Enzymatic synthesis of some sugar-lauric acid esters by lipase from Candida antarctica and their functionalities as emulsifiers and antibacterial agents

To investigate the regioselectivity of lipase-catalyzed synthesis of important sugar-laurate esters and their functionalities as emulsifiers and antimicrobial agents, glucose, galactose, mannose, maltose and trehalose were used. 6-O-lauryl glucose (Glu-L), 6-O-lauryl galactose (Gal-L), 6-O-lauryl ma...

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Main Authors: Kangzi Ren, Guilin Chen, Ziyi Zhang, Zhao Long, Bo Zhou, Wenfang Han, Qinlu Lin
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Food Chemistry: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590157525002305
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author Kangzi Ren
Guilin Chen
Ziyi Zhang
Zhao Long
Bo Zhou
Wenfang Han
Qinlu Lin
author_facet Kangzi Ren
Guilin Chen
Ziyi Zhang
Zhao Long
Bo Zhou
Wenfang Han
Qinlu Lin
author_sort Kangzi Ren
collection DOAJ
description To investigate the regioselectivity of lipase-catalyzed synthesis of important sugar-laurate esters and their functionalities as emulsifiers and antimicrobial agents, glucose, galactose, mannose, maltose and trehalose were used. 6-O-lauryl glucose (Glu-L), 6-O-lauryl galactose (Gal-L), 6-O-lauryl mannose (Man-L), 6′-O-lauryl maltose (Mal-L) and 6-O-lauryl trehalose/6’-O-lauryl trehalose (Tre-L) were synthesized using lipase from Candida antarctica. The Glu-L and Man-L achieved the highest yields (65.49 % and 58.16 %, respectively). The synthesized esters produced smaller-particle-size (1.9–3.1 μm), but less stable emulsions than the commercial sucrose esters (4.2–8.1 μm). The zeta-potential data revealed Man-L and Gal-L had higher surface coverage than the Glu-L on the oil droplets, while the Mal-L and Tre-L had similar surface coverage. The Man-L, Mal-L and Tre-L demonstrated the superior foamability and foam stability. The Gal-L, Man-L, Mal-L, and Tre-L inhibited E.coli 12024 (MIC 1–4 mg/mL), while only Man-L, Mal-L, and Tre-L inhibited B.subtilis 5009 (MIC 0.5–2.0 mg/mL).
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publishDate 2025-04-01
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series Food Chemistry: X
spelling doaj-art-019f54f87bad44fcb854f3e0821c337d2025-08-20T02:30:50ZengElsevierFood Chemistry: X2590-15752025-04-012710238310.1016/j.fochx.2025.102383Enzymatic synthesis of some sugar-lauric acid esters by lipase from Candida antarctica and their functionalities as emulsifiers and antibacterial agentsKangzi Ren0Guilin Chen1Ziyi Zhang2Zhao Long3Bo Zhou4Wenfang Han5Qinlu Lin6College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; National Engineering Research Center of Rice and Byproduct Deep Processing, 498 South Shaoshan Road, Hunan 419994, ChinaCollege of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; National Engineering Research Center of Rice and Byproduct Deep Processing, 498 South Shaoshan Road, Hunan 419994, ChinaCollege of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; National Engineering Research Center of Rice and Byproduct Deep Processing, 498 South Shaoshan Road, Hunan 419994, ChinaCollege of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; National Engineering Research Center of Rice and Byproduct Deep Processing, 498 South Shaoshan Road, Hunan 419994, ChinaCollege of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; National Engineering Research Center of Rice and Byproduct Deep Processing, 498 South Shaoshan Road, Hunan 419994, ChinaCollege of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; National Engineering Research Center of Rice and Byproduct Deep Processing, 498 South Shaoshan Road, Hunan 419994, ChinaCollege of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; National Engineering Research Center of Rice and Byproduct Deep Processing, 498 South Shaoshan Road, Hunan 419994, China; Corresponding author at: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.To investigate the regioselectivity of lipase-catalyzed synthesis of important sugar-laurate esters and their functionalities as emulsifiers and antimicrobial agents, glucose, galactose, mannose, maltose and trehalose were used. 6-O-lauryl glucose (Glu-L), 6-O-lauryl galactose (Gal-L), 6-O-lauryl mannose (Man-L), 6′-O-lauryl maltose (Mal-L) and 6-O-lauryl trehalose/6’-O-lauryl trehalose (Tre-L) were synthesized using lipase from Candida antarctica. The Glu-L and Man-L achieved the highest yields (65.49 % and 58.16 %, respectively). The synthesized esters produced smaller-particle-size (1.9–3.1 μm), but less stable emulsions than the commercial sucrose esters (4.2–8.1 μm). The zeta-potential data revealed Man-L and Gal-L had higher surface coverage than the Glu-L on the oil droplets, while the Mal-L and Tre-L had similar surface coverage. The Man-L, Mal-L and Tre-L demonstrated the superior foamability and foam stability. The Gal-L, Man-L, Mal-L, and Tre-L inhibited E.coli 12024 (MIC 1–4 mg/mL), while only Man-L, Mal-L, and Tre-L inhibited B.subtilis 5009 (MIC 0.5–2.0 mg/mL).http://www.sciencedirect.com/science/article/pii/S2590157525002305Sugar esterLauric acidLipase-catalyzed synthesisEmulsifiersFoamAntibacterial
spellingShingle Kangzi Ren
Guilin Chen
Ziyi Zhang
Zhao Long
Bo Zhou
Wenfang Han
Qinlu Lin
Enzymatic synthesis of some sugar-lauric acid esters by lipase from Candida antarctica and their functionalities as emulsifiers and antibacterial agents
Food Chemistry: X
Sugar ester
Lauric acid
Lipase-catalyzed synthesis
Emulsifiers
Foam
Antibacterial
title Enzymatic synthesis of some sugar-lauric acid esters by lipase from Candida antarctica and their functionalities as emulsifiers and antibacterial agents
title_full Enzymatic synthesis of some sugar-lauric acid esters by lipase from Candida antarctica and their functionalities as emulsifiers and antibacterial agents
title_fullStr Enzymatic synthesis of some sugar-lauric acid esters by lipase from Candida antarctica and their functionalities as emulsifiers and antibacterial agents
title_full_unstemmed Enzymatic synthesis of some sugar-lauric acid esters by lipase from Candida antarctica and their functionalities as emulsifiers and antibacterial agents
title_short Enzymatic synthesis of some sugar-lauric acid esters by lipase from Candida antarctica and their functionalities as emulsifiers and antibacterial agents
title_sort enzymatic synthesis of some sugar lauric acid esters by lipase from candida antarctica and their functionalities as emulsifiers and antibacterial agents
topic Sugar ester
Lauric acid
Lipase-catalyzed synthesis
Emulsifiers
Foam
Antibacterial
url http://www.sciencedirect.com/science/article/pii/S2590157525002305
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