Systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes
Abstract To characterize the impact of gut microbiota on host metabolism, we investigated the multicompartmental metabolic profiles of a conventional mouse strain (C3H/HeJ) (n=5) and its germ‐free (GF) equivalent (n=5). We confirm that the microbiome strongly impacts on the metabolism of bile acids...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2008-10-01
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| Series: | Molecular Systems Biology |
| Online Access: | https://doi.org/10.1038/msb.2008.56 |
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| _version_ | 1849225739206918144 |
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| author | Sandrine P Claus Tsz M Tsang Yulan Wang Olivier Cloarec Eleni Skordi François‐Pierre Martin Serge Rezzi Alastair Ross Sunil Kochhar Elaine Holmes Jeremy K Nicholson |
| author_facet | Sandrine P Claus Tsz M Tsang Yulan Wang Olivier Cloarec Eleni Skordi François‐Pierre Martin Serge Rezzi Alastair Ross Sunil Kochhar Elaine Holmes Jeremy K Nicholson |
| author_sort | Sandrine P Claus |
| collection | DOAJ |
| description | Abstract To characterize the impact of gut microbiota on host metabolism, we investigated the multicompartmental metabolic profiles of a conventional mouse strain (C3H/HeJ) (n=5) and its germ‐free (GF) equivalent (n=5). We confirm that the microbiome strongly impacts on the metabolism of bile acids through the enterohepatic cycle and gut metabolism (higher levels of phosphocholine and glycine in GF liver and marked higher levels of bile acids in three gut compartments). Furthermore we demonstrate that (1) well‐defined metabolic differences exist in all examined compartments between the metabotypes of GF and conventional mice: bacterial co‐metabolic products such as hippurate (urine) and 5‐aminovalerate (colon epithelium) were found at reduced concentrations, whereas raffinose was only detected in GF colonic profiles. (2) The microbiome also influences kidney homeostasis with elevated levels of key cell volume regulators (betaine, choline, myo‐inositol and so on) observed in GF kidneys. (3) Gut microbiota modulate metabotype expression at both local (gut) and global (biofluids, kidney, liver) system levels and hence influence the responses to a variety of dietary modulation and drug exposures relevant to personalized health‐care investigations. |
| format | Article |
| id | doaj-art-014e87089ee643779a114e7f7f4c4d53 |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2008-10-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-014e87089ee643779a114e7f7f4c4d532025-08-24T12:01:31ZengSpringer NatureMolecular Systems Biology1744-42922008-10-014111410.1038/msb.2008.56Systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypesSandrine P Claus0Tsz M Tsang1Yulan Wang2Olivier Cloarec3Eleni Skordi4François‐Pierre Martin5Serge Rezzi6Alastair Ross7Sunil Kochhar8Elaine Holmes9Jeremy K Nicholson10SORA Division, Department of Biomolecular Medicine, Imperial College LondonSORA Division, Department of Biomolecular Medicine, Imperial College LondonState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, The Chinese Academy of SciencesSORA Division, Department of Biomolecular Medicine, Imperial College LondonSORA Division, Department of Biomolecular Medicine, Imperial College LondonBioAnalytical Science Department, Nestlé Research CenterBioAnalytical Science Department, Nestlé Research CenterBioAnalytical Science Department, Nestlé Research CenterBioAnalytical Science Department, Nestlé Research CenterSORA Division, Department of Biomolecular Medicine, Imperial College LondonSORA Division, Department of Biomolecular Medicine, Imperial College LondonAbstract To characterize the impact of gut microbiota on host metabolism, we investigated the multicompartmental metabolic profiles of a conventional mouse strain (C3H/HeJ) (n=5) and its germ‐free (GF) equivalent (n=5). We confirm that the microbiome strongly impacts on the metabolism of bile acids through the enterohepatic cycle and gut metabolism (higher levels of phosphocholine and glycine in GF liver and marked higher levels of bile acids in three gut compartments). Furthermore we demonstrate that (1) well‐defined metabolic differences exist in all examined compartments between the metabotypes of GF and conventional mice: bacterial co‐metabolic products such as hippurate (urine) and 5‐aminovalerate (colon epithelium) were found at reduced concentrations, whereas raffinose was only detected in GF colonic profiles. (2) The microbiome also influences kidney homeostasis with elevated levels of key cell volume regulators (betaine, choline, myo‐inositol and so on) observed in GF kidneys. (3) Gut microbiota modulate metabotype expression at both local (gut) and global (biofluids, kidney, liver) system levels and hence influence the responses to a variety of dietary modulation and drug exposures relevant to personalized health‐care investigations.https://doi.org/10.1038/msb.2008.56 |
| spellingShingle | Sandrine P Claus Tsz M Tsang Yulan Wang Olivier Cloarec Eleni Skordi François‐Pierre Martin Serge Rezzi Alastair Ross Sunil Kochhar Elaine Holmes Jeremy K Nicholson Systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes Molecular Systems Biology |
| title | Systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes |
| title_full | Systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes |
| title_fullStr | Systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes |
| title_full_unstemmed | Systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes |
| title_short | Systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes |
| title_sort | systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes |
| url | https://doi.org/10.1038/msb.2008.56 |
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