Recruitment of Perisomatic Inhibition during Spontaneous Hippocampal Activity In Vitro.
It was recently shown that perisomatic GABAergic inhibitory postsynaptic potentials (IPSPs) originating from basket and chandelier cells can be recorded as population IPSPs from the hippocampal pyramidal layer using extracellular electrodes (eIPSPs). Taking advantage of this approach, we have invest...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0066509&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850224576715292672 |
|---|---|
| author | Anna Beyeler Aude Retailleau Colin Molter Amine Mehidi Janos Szabadics Xavier Leinekugel |
| author_facet | Anna Beyeler Aude Retailleau Colin Molter Amine Mehidi Janos Szabadics Xavier Leinekugel |
| author_sort | Anna Beyeler |
| collection | DOAJ |
| description | It was recently shown that perisomatic GABAergic inhibitory postsynaptic potentials (IPSPs) originating from basket and chandelier cells can be recorded as population IPSPs from the hippocampal pyramidal layer using extracellular electrodes (eIPSPs). Taking advantage of this approach, we have investigated the recruitment of perisomatic inhibition during spontaneous hippocampal activity in vitro. Combining intracellular and extracellular recordings from pyramidal cells and interneurons, we confirm that inhibitory signals generated by basket cells can be recorded extracellularly, but our results suggest that, during spontaneous activity, eIPSPs are mostly confined to the CA3 rather than CA1 region. CA3 eIPSPs produced the powerful time-locked inhibition of multi-unit activity expected from perisomatic inhibition. Analysis of the temporal dynamics of spike discharges relative to eIPSPs suggests significant but moderate recruitment of excitatory and inhibitory neurons within the CA3 network on a 10 ms time scale, within which neurons recruit each other through recurrent collaterals and trigger powerful feedback inhibition. Such quantified parameters of neuronal interactions in the hippocampal network may serve as a basis for future characterisation of pathological conditions potentially affecting the interactions between excitation and inhibition in this circuit. |
| format | Article |
| id | doaj-art-0150da7a8aed4e22a1b61368458fd5f2 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-0150da7a8aed4e22a1b61368458fd5f22025-08-20T02:05:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6650910.1371/journal.pone.0066509Recruitment of Perisomatic Inhibition during Spontaneous Hippocampal Activity In Vitro.Anna BeyelerAude RetailleauColin MolterAmine MehidiJanos SzabadicsXavier LeinekugelIt was recently shown that perisomatic GABAergic inhibitory postsynaptic potentials (IPSPs) originating from basket and chandelier cells can be recorded as population IPSPs from the hippocampal pyramidal layer using extracellular electrodes (eIPSPs). Taking advantage of this approach, we have investigated the recruitment of perisomatic inhibition during spontaneous hippocampal activity in vitro. Combining intracellular and extracellular recordings from pyramidal cells and interneurons, we confirm that inhibitory signals generated by basket cells can be recorded extracellularly, but our results suggest that, during spontaneous activity, eIPSPs are mostly confined to the CA3 rather than CA1 region. CA3 eIPSPs produced the powerful time-locked inhibition of multi-unit activity expected from perisomatic inhibition. Analysis of the temporal dynamics of spike discharges relative to eIPSPs suggests significant but moderate recruitment of excitatory and inhibitory neurons within the CA3 network on a 10 ms time scale, within which neurons recruit each other through recurrent collaterals and trigger powerful feedback inhibition. Such quantified parameters of neuronal interactions in the hippocampal network may serve as a basis for future characterisation of pathological conditions potentially affecting the interactions between excitation and inhibition in this circuit.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0066509&type=printable |
| spellingShingle | Anna Beyeler Aude Retailleau Colin Molter Amine Mehidi Janos Szabadics Xavier Leinekugel Recruitment of Perisomatic Inhibition during Spontaneous Hippocampal Activity In Vitro. PLoS ONE |
| title | Recruitment of Perisomatic Inhibition during Spontaneous Hippocampal Activity In Vitro. |
| title_full | Recruitment of Perisomatic Inhibition during Spontaneous Hippocampal Activity In Vitro. |
| title_fullStr | Recruitment of Perisomatic Inhibition during Spontaneous Hippocampal Activity In Vitro. |
| title_full_unstemmed | Recruitment of Perisomatic Inhibition during Spontaneous Hippocampal Activity In Vitro. |
| title_short | Recruitment of Perisomatic Inhibition during Spontaneous Hippocampal Activity In Vitro. |
| title_sort | recruitment of perisomatic inhibition during spontaneous hippocampal activity in vitro |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0066509&type=printable |
| work_keys_str_mv | AT annabeyeler recruitmentofperisomaticinhibitionduringspontaneoushippocampalactivityinvitro AT auderetailleau recruitmentofperisomaticinhibitionduringspontaneoushippocampalactivityinvitro AT colinmolter recruitmentofperisomaticinhibitionduringspontaneoushippocampalactivityinvitro AT aminemehidi recruitmentofperisomaticinhibitionduringspontaneoushippocampalactivityinvitro AT janosszabadics recruitmentofperisomaticinhibitionduringspontaneoushippocampalactivityinvitro AT xavierleinekugel recruitmentofperisomaticinhibitionduringspontaneoushippocampalactivityinvitro |