The assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain WIBP-MPXV-001 in China
Since May 2022, human mpox cases have increased unexpectedly in non-endemic countries. The first imported case of human mpox in Hong Kong was reported in September 2022. Here we report the isolation and identification of MPXV from the vesicle swabs of this patient. In this research, the vesicle swab...
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Taylor & Francis Group
2023-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2023.2208682 |
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| author | Yan Zeng Xiaoke Liu Yuwei Li Jia Lu Qin Wu Demiao Dan Shiyun Lv Fei Xia Chunxia Hu Jiali Li Hao Zhang Hongqiao Du Rui Jia Kai Duan Zejun Wang Xinguo Li Xiaoming Yang |
| author_facet | Yan Zeng Xiaoke Liu Yuwei Li Jia Lu Qin Wu Demiao Dan Shiyun Lv Fei Xia Chunxia Hu Jiali Li Hao Zhang Hongqiao Du Rui Jia Kai Duan Zejun Wang Xinguo Li Xiaoming Yang |
| author_sort | Yan Zeng |
| collection | DOAJ |
| description | Since May 2022, human mpox cases have increased unexpectedly in non-endemic countries. The first imported case of human mpox in Hong Kong was reported in September 2022. Here we report the isolation and identification of MPXV from the vesicle swabs of this patient. In this research, the vesicle swabs were inoculated in Vero and Vero E6 cells. In addition to observing cytopathic effects (CPEs) in Vero or Vero E6 cells, the isolated virus was identified as mpox virus (MPXV) using quantitative Real-Time PCR (RT–PCR), transmission electron microscopy, and high-throughput sequencing. The experiment also assessed the cross-protective efficacy of sera from the smallpox vaccinated population and preliminarily assessed the inhibitory effect of anti-smallpox virus drugs against MPXV. CPEs can be observed on Vero E6 cells at 24 h and Vero cells at 48 h. The virus particles could be observed by transmission electron microscope, showing typical orthopoxvirus morphology. In addition, F3L and ATI genes which from MPXV A39R, B2R, HA genes which from orthopoxvirus were confirmed by conventional PCR and Sanger sequencing. The next generation sequencing (NGS) suggests that the MPXV strain belongs to B.1 branch of the West African linage, and has a high identity with the sequence of the 2022 ongoing outbreak. PRNT50 results showed that 26.7% of sera from individuals born before 1981 who had been immunized with smallpox were positive, but no MPXV-neutralizing antibodies were found in sera from individuals born later. All four anti-smallpox virus drugs evaluated demonstrated inhibition of mpox virus. |
| format | Article |
| id | doaj-art-013c694f81744bbc8f75bc12d51a766d |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
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| series | Emerging Microbes and Infections |
| spelling | doaj-art-013c694f81744bbc8f75bc12d51a766d2025-08-20T01:49:16ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112110.1080/22221751.2023.2208682The assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain WIBP-MPXV-001 in ChinaYan Zeng0Xiaoke Liu1Yuwei Li2Jia Lu3Qin Wu4Demiao Dan5Shiyun Lv6Fei Xia7Chunxia Hu8Jiali Li9Hao Zhang10Hongqiao Du11Rui Jia12Kai Duan13Zejun Wang14Xinguo Li15Xiaoming Yang16Wuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaChina National Biotec Group (CNBG), Beijing, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaWuhan Institute of Biological Products Co., Ltd., Wuhan, People’s Republic of ChinaChina National Biotec Group (CNBG), Beijing, People’s Republic of ChinaSince May 2022, human mpox cases have increased unexpectedly in non-endemic countries. The first imported case of human mpox in Hong Kong was reported in September 2022. Here we report the isolation and identification of MPXV from the vesicle swabs of this patient. In this research, the vesicle swabs were inoculated in Vero and Vero E6 cells. In addition to observing cytopathic effects (CPEs) in Vero or Vero E6 cells, the isolated virus was identified as mpox virus (MPXV) using quantitative Real-Time PCR (RT–PCR), transmission electron microscopy, and high-throughput sequencing. The experiment also assessed the cross-protective efficacy of sera from the smallpox vaccinated population and preliminarily assessed the inhibitory effect of anti-smallpox virus drugs against MPXV. CPEs can be observed on Vero E6 cells at 24 h and Vero cells at 48 h. The virus particles could be observed by transmission electron microscope, showing typical orthopoxvirus morphology. In addition, F3L and ATI genes which from MPXV A39R, B2R, HA genes which from orthopoxvirus were confirmed by conventional PCR and Sanger sequencing. The next generation sequencing (NGS) suggests that the MPXV strain belongs to B.1 branch of the West African linage, and has a high identity with the sequence of the 2022 ongoing outbreak. PRNT50 results showed that 26.7% of sera from individuals born before 1981 who had been immunized with smallpox were positive, but no MPXV-neutralizing antibodies were found in sera from individuals born later. All four anti-smallpox virus drugs evaluated demonstrated inhibition of mpox virus.https://www.tandfonline.com/doi/10.1080/22221751.2023.2208682IsolationidentificationMpox virusphylogeneticneutralizing antibodies |
| spellingShingle | Yan Zeng Xiaoke Liu Yuwei Li Jia Lu Qin Wu Demiao Dan Shiyun Lv Fei Xia Chunxia Hu Jiali Li Hao Zhang Hongqiao Du Rui Jia Kai Duan Zejun Wang Xinguo Li Xiaoming Yang The assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain WIBP-MPXV-001 in China Emerging Microbes and Infections Isolation identification Mpox virus phylogenetic neutralizing antibodies |
| title | The assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain WIBP-MPXV-001 in China |
| title_full | The assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain WIBP-MPXV-001 in China |
| title_fullStr | The assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain WIBP-MPXV-001 in China |
| title_full_unstemmed | The assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain WIBP-MPXV-001 in China |
| title_short | The assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain WIBP-MPXV-001 in China |
| title_sort | assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain wibp mpxv 001 in china |
| topic | Isolation identification Mpox virus phylogenetic neutralizing antibodies |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2023.2208682 |
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