Design of warp-knitted textile carriers for biofilm growth: towards scalable application in MBBR systems

Abstract Textile-based carriers represent a promising alternative to conventional plastic media used in moving-bed biofilm reactors (MBBRs) for biological wastewater treatment. In this study, we developed and evaluated warp-knitted multifilament textile carriers intended to support biofilm growth, p...

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Main Authors: Irena Lenfeldová, Martina Ryvolová, Adéla Kdýrová
Format: Article
Language:English
Published: Springer 2025-08-01
Series:Discover Applied Sciences
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Online Access:https://doi.org/10.1007/s42452-025-07640-8
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author Irena Lenfeldová
Martina Ryvolová
Adéla Kdýrová
author_facet Irena Lenfeldová
Martina Ryvolová
Adéla Kdýrová
author_sort Irena Lenfeldová
collection DOAJ
description Abstract Textile-based carriers represent a promising alternative to conventional plastic media used in moving-bed biofilm reactors (MBBRs) for biological wastewater treatment. In this study, we developed and evaluated warp-knitted multifilament textile carriers intended to support biofilm growth, particularly under post-nitrification conditions. Four carrier variants were fabricated using combinations of polypropylene (PP) and polyester (PES) yarns to adjust material buoyancy (weight was in the range of 0.596 g to 0.612 g), dimension, 3D structures, and high surface availability for biofilm growth. The effective carrier area ranged from 4.6 × 10³ mm² to 5.3 × 103 mm2. Biofilm development was assessed through image analysis and oxygen uptake rate measurements. The positive effect of the holes on the water flowing through the carriers was not proven, but the long multifilament segment and the fibrous surface support bacterial growth. The warp-knitted spacer carriers developed in this study successfully supported the formation of active biofilms, as indicated by elevated oxygen consumption rates (OCR) reaching up to 8 mg O₂/L/h within six weeks of operation (OCR of 4–5 mg O₂/L/h was recorded for the plastic carrier). Only minor differences were observed between measurements taken at weeks 6 and 14, suggesting early stabilization of biofilm activity. Warp-knitted textile carriers show strong potential as biofilm-supporting media due to their structural flexibility, low weight, and scalability. Although compatibility with industrial systems has not yet been confirmed, the carriers were designed with this objective in mind and demonstrated rapid colonization and promising performance at the laboratory scale, suggesting their potential suitability for industrial applications. Graphical Abstract
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spelling doaj-art-01899bd457374b4d92955c5d8a503f9f2025-08-24T11:45:17ZengSpringerDiscover Applied Sciences3004-92612025-08-017911610.1007/s42452-025-07640-8Design of warp-knitted textile carriers for biofilm growth: towards scalable application in MBBR systemsIrena Lenfeldová0Martina Ryvolová1Adéla Kdýrová2Faculty of Textile Engineering Department of Technologies and Structures, Technical University of LiberecFaculty of Textile Engineering Department of Technologies and Structures, Technical University of LiberecFaculty of Textile Engineering Department of Technologies and Structures, Technical University of LiberecAbstract Textile-based carriers represent a promising alternative to conventional plastic media used in moving-bed biofilm reactors (MBBRs) for biological wastewater treatment. In this study, we developed and evaluated warp-knitted multifilament textile carriers intended to support biofilm growth, particularly under post-nitrification conditions. Four carrier variants were fabricated using combinations of polypropylene (PP) and polyester (PES) yarns to adjust material buoyancy (weight was in the range of 0.596 g to 0.612 g), dimension, 3D structures, and high surface availability for biofilm growth. The effective carrier area ranged from 4.6 × 10³ mm² to 5.3 × 103 mm2. Biofilm development was assessed through image analysis and oxygen uptake rate measurements. The positive effect of the holes on the water flowing through the carriers was not proven, but the long multifilament segment and the fibrous surface support bacterial growth. The warp-knitted spacer carriers developed in this study successfully supported the formation of active biofilms, as indicated by elevated oxygen consumption rates (OCR) reaching up to 8 mg O₂/L/h within six weeks of operation (OCR of 4–5 mg O₂/L/h was recorded for the plastic carrier). Only minor differences were observed between measurements taken at weeks 6 and 14, suggesting early stabilization of biofilm activity. Warp-knitted textile carriers show strong potential as biofilm-supporting media due to their structural flexibility, low weight, and scalability. Although compatibility with industrial systems has not yet been confirmed, the carriers were designed with this objective in mind and demonstrated rapid colonization and promising performance at the laboratory scale, suggesting their potential suitability for industrial applications. Graphical Abstracthttps://doi.org/10.1007/s42452-025-07640-8Textile biomass carrierBiofilm captureWarp knitting technologyImage analysisWastewater treatment
spellingShingle Irena Lenfeldová
Martina Ryvolová
Adéla Kdýrová
Design of warp-knitted textile carriers for biofilm growth: towards scalable application in MBBR systems
Discover Applied Sciences
Textile biomass carrier
Biofilm capture
Warp knitting technology
Image analysis
Wastewater treatment
title Design of warp-knitted textile carriers for biofilm growth: towards scalable application in MBBR systems
title_full Design of warp-knitted textile carriers for biofilm growth: towards scalable application in MBBR systems
title_fullStr Design of warp-knitted textile carriers for biofilm growth: towards scalable application in MBBR systems
title_full_unstemmed Design of warp-knitted textile carriers for biofilm growth: towards scalable application in MBBR systems
title_short Design of warp-knitted textile carriers for biofilm growth: towards scalable application in MBBR systems
title_sort design of warp knitted textile carriers for biofilm growth towards scalable application in mbbr systems
topic Textile biomass carrier
Biofilm capture
Warp knitting technology
Image analysis
Wastewater treatment
url https://doi.org/10.1007/s42452-025-07640-8
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