Single-cell RNA sequencing in tuberculosis: Application and future perspectives
Abstract. Tuberculosis (TB) has one of the highest mortality rates among infectious diseases worldwide. The immune response in the host after infection is proposed to contribute significantly to the progression of TB, but the specific mechanisms involved remain to be elucidated. Single-cell RNA sequ...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2025-07-01
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| Series: | Chinese Medical Journal |
| Online Access: | http://journals.lww.com/10.1097/CM9.0000000000003095 |
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| author | Yuejuan Zhan Qiran Zhang Wenyang Wang Wenyi Liang Chengdi Wang Peifang Wei |
| author_facet | Yuejuan Zhan Qiran Zhang Wenyang Wang Wenyi Liang Chengdi Wang Peifang Wei |
| author_sort | Yuejuan Zhan |
| collection | DOAJ |
| description | Abstract. Tuberculosis (TB) has one of the highest mortality rates among infectious diseases worldwide. The immune response in the host after infection is proposed to contribute significantly to the progression of TB, but the specific mechanisms involved remain to be elucidated. Single-cell RNA sequencing (scRNA-seq) provides unbiased transcriptome sequencing of large quantities of individual cells, thereby defining biological comprehension of cellular heterogeneity and dynamic transcriptome state of cell populations in the field of immunology and is therefore increasingly applied to lung disease research. Here, we first briefly introduce the concept of scRNA-seq, followed by a summarization on the application of scRNA-seq to TB. Furthermore, we underscore the potential of scRNA-seq for clinical biomarker exploration, host-directed therapy, and precision therapy research in TB and discuss the bottlenecks that need to be overcome for the broad application of scRNA-seq to TB-related research. |
| format | Article |
| id | doaj-art-018098e1312d4aa7bd2af48e5f0262ac |
| institution | Kabale University |
| issn | 0366-6999 2542-5641 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj-art-018098e1312d4aa7bd2af48e5f0262ac2025-08-20T03:50:59ZengWolters KluwerChinese Medical Journal0366-69992542-56412025-07-01138141676168610.1097/CM9.0000000000003095202507200-00005Single-cell RNA sequencing in tuberculosis: Application and future perspectivesYuejuan Zhan0Qiran Zhang1Wenyang Wang2Wenyi Liang3Chengdi Wang4Peifang Wei1 Department of Pulmonary and Critical Care Medicine, Targeted Tracer Research and Development Laboratory, State Key Laboratory of Respiratory Health and Multimorbidity, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China2 West China School of Medicine, Sichuan University, Chengdu, Sichuan 610041, China3 Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China2 West China School of Medicine, Sichuan University, Chengdu, Sichuan 610041, China1 Department of Pulmonary and Critical Care Medicine, Targeted Tracer Research and Development Laboratory, State Key Laboratory of Respiratory Health and Multimorbidity, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaAbstract. Tuberculosis (TB) has one of the highest mortality rates among infectious diseases worldwide. The immune response in the host after infection is proposed to contribute significantly to the progression of TB, but the specific mechanisms involved remain to be elucidated. Single-cell RNA sequencing (scRNA-seq) provides unbiased transcriptome sequencing of large quantities of individual cells, thereby defining biological comprehension of cellular heterogeneity and dynamic transcriptome state of cell populations in the field of immunology and is therefore increasingly applied to lung disease research. Here, we first briefly introduce the concept of scRNA-seq, followed by a summarization on the application of scRNA-seq to TB. Furthermore, we underscore the potential of scRNA-seq for clinical biomarker exploration, host-directed therapy, and precision therapy research in TB and discuss the bottlenecks that need to be overcome for the broad application of scRNA-seq to TB-related research.http://journals.lww.com/10.1097/CM9.0000000000003095 |
| spellingShingle | Yuejuan Zhan Qiran Zhang Wenyang Wang Wenyi Liang Chengdi Wang Peifang Wei Single-cell RNA sequencing in tuberculosis: Application and future perspectives Chinese Medical Journal |
| title | Single-cell RNA sequencing in tuberculosis: Application and future perspectives |
| title_full | Single-cell RNA sequencing in tuberculosis: Application and future perspectives |
| title_fullStr | Single-cell RNA sequencing in tuberculosis: Application and future perspectives |
| title_full_unstemmed | Single-cell RNA sequencing in tuberculosis: Application and future perspectives |
| title_short | Single-cell RNA sequencing in tuberculosis: Application and future perspectives |
| title_sort | single cell rna sequencing in tuberculosis application and future perspectives |
| url | http://journals.lww.com/10.1097/CM9.0000000000003095 |
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