Evaluation of Peri-Implant Bone Repair in Ovariectomized Rats Submitted to the Implant Placement Functionalized with Anti-Sclerostin
(1) Background: The challenges in Implantology involve the development of alternative methods to enhance bone repair in patients with systemic conditions, such as osteoporosis. This study aimed to evaluate the effect of a local anti-sclerostin monoclonal antibody (Scl-Ab) on the functionalization of...
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2025-03-01
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| author | Pedro Henrique Silva Gomes-Ferreira Paula Buzo Frigério Nathália Dantas Duarte Juliana de Moura Naara Gabriela Monteiro André Luis da Silva Fabris Roberta Okamoto |
| author_facet | Pedro Henrique Silva Gomes-Ferreira Paula Buzo Frigério Nathália Dantas Duarte Juliana de Moura Naara Gabriela Monteiro André Luis da Silva Fabris Roberta Okamoto |
| author_sort | Pedro Henrique Silva Gomes-Ferreira |
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| description | (1) Background: The challenges in Implantology involve the development of alternative methods to enhance bone repair in patients with systemic conditions, such as osteoporosis. This study aimed to evaluate the effect of a local anti-sclerostin monoclonal antibody (Scl-Ab) on the functionalization of titanium implant surfaces through a dip-coating technique in peri-implant bone repair. (2) Methods: A total of 32 female rats were separated into four groups (n = 8): SHAM NT (Sham surgery), OVX NT (ovariectomy), SHAM Scl-Ab (SHAM; implants functionalized with Scl-Ab), and OVX Scl-Ab (OVX; implants functionalized with Scl-Ab). Implant surgery was executed 30 days after ovariectomy, and the rats were euthanized 28 days postoperatively. The right tibia was used for removal torque and RT-PCR, while the left tibia was collected for micro-CT and laser confocal microscopy. (3) Results: Functionalization with Scl-Ab significantly increased the gene expression of bone markers, especially ALP, in the SHAM Scl-Ab group compared to the other groups (<i>p</i> < 0.05). (4) Conclusions: Some parameters of this study indicate that implants functionalized with anti-sclerostin bone anabolic drug enhance peri-implant bone repair, especially in healthy rats. However, more studies must be carried out to confirm the therapeutic benefits of this approach. |
| format | Article |
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| language | English |
| publishDate | 2025-03-01 |
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| series | Bioengineering |
| spelling | doaj-art-01757e6d4bb44c95a56fcd149c5119d32025-08-20T02:28:18ZengMDPI AGBioengineering2306-53542025-03-0112435810.3390/bioengineering12040358Evaluation of Peri-Implant Bone Repair in Ovariectomized Rats Submitted to the Implant Placement Functionalized with Anti-SclerostinPedro Henrique Silva Gomes-Ferreira0Paula Buzo Frigério1Nathália Dantas Duarte2Juliana de Moura3Naara Gabriela Monteiro4André Luis da Silva Fabris5Roberta Okamoto6Department of Dentistry, Ourinhos University Center (UNIFIO), Ourinhos 19909-100, SP, BrazilDepartment of Diagnosis and Surgery, Araçatuba School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, BrazilDepartment of Diagnosis and Surgery, Araçatuba School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, BrazilDepartment of Diagnosis and Surgery, Araçatuba School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, BrazilDepartment of Diagnosis and Surgery, Araçatuba School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, BrazilDepartment of Diagnosis and Surgery, Araçatuba School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, BrazilDepartment of Basic Sciences, Araçatuba School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil(1) Background: The challenges in Implantology involve the development of alternative methods to enhance bone repair in patients with systemic conditions, such as osteoporosis. This study aimed to evaluate the effect of a local anti-sclerostin monoclonal antibody (Scl-Ab) on the functionalization of titanium implant surfaces through a dip-coating technique in peri-implant bone repair. (2) Methods: A total of 32 female rats were separated into four groups (n = 8): SHAM NT (Sham surgery), OVX NT (ovariectomy), SHAM Scl-Ab (SHAM; implants functionalized with Scl-Ab), and OVX Scl-Ab (OVX; implants functionalized with Scl-Ab). Implant surgery was executed 30 days after ovariectomy, and the rats were euthanized 28 days postoperatively. The right tibia was used for removal torque and RT-PCR, while the left tibia was collected for micro-CT and laser confocal microscopy. (3) Results: Functionalization with Scl-Ab significantly increased the gene expression of bone markers, especially ALP, in the SHAM Scl-Ab group compared to the other groups (<i>p</i> < 0.05). (4) Conclusions: Some parameters of this study indicate that implants functionalized with anti-sclerostin bone anabolic drug enhance peri-implant bone repair, especially in healthy rats. However, more studies must be carried out to confirm the therapeutic benefits of this approach.https://www.mdpi.com/2306-5354/12/4/358bonedental implantsosseointegrationosteoporosisrattibia |
| spellingShingle | Pedro Henrique Silva Gomes-Ferreira Paula Buzo Frigério Nathália Dantas Duarte Juliana de Moura Naara Gabriela Monteiro André Luis da Silva Fabris Roberta Okamoto Evaluation of Peri-Implant Bone Repair in Ovariectomized Rats Submitted to the Implant Placement Functionalized with Anti-Sclerostin Bioengineering bone dental implants osseointegration osteoporosis rat tibia |
| title | Evaluation of Peri-Implant Bone Repair in Ovariectomized Rats Submitted to the Implant Placement Functionalized with Anti-Sclerostin |
| title_full | Evaluation of Peri-Implant Bone Repair in Ovariectomized Rats Submitted to the Implant Placement Functionalized with Anti-Sclerostin |
| title_fullStr | Evaluation of Peri-Implant Bone Repair in Ovariectomized Rats Submitted to the Implant Placement Functionalized with Anti-Sclerostin |
| title_full_unstemmed | Evaluation of Peri-Implant Bone Repair in Ovariectomized Rats Submitted to the Implant Placement Functionalized with Anti-Sclerostin |
| title_short | Evaluation of Peri-Implant Bone Repair in Ovariectomized Rats Submitted to the Implant Placement Functionalized with Anti-Sclerostin |
| title_sort | evaluation of peri implant bone repair in ovariectomized rats submitted to the implant placement functionalized with anti sclerostin |
| topic | bone dental implants osseointegration osteoporosis rat tibia |
| url | https://www.mdpi.com/2306-5354/12/4/358 |
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