Distinct Expression Patterns of Apoptosis and Autophagy-Associated Proteins and Genes during Postnatal Development of Spiral Ganglion Neurons in Rat
Autophagy and apoptosis have a complex interplay in the early embryo development. The development of spiral ganglion neurons (SGNs) in addition to Corti’s organ in the mammalian cochlea remains crucial in the first two-week postnatal period. To investigate the roles of apoptosis and autophagy in the...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2020/9387560 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560292738891776 |
|---|---|
| author | Shule Hou Penghui Chen Jiarui Chen Junmin Chen Lianhua Sun Jianyong Chen Baihui He Yue Li Huan Qin Yuren Hong Shuna Li Jingchun He Dekun Gao Fabio Mammano Jun Yang |
| author_facet | Shule Hou Penghui Chen Jiarui Chen Junmin Chen Lianhua Sun Jianyong Chen Baihui He Yue Li Huan Qin Yuren Hong Shuna Li Jingchun He Dekun Gao Fabio Mammano Jun Yang |
| author_sort | Shule Hou |
| collection | DOAJ |
| description | Autophagy and apoptosis have a complex interplay in the early embryo development. The development of spiral ganglion neurons (SGNs) in addition to Corti’s organ in the mammalian cochlea remains crucial in the first two-week postnatal period. To investigate the roles of apoptosis and autophagy in the development of SGNs, light microscopy was used to observe the morphological changes of SGNs. The number of SGNs was decreased from P1 to P7 and plateaued from P10 to P14. Immunohistochemistry results revealed positive expression of cleaved-caspase3, bcl-2, microtubule-associated protein light chain 3-II (LC3-II), Beclin1, and sequestosome 1 (SQSTM1/P62) in SGNs. The apoptotic bodies and autophagosomes and autolysosomes were also identified by transmission electron microscopy at P1 and P7. Real-time PCR and western blotting results revealed that the apoptotic activity peaked at P7 and the autophagy activity was gradually upregulated along with the development. Taken together, our results for the first time showed that autophagy and apoptosis in SGNs play distinct roles during specific developmental phases in a time-dependent manner. |
| format | Article |
| id | doaj-art-018c9e951bd145498b7dfdc3a805a3a1 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-018c9e951bd145498b7dfdc3a805a3a12025-02-03T01:27:55ZengWileyNeural Plasticity2090-59041687-54432020-01-01202010.1155/2020/93875609387560Distinct Expression Patterns of Apoptosis and Autophagy-Associated Proteins and Genes during Postnatal Development of Spiral Ganglion Neurons in RatShule Hou0Penghui Chen1Jiarui Chen2Junmin Chen3Lianhua Sun4Jianyong Chen5Baihui He6Yue Li7Huan Qin8Yuren Hong9Shuna Li10Jingchun He11Dekun Gao12Fabio Mammano13Jun Yang14Department of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Shanghai Children’s Hospital, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaLaboratory of Electron Microscope Center, Shanghai Medical College, Fudan University, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Physics and Astronomy “G. Galilei”, University of Padua, Padova, ItalyDepartment of Otorhinolaryngology-Head & Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaAutophagy and apoptosis have a complex interplay in the early embryo development. The development of spiral ganglion neurons (SGNs) in addition to Corti’s organ in the mammalian cochlea remains crucial in the first two-week postnatal period. To investigate the roles of apoptosis and autophagy in the development of SGNs, light microscopy was used to observe the morphological changes of SGNs. The number of SGNs was decreased from P1 to P7 and plateaued from P10 to P14. Immunohistochemistry results revealed positive expression of cleaved-caspase3, bcl-2, microtubule-associated protein light chain 3-II (LC3-II), Beclin1, and sequestosome 1 (SQSTM1/P62) in SGNs. The apoptotic bodies and autophagosomes and autolysosomes were also identified by transmission electron microscopy at P1 and P7. Real-time PCR and western blotting results revealed that the apoptotic activity peaked at P7 and the autophagy activity was gradually upregulated along with the development. Taken together, our results for the first time showed that autophagy and apoptosis in SGNs play distinct roles during specific developmental phases in a time-dependent manner.http://dx.doi.org/10.1155/2020/9387560 |
| spellingShingle | Shule Hou Penghui Chen Jiarui Chen Junmin Chen Lianhua Sun Jianyong Chen Baihui He Yue Li Huan Qin Yuren Hong Shuna Li Jingchun He Dekun Gao Fabio Mammano Jun Yang Distinct Expression Patterns of Apoptosis and Autophagy-Associated Proteins and Genes during Postnatal Development of Spiral Ganglion Neurons in Rat Neural Plasticity |
| title | Distinct Expression Patterns of Apoptosis and Autophagy-Associated Proteins and Genes during Postnatal Development of Spiral Ganglion Neurons in Rat |
| title_full | Distinct Expression Patterns of Apoptosis and Autophagy-Associated Proteins and Genes during Postnatal Development of Spiral Ganglion Neurons in Rat |
| title_fullStr | Distinct Expression Patterns of Apoptosis and Autophagy-Associated Proteins and Genes during Postnatal Development of Spiral Ganglion Neurons in Rat |
| title_full_unstemmed | Distinct Expression Patterns of Apoptosis and Autophagy-Associated Proteins and Genes during Postnatal Development of Spiral Ganglion Neurons in Rat |
| title_short | Distinct Expression Patterns of Apoptosis and Autophagy-Associated Proteins and Genes during Postnatal Development of Spiral Ganglion Neurons in Rat |
| title_sort | distinct expression patterns of apoptosis and autophagy associated proteins and genes during postnatal development of spiral ganglion neurons in rat |
| url | http://dx.doi.org/10.1155/2020/9387560 |
| work_keys_str_mv | AT shulehou distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT penghuichen distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT jiaruichen distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT junminchen distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT lianhuasun distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT jianyongchen distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT baihuihe distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT yueli distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT huanqin distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT yurenhong distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT shunali distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT jingchunhe distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT dekungao distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT fabiomammano distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat AT junyang distinctexpressionpatternsofapoptosisandautophagyassociatedproteinsandgenesduringpostnataldevelopmentofspiralganglionneuronsinrat |