fahadramzan applicationofgenomicinsituhybridizationinhorticulturalscience AT adnanyounis applicationofgenomicinsituhybridizationinhorticulturalscience AT kibyunglim applicationofgenomicinsituhybridizationinhorticulturalscience |