CD206+ Cell Number Differentiates Influenza A (H1N1)pdm09 from Seasonal Influenza A Virus in Fatal Cases
In 2009, a new influenza A (H1N1) virus affected many persons around the world. There is an urgent need for finding biomarkers to distinguish between influenza A (H1N1)pdm09 and seasonal influenza virus. We investigated these possible biomarkers in the lung of fatal cases of confirmed influenza A (H...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/921054 |
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| author | Heidi G. Rodriguez-Ramirez Mario C. Salinas-Carmona Oralia Barboza-Quintana Americo Melo-de la Garza Luis Angel Ceceñas-Falcon Lilia M. Rangel-Martinez Adrian G. Rosas-Taraco |
| author_facet | Heidi G. Rodriguez-Ramirez Mario C. Salinas-Carmona Oralia Barboza-Quintana Americo Melo-de la Garza Luis Angel Ceceñas-Falcon Lilia M. Rangel-Martinez Adrian G. Rosas-Taraco |
| author_sort | Heidi G. Rodriguez-Ramirez |
| collection | DOAJ |
| description | In 2009, a new influenza A (H1N1) virus affected many persons around the world. There is an urgent need for finding biomarkers to distinguish between influenza A (H1N1)pdm09 and seasonal influenza virus. We investigated these possible biomarkers in the lung of fatal cases of confirmed influenza A (H1N1)pdm09. Cytokines (inflammatory and anti-inflammatory) and cellular markers (macrophages and lymphocytes subpopulation markers) were analyzed in lung tissue from both influenza A (H1N1)pdm09 and seasonal influenza virus. High levels of IL-17, IFN-γ, and TNF-α positive cells were identical in lung tissue from the influenza A (H1N1)pdm09 and seasonal cases when compared with healthy lung tissue (P<0.05). Increased IL-4+ cells, and CD4+ and CD14+ cells were also found in high levels in both influenza A (H1N1)pdm09 and seasonal influenza virus (P<0.05). Low levels of CD206+ cells (marker of alternatively activated macrophages marker in lung) were found in influenza A (H1N1)pdm09 when compared with seasonal influenza virus (P<0.05), and the ratio of CD206/CD14+ cells was 2.5-fold higher in seasonal and noninfluenza group compared with influenza A (H1N1)pdm09 (P<0.05). In conclusion, CD206+ cells differentiate between influenza A (H1N1)pdm09 and seasonal influenza virus in lung tissue of fatal cases. |
| format | Article |
| id | doaj-art-014aa3d461434a759dc3ef4a7941edd5 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-014aa3d461434a759dc3ef4a7941edd52025-08-20T02:01:34ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/921054921054CD206+ Cell Number Differentiates Influenza A (H1N1)pdm09 from Seasonal Influenza A Virus in Fatal CasesHeidi G. Rodriguez-Ramirez0Mario C. Salinas-Carmona1Oralia Barboza-Quintana2Americo Melo-de la Garza3Luis Angel Ceceñas-Falcon4Lilia M. Rangel-Martinez5Adrian G. Rosas-Taraco6Department of Immunology, School of Medicine and University Hospital, Universidad Autonoma de Nuevo Leon (UANL), Gonzalitos 235 Norte, Mitras Centro, 64460 Monterrey, NL, MexicoDepartment of Immunology, School of Medicine and University Hospital, Universidad Autonoma de Nuevo Leon (UANL), Gonzalitos 235 Norte, Mitras Centro, 64460 Monterrey, NL, MexicoServicio de Anatomia Patologica y Citopatologia, School of Medicine and University Hospital, Universidad Autonoma de Nuevo Leon (UANL), Monterrey, NL, MexicoServicio de Anatomia Patologica y Citopatologia, School of Medicine and University Hospital, Universidad Autonoma de Nuevo Leon (UANL), Monterrey, NL, MexicoServicio de Anatomia Patologica y Citopatologia, School of Medicine and University Hospital, Universidad Autonoma de Nuevo Leon (UANL), Monterrey, NL, MexicoDepartamento de Anatomia Patologica, Instituto Mexicano del Seguro Social (IMSS), HGZ No. 6, San Nicolas de los Garza, NL, MexicoDepartment of Immunology, School of Medicine and University Hospital, Universidad Autonoma de Nuevo Leon (UANL), Gonzalitos 235 Norte, Mitras Centro, 64460 Monterrey, NL, MexicoIn 2009, a new influenza A (H1N1) virus affected many persons around the world. There is an urgent need for finding biomarkers to distinguish between influenza A (H1N1)pdm09 and seasonal influenza virus. We investigated these possible biomarkers in the lung of fatal cases of confirmed influenza A (H1N1)pdm09. Cytokines (inflammatory and anti-inflammatory) and cellular markers (macrophages and lymphocytes subpopulation markers) were analyzed in lung tissue from both influenza A (H1N1)pdm09 and seasonal influenza virus. High levels of IL-17, IFN-γ, and TNF-α positive cells were identical in lung tissue from the influenza A (H1N1)pdm09 and seasonal cases when compared with healthy lung tissue (P<0.05). Increased IL-4+ cells, and CD4+ and CD14+ cells were also found in high levels in both influenza A (H1N1)pdm09 and seasonal influenza virus (P<0.05). Low levels of CD206+ cells (marker of alternatively activated macrophages marker in lung) were found in influenza A (H1N1)pdm09 when compared with seasonal influenza virus (P<0.05), and the ratio of CD206/CD14+ cells was 2.5-fold higher in seasonal and noninfluenza group compared with influenza A (H1N1)pdm09 (P<0.05). In conclusion, CD206+ cells differentiate between influenza A (H1N1)pdm09 and seasonal influenza virus in lung tissue of fatal cases.http://dx.doi.org/10.1155/2014/921054 |
| spellingShingle | Heidi G. Rodriguez-Ramirez Mario C. Salinas-Carmona Oralia Barboza-Quintana Americo Melo-de la Garza Luis Angel Ceceñas-Falcon Lilia M. Rangel-Martinez Adrian G. Rosas-Taraco CD206+ Cell Number Differentiates Influenza A (H1N1)pdm09 from Seasonal Influenza A Virus in Fatal Cases Mediators of Inflammation |
| title | CD206+ Cell Number Differentiates Influenza A (H1N1)pdm09 from Seasonal Influenza A Virus in Fatal Cases |
| title_full | CD206+ Cell Number Differentiates Influenza A (H1N1)pdm09 from Seasonal Influenza A Virus in Fatal Cases |
| title_fullStr | CD206+ Cell Number Differentiates Influenza A (H1N1)pdm09 from Seasonal Influenza A Virus in Fatal Cases |
| title_full_unstemmed | CD206+ Cell Number Differentiates Influenza A (H1N1)pdm09 from Seasonal Influenza A Virus in Fatal Cases |
| title_short | CD206+ Cell Number Differentiates Influenza A (H1N1)pdm09 from Seasonal Influenza A Virus in Fatal Cases |
| title_sort | cd206 cell number differentiates influenza a h1n1 pdm09 from seasonal influenza a virus in fatal cases |
| url | http://dx.doi.org/10.1155/2014/921054 |
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