Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry

Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry

Summary: Genetically encoded fluorescent sensors are powerful tools for tracking real-time neuromodulator dynamics. Here, we present a protocol to measure adenosine release using the G protein-coupled receptor activation-based adenosine (GRABAdo1.0m) sensor in the mouse hippocampus. We describe step...

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Bibliographic Details
Main Authors: Coralie Berthoux, Pablo E. Castillo, Kaoutsar Nasrallah
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Model Organisms
Neuroscience
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725004022
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