Identification of Grape <i>NRT</i> Gene Family and Analysis of Its Expression in Leaves Under Nitrogen-Deficiency Stress
Nitrogen, an indispensable macronutrient, significantly affects the appearance, quality, and yield of grapes (<i>Vitis vinifera</i>). Adequate nitrate uptake and intracellular transport, facilitated primarily by nitrate transport (NRT) proteins, are crucial for maintaining nutritional ba...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/11/3/252 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850090619016314880 |
|---|---|
| author | Zhongyi Yang Junjie Mei Wei Zheng Falak Sher Khan Mohammad Nasim Bhuiyan Kangjie Wang Mohammad Saidur Rhaman Naomi Abe-Kanoh Wei Ji |
| author_facet | Zhongyi Yang Junjie Mei Wei Zheng Falak Sher Khan Mohammad Nasim Bhuiyan Kangjie Wang Mohammad Saidur Rhaman Naomi Abe-Kanoh Wei Ji |
| author_sort | Zhongyi Yang |
| collection | DOAJ |
| description | Nitrogen, an indispensable macronutrient, significantly affects the appearance, quality, and yield of grapes (<i>Vitis vinifera</i>). Adequate nitrate uptake and intracellular transport, facilitated primarily by nitrate transport (NRT) proteins, are crucial for maintaining nutritional balance. However, there are no reports on the <i>NRT</i> gene family in grapes. In this study, we identified 53 <i>Nitrate Transporter 1/Peptide Transporter Family</i> (<i>NPF</i>), 3 <i>nitrate transporter 2 family</i> (<i>NRT2</i>), and 1 <i>Nitrate Assimilation-Related 2</i> (<i>NAR2</i>) genes in the grapevine Pinot Noir PN40024 genome. A comprehensive analysis of these gene families, including their physicochemical properties, structural organization, chromosomal distribution, collinearity, cis-acting element distribution, and phylogenetic relationships, revealed the rich diversity and evolutionary conservation of the grapevine <i>Nitrate Transporter</i> (<i>NRT</i>) <i>genes</i>. Furthermore, the expression profiles of <i>VvNRTs</i> in different tissues demonstrated that the <i>NRT</i> genes possess spatio-temporal expression specificity. The expression patterns of the <i>NRT</i> genes were examined by transcriptome sequencing in grapevines across various tissues under nitrogen-deficiency conditions. The expression patterns in grapevine leaves under nitrogen-deficiency conditions showed upregulation of the <i>VvNPF2.3</i> gene in conditions of nitrogen deficiency. This upregulation was strongly associated with a 62.2% reduction in indole-3-acetic acid (IAA) levels and a 21.3% increase in hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) levels, suggesting a complex regulatory response to nitrogen-induced stress. These findings emphasize the potential involvement of <i>NRT</i> genes in the adaptive reaction to nitrogen deficiency and set the stage for future investigations into the molecular mechanisms of nitrogen transportation in grapevines. |
| format | Article |
| id | doaj-art-01a0f228cc6e49fca10e57f8f8806201 |
| institution | DOAJ |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-01a0f228cc6e49fca10e57f8f88062012025-08-20T02:42:32ZengMDPI AGHorticulturae2311-75242025-02-0111325210.3390/horticulturae11030252Identification of Grape <i>NRT</i> Gene Family and Analysis of Its Expression in Leaves Under Nitrogen-Deficiency StressZhongyi Yang0Junjie Mei1Wei Zheng2Falak Sher Khan3Mohammad Nasim Bhuiyan4Kangjie Wang5Mohammad Saidur Rhaman6Naomi Abe-Kanoh7Wei Ji8College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaState Key Laboratory of Wheat Improvement, College of Life Sciences Shandong Agricultural University, Taian 271018, ChinaState Key Laboratory of Wheat Improvement, College of Life Sciences Shandong Agricultural University, Taian 271018, ChinaShanxi Yuncheng Agricultural Vocational and Technical College, Yunchen 044000, ChinaDepartment of Seed Science and Technology, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Food, Life and Environmental Science, Faculty of Agriculture, Yamagata University, Tsuruoka 997-8555, JapanCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaNitrogen, an indispensable macronutrient, significantly affects the appearance, quality, and yield of grapes (<i>Vitis vinifera</i>). Adequate nitrate uptake and intracellular transport, facilitated primarily by nitrate transport (NRT) proteins, are crucial for maintaining nutritional balance. However, there are no reports on the <i>NRT</i> gene family in grapes. In this study, we identified 53 <i>Nitrate Transporter 1/Peptide Transporter Family</i> (<i>NPF</i>), 3 <i>nitrate transporter 2 family</i> (<i>NRT2</i>), and 1 <i>Nitrate Assimilation-Related 2</i> (<i>NAR2</i>) genes in the grapevine Pinot Noir PN40024 genome. A comprehensive analysis of these gene families, including their physicochemical properties, structural organization, chromosomal distribution, collinearity, cis-acting element distribution, and phylogenetic relationships, revealed the rich diversity and evolutionary conservation of the grapevine <i>Nitrate Transporter</i> (<i>NRT</i>) <i>genes</i>. Furthermore, the expression profiles of <i>VvNRTs</i> in different tissues demonstrated that the <i>NRT</i> genes possess spatio-temporal expression specificity. The expression patterns of the <i>NRT</i> genes were examined by transcriptome sequencing in grapevines across various tissues under nitrogen-deficiency conditions. The expression patterns in grapevine leaves under nitrogen-deficiency conditions showed upregulation of the <i>VvNPF2.3</i> gene in conditions of nitrogen deficiency. This upregulation was strongly associated with a 62.2% reduction in indole-3-acetic acid (IAA) levels and a 21.3% increase in hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) levels, suggesting a complex regulatory response to nitrogen-induced stress. These findings emphasize the potential involvement of <i>NRT</i> genes in the adaptive reaction to nitrogen deficiency and set the stage for future investigations into the molecular mechanisms of nitrogen transportation in grapevines.https://www.mdpi.com/2311-7524/11/3/252grapevinesnitrate transporterphylogenetic evolutionexpression analysisabiotic stress |
| spellingShingle | Zhongyi Yang Junjie Mei Wei Zheng Falak Sher Khan Mohammad Nasim Bhuiyan Kangjie Wang Mohammad Saidur Rhaman Naomi Abe-Kanoh Wei Ji Identification of Grape <i>NRT</i> Gene Family and Analysis of Its Expression in Leaves Under Nitrogen-Deficiency Stress Horticulturae grapevines nitrate transporter phylogenetic evolution expression analysis abiotic stress |
| title | Identification of Grape <i>NRT</i> Gene Family and Analysis of Its Expression in Leaves Under Nitrogen-Deficiency Stress |
| title_full | Identification of Grape <i>NRT</i> Gene Family and Analysis of Its Expression in Leaves Under Nitrogen-Deficiency Stress |
| title_fullStr | Identification of Grape <i>NRT</i> Gene Family and Analysis of Its Expression in Leaves Under Nitrogen-Deficiency Stress |
| title_full_unstemmed | Identification of Grape <i>NRT</i> Gene Family and Analysis of Its Expression in Leaves Under Nitrogen-Deficiency Stress |
| title_short | Identification of Grape <i>NRT</i> Gene Family and Analysis of Its Expression in Leaves Under Nitrogen-Deficiency Stress |
| title_sort | identification of grape i nrt i gene family and analysis of its expression in leaves under nitrogen deficiency stress |
| topic | grapevines nitrate transporter phylogenetic evolution expression analysis abiotic stress |
| url | https://www.mdpi.com/2311-7524/11/3/252 |
| work_keys_str_mv | AT zhongyiyang identificationofgrapeinrtigenefamilyandanalysisofitsexpressioninleavesundernitrogendeficiencystress AT junjiemei identificationofgrapeinrtigenefamilyandanalysisofitsexpressioninleavesundernitrogendeficiencystress AT weizheng identificationofgrapeinrtigenefamilyandanalysisofitsexpressioninleavesundernitrogendeficiencystress AT falaksherkhan identificationofgrapeinrtigenefamilyandanalysisofitsexpressioninleavesundernitrogendeficiencystress AT mohammadnasimbhuiyan identificationofgrapeinrtigenefamilyandanalysisofitsexpressioninleavesundernitrogendeficiencystress AT kangjiewang identificationofgrapeinrtigenefamilyandanalysisofitsexpressioninleavesundernitrogendeficiencystress AT mohammadsaidurrhaman identificationofgrapeinrtigenefamilyandanalysisofitsexpressioninleavesundernitrogendeficiencystress AT naomiabekanoh identificationofgrapeinrtigenefamilyandanalysisofitsexpressioninleavesundernitrogendeficiencystress AT weiji identificationofgrapeinrtigenefamilyandanalysisofitsexpressioninleavesundernitrogendeficiencystress |