Solute Energetics in Aqueous Xanthan Gum Solutions: What Can Be Learned from a Fluorescent Probe?

Xanthan gum (XG) is a well-known anionic polysaccharide that finds broad application in the food and petroleum industries because of its ability to enhance solution viscosity at low concentrations and moderate temperatures. The aim of this work was to use the solvation probe coumarin 153 (C153) to c...

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Main Authors: Mark P. Heitz, Emmanuel M. Nsengiyumva, Paschalis Alexandridis
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Polysaccharides
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Online Access:https://www.mdpi.com/2673-4176/5/4/55
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author Mark P. Heitz
Emmanuel M. Nsengiyumva
Paschalis Alexandridis
author_facet Mark P. Heitz
Emmanuel M. Nsengiyumva
Paschalis Alexandridis
author_sort Mark P. Heitz
collection DOAJ
description Xanthan gum (XG) is a well-known anionic polysaccharide that finds broad application in the food and petroleum industries because of its ability to enhance solution viscosity at low concentrations and moderate temperatures. The aim of this work was to use the solvation probe coumarin 153 (C153) to characterize changes in the xanthan gum (XG) solution microstructure as a function of XG concentration and temperature from the perspective of a dissolved solute molecule. We established the utility of C153 fluorescence to track solution changes for XG concentrations that span the transition region from a dilute to a semi-dilute solution, defined by the xanthan gum overlap concentration, C*~0.02 g/dL. The temperature was varied from 293 to 353 K to probe solution conditions wherein XG has been reported to undergo a structural change from helix to random coil conformation, the details of which are still under debate. While C153 fluorescence does not elucidate direct structural information, the emission response is a simple means by which changes in aqueous XG solution can be identified. C153 spectroscopy is observed to correlate with XG conformational changes, as reported in the literature.
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spelling doaj-art-0164d2cdba5a4b448096d4209d6f73352025-08-20T02:01:09ZengMDPI AGPolysaccharides2673-41762024-12-015489291010.3390/polysaccharides5040055Solute Energetics in Aqueous Xanthan Gum Solutions: What Can Be Learned from a Fluorescent Probe?Mark P. Heitz0Emmanuel M. Nsengiyumva1Paschalis Alexandridis2Department of Chemistry and Biochemistry, The State University of New York (SUNY) Brockport, Brockport, NY 14420-2971, USADepartment of Chemistry and Biochemistry, The State University of New York (SUNY) Brockport, Brockport, NY 14420-2971, USADepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York (SUNY), Buffalo, NY 14260-4200, USAXanthan gum (XG) is a well-known anionic polysaccharide that finds broad application in the food and petroleum industries because of its ability to enhance solution viscosity at low concentrations and moderate temperatures. The aim of this work was to use the solvation probe coumarin 153 (C153) to characterize changes in the xanthan gum (XG) solution microstructure as a function of XG concentration and temperature from the perspective of a dissolved solute molecule. We established the utility of C153 fluorescence to track solution changes for XG concentrations that span the transition region from a dilute to a semi-dilute solution, defined by the xanthan gum overlap concentration, C*~0.02 g/dL. The temperature was varied from 293 to 353 K to probe solution conditions wherein XG has been reported to undergo a structural change from helix to random coil conformation, the details of which are still under debate. While C153 fluorescence does not elucidate direct structural information, the emission response is a simple means by which changes in aqueous XG solution can be identified. C153 spectroscopy is observed to correlate with XG conformational changes, as reported in the literature.https://www.mdpi.com/2673-4176/5/4/55biopolymerpolysaccharidepolyelectrolytexanthan gumfluorescence spectroscopyabsorbance spectroscopy
spellingShingle Mark P. Heitz
Emmanuel M. Nsengiyumva
Paschalis Alexandridis
Solute Energetics in Aqueous Xanthan Gum Solutions: What Can Be Learned from a Fluorescent Probe?
Polysaccharides
biopolymer
polysaccharide
polyelectrolyte
xanthan gum
fluorescence spectroscopy
absorbance spectroscopy
title Solute Energetics in Aqueous Xanthan Gum Solutions: What Can Be Learned from a Fluorescent Probe?
title_full Solute Energetics in Aqueous Xanthan Gum Solutions: What Can Be Learned from a Fluorescent Probe?
title_fullStr Solute Energetics in Aqueous Xanthan Gum Solutions: What Can Be Learned from a Fluorescent Probe?
title_full_unstemmed Solute Energetics in Aqueous Xanthan Gum Solutions: What Can Be Learned from a Fluorescent Probe?
title_short Solute Energetics in Aqueous Xanthan Gum Solutions: What Can Be Learned from a Fluorescent Probe?
title_sort solute energetics in aqueous xanthan gum solutions what can be learned from a fluorescent probe
topic biopolymer
polysaccharide
polyelectrolyte
xanthan gum
fluorescence spectroscopy
absorbance spectroscopy
url https://www.mdpi.com/2673-4176/5/4/55
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AT emmanuelmnsengiyumva soluteenergeticsinaqueousxanthangumsolutionswhatcanbelearnedfromafluorescentprobe
AT paschalisalexandridis soluteenergeticsinaqueousxanthangumsolutionswhatcanbelearnedfromafluorescentprobe