Transcriptome analysis of Ochratoxin a (OTA) producing Aspergillus westerdijkiae fc-1 under varying osmotic pressure
Ochratoxin A (OTA) is a toxic secondary metabolite produced by the Aspergillus species which can contaminate various food products. This study analysed the transcriptome of the Aspergillus westerdijkiae fc-1 strain under NaCl concentrations of 0, 20, and 100 g/L using RNA-Seq technology to examine g...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-04-01
|
| Series: | Mycology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21501203.2024.2408259 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850153547621990400 |
|---|---|
| author | Yanling Ma Muyuan Zhuang Tanvir Ahmad Mingxuan Li Guangyou Tan Yingyao Deng Yang Liu |
| author_facet | Yanling Ma Muyuan Zhuang Tanvir Ahmad Mingxuan Li Guangyou Tan Yingyao Deng Yang Liu |
| author_sort | Yanling Ma |
| collection | DOAJ |
| description | Ochratoxin A (OTA) is a toxic secondary metabolite produced by the Aspergillus species which can contaminate various food products. This study analysed the transcriptome of the Aspergillus westerdijkiae fc-1 strain under NaCl concentrations of 0, 20, and 100 g/L using RNA-Seq technology to examine gene transcriptional changes linked to osmotic stress and OTA production. Significant changes were observed in metabolic-pathways associated with carbohydrates, cellular communication, and hydrolase activity under 20 g/L NaCl. The HOG1 gene, associated with osmotic pressure regulation was down-regulated by 78.06%. In contrast, OTA biosynthesis genes otaA, otaB, and otaC were up-regulated by 3.26 fold, 1.99 fold, and 2.06 fold, respectively. Conversely, the otaD gene was down-regulated by 43.50%. At 100 g/L NaCl, pathways related to ion transport, peptide metabolism, ribosomal function, and transmembrane transporter protein activities were significantly up-regulated. The HOG1 gene was up-regulated by 28.32% and OTA biosynthesis genes otaA, otaB, otaC, and otaD showed up-regulation of 27.06%, 36.80%, 19.59%, and 5.72 fold, respectively. The study highlights the role of metabolic pathways in osmotic stress regulation and the correlations between HOG1 expression and OTA biosynthesis genes, providing insights for developing strategies to prevent OTA contamination in food. |
| format | Article |
| id | doaj-art-0192abf8fdbf4e459f579798ec79810a |
| institution | OA Journals |
| issn | 2150-1203 2150-1211 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycology |
| spelling | doaj-art-0192abf8fdbf4e459f579798ec79810a2025-08-20T02:25:41ZengTaylor & Francis GroupMycology2150-12032150-12112025-04-0116290391710.1080/21501203.2024.2408259Transcriptome analysis of Ochratoxin a (OTA) producing Aspergillus westerdijkiae fc-1 under varying osmotic pressureYanling Ma0Muyuan Zhuang1Tanvir Ahmad2Mingxuan Li3Guangyou Tan4Yingyao Deng5Yang Liu6School of Food Science and Engineering, Foshan University/National Technical Center (Foshan) for Quality Control of Famous and Special Agricultural Products/Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan, ChinaSchool of Food Science and Engineering, Foshan University/National Technical Center (Foshan) for Quality Control of Famous and Special Agricultural Products/Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan, ChinaSchool of Food Science and Engineering, Foshan University/National Technical Center (Foshan) for Quality Control of Famous and Special Agricultural Products/Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan, ChinaSchool of Food Science and Engineering, Foshan University/National Technical Center (Foshan) for Quality Control of Famous and Special Agricultural Products/Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan, ChinaSchool of Food Science and Engineering, Foshan University/National Technical Center (Foshan) for Quality Control of Famous and Special Agricultural Products/Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan, ChinaSchool of Food Science and Engineering, Foshan University/National Technical Center (Foshan) for Quality Control of Famous and Special Agricultural Products/Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan, ChinaSchool of Food Science and Engineering, Foshan University/National Technical Center (Foshan) for Quality Control of Famous and Special Agricultural Products/Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan, ChinaOchratoxin A (OTA) is a toxic secondary metabolite produced by the Aspergillus species which can contaminate various food products. This study analysed the transcriptome of the Aspergillus westerdijkiae fc-1 strain under NaCl concentrations of 0, 20, and 100 g/L using RNA-Seq technology to examine gene transcriptional changes linked to osmotic stress and OTA production. Significant changes were observed in metabolic-pathways associated with carbohydrates, cellular communication, and hydrolase activity under 20 g/L NaCl. The HOG1 gene, associated with osmotic pressure regulation was down-regulated by 78.06%. In contrast, OTA biosynthesis genes otaA, otaB, and otaC were up-regulated by 3.26 fold, 1.99 fold, and 2.06 fold, respectively. Conversely, the otaD gene was down-regulated by 43.50%. At 100 g/L NaCl, pathways related to ion transport, peptide metabolism, ribosomal function, and transmembrane transporter protein activities were significantly up-regulated. The HOG1 gene was up-regulated by 28.32% and OTA biosynthesis genes otaA, otaB, otaC, and otaD showed up-regulation of 27.06%, 36.80%, 19.59%, and 5.72 fold, respectively. The study highlights the role of metabolic pathways in osmotic stress regulation and the correlations between HOG1 expression and OTA biosynthesis genes, providing insights for developing strategies to prevent OTA contamination in food.https://www.tandfonline.com/doi/10.1080/21501203.2024.2408259OTAAspergillus westerdijkiaeNaClRNA-SeqHOG signalling cascade |
| spellingShingle | Yanling Ma Muyuan Zhuang Tanvir Ahmad Mingxuan Li Guangyou Tan Yingyao Deng Yang Liu Transcriptome analysis of Ochratoxin a (OTA) producing Aspergillus westerdijkiae fc-1 under varying osmotic pressure Mycology OTA Aspergillus westerdijkiae NaCl RNA-Seq HOG signalling cascade |
| title | Transcriptome analysis of Ochratoxin a (OTA) producing Aspergillus westerdijkiae fc-1 under varying osmotic pressure |
| title_full | Transcriptome analysis of Ochratoxin a (OTA) producing Aspergillus westerdijkiae fc-1 under varying osmotic pressure |
| title_fullStr | Transcriptome analysis of Ochratoxin a (OTA) producing Aspergillus westerdijkiae fc-1 under varying osmotic pressure |
| title_full_unstemmed | Transcriptome analysis of Ochratoxin a (OTA) producing Aspergillus westerdijkiae fc-1 under varying osmotic pressure |
| title_short | Transcriptome analysis of Ochratoxin a (OTA) producing Aspergillus westerdijkiae fc-1 under varying osmotic pressure |
| title_sort | transcriptome analysis of ochratoxin a ota producing aspergillus westerdijkiae fc 1 under varying osmotic pressure |
| topic | OTA Aspergillus westerdijkiae NaCl RNA-Seq HOG signalling cascade |
| url | https://www.tandfonline.com/doi/10.1080/21501203.2024.2408259 |
| work_keys_str_mv | AT yanlingma transcriptomeanalysisofochratoxinaotaproducingaspergilluswesterdijkiaefc1undervaryingosmoticpressure AT muyuanzhuang transcriptomeanalysisofochratoxinaotaproducingaspergilluswesterdijkiaefc1undervaryingosmoticpressure AT tanvirahmad transcriptomeanalysisofochratoxinaotaproducingaspergilluswesterdijkiaefc1undervaryingosmoticpressure AT mingxuanli transcriptomeanalysisofochratoxinaotaproducingaspergilluswesterdijkiaefc1undervaryingosmoticpressure AT guangyoutan transcriptomeanalysisofochratoxinaotaproducingaspergilluswesterdijkiaefc1undervaryingosmoticpressure AT yingyaodeng transcriptomeanalysisofochratoxinaotaproducingaspergilluswesterdijkiaefc1undervaryingosmoticpressure AT yangliu transcriptomeanalysisofochratoxinaotaproducingaspergilluswesterdijkiaefc1undervaryingosmoticpressure |