A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaques
The current study aims to develop a safe and highly immunogenic COVID-19 vaccine. The novel combination of a DNA vaccine encoding the full-length Spike (S) protein of SARS-CoV-2 and a recombinant S1 protein vaccine induced high level neutralizing antibody and T cell immune responses in both small an...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2021.1887767 |
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| author | Yuzhong Li Yanwei Bi Hongjian Xiao Yueting Yao Xiaojuan Liu Zhengrong Hu Jinmei Duan Yaoyun Yang Zhihua Li Yadong Li Heng Zhang Chen Ding Jianbo Yang Haiwei Li Zhanlong He Longding Liu Guangnan Hu Shuying Liu Yanchun Che Shixia Wang Qihan Li Shan Lu Wei Cun |
| author_facet | Yuzhong Li Yanwei Bi Hongjian Xiao Yueting Yao Xiaojuan Liu Zhengrong Hu Jinmei Duan Yaoyun Yang Zhihua Li Yadong Li Heng Zhang Chen Ding Jianbo Yang Haiwei Li Zhanlong He Longding Liu Guangnan Hu Shuying Liu Yanchun Che Shixia Wang Qihan Li Shan Lu Wei Cun |
| author_sort | Yuzhong Li |
| collection | DOAJ |
| description | The current study aims to develop a safe and highly immunogenic COVID-19 vaccine. The novel combination of a DNA vaccine encoding the full-length Spike (S) protein of SARS-CoV-2 and a recombinant S1 protein vaccine induced high level neutralizing antibody and T cell immune responses in both small and large animal models. More significantly, the co-delivery of DNA and protein components at the same time elicited full protection against intratracheal challenge of SARS-CoV-2 viruses in immunized rhesus macaques. As both DNA and protein vaccines have been proven safe in previous human studies, and DNA vaccines are capable of eliciting germinal center B cell development, which is critical for high-affinity memory B cell responses, the DNA and protein co-delivery vaccine approach has great potential to serve as a safe and effective approach to develop COVID-19 vaccines that provide long-term protection. |
| format | Article |
| id | doaj-art-01599f6a10234e47bc9e6bc3c3b37d22 |
| institution | Kabale University |
| issn | 2222-1751 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-01599f6a10234e47bc9e6bc3c3b37d222025-08-20T03:49:21ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512021-01-0110134235510.1080/22221751.2021.1887767A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaquesYuzhong Li0Yanwei Bi1Hongjian Xiao2Yueting Yao3Xiaojuan Liu4Zhengrong Hu5Jinmei Duan6Yaoyun Yang7Zhihua Li8Yadong Li9Heng Zhang10Chen Ding11Jianbo Yang12Haiwei Li13Zhanlong He14Longding Liu15Guangnan Hu16Shuying Liu17Yanchun Che18Shixia Wang19Qihan Li20Shan Lu21Wei Cun22Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USASYL-Consulting, Thousand Oaks, CA, USAInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USAInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USAInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of ChinaThe current study aims to develop a safe and highly immunogenic COVID-19 vaccine. The novel combination of a DNA vaccine encoding the full-length Spike (S) protein of SARS-CoV-2 and a recombinant S1 protein vaccine induced high level neutralizing antibody and T cell immune responses in both small and large animal models. More significantly, the co-delivery of DNA and protein components at the same time elicited full protection against intratracheal challenge of SARS-CoV-2 viruses in immunized rhesus macaques. As both DNA and protein vaccines have been proven safe in previous human studies, and DNA vaccines are capable of eliciting germinal center B cell development, which is critical for high-affinity memory B cell responses, the DNA and protein co-delivery vaccine approach has great potential to serve as a safe and effective approach to develop COVID-19 vaccines that provide long-term protection.https://www.tandfonline.com/doi/10.1080/22221751.2021.1887767SARS-CoV-2spike glycoproteinDNA vaccineprotein vaccinenon-human primate |
| spellingShingle | Yuzhong Li Yanwei Bi Hongjian Xiao Yueting Yao Xiaojuan Liu Zhengrong Hu Jinmei Duan Yaoyun Yang Zhihua Li Yadong Li Heng Zhang Chen Ding Jianbo Yang Haiwei Li Zhanlong He Longding Liu Guangnan Hu Shuying Liu Yanchun Che Shixia Wang Qihan Li Shan Lu Wei Cun A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaques Emerging Microbes and Infections SARS-CoV-2 spike glycoprotein DNA vaccine protein vaccine non-human primate |
| title | A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaques |
| title_full | A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaques |
| title_fullStr | A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaques |
| title_full_unstemmed | A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaques |
| title_short | A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaques |
| title_sort | novel dna and protein combination covid 19 vaccine formulation provides full protection against sars cov 2 in rhesus macaques |
| topic | SARS-CoV-2 spike glycoprotein DNA vaccine protein vaccine non-human primate |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2021.1887767 |
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