Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry
Summary: Genetically encoded fluorescent sensors are powerful tools for tracking real-time neuromodulator dynamics. Here, we present a protocol to measure adenosine release using the G protein-coupled receptor activation-based adenosine (GRABAdo1.0m) sensor in the mouse hippocampus. We describe step...
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| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725004022 |
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| author | Coralie Berthoux Pablo E. Castillo Kaoutsar Nasrallah |
| author_facet | Coralie Berthoux Pablo E. Castillo Kaoutsar Nasrallah |
| author_sort | Coralie Berthoux |
| collection | DOAJ |
| description | Summary: Genetically encoded fluorescent sensors are powerful tools for tracking real-time neuromodulator dynamics. Here, we present a protocol to measure adenosine release using the G protein-coupled receptor activation-based adenosine (GRABAdo1.0m) sensor in the mouse hippocampus. We describe steps for stereotaxic surgery, including virus injection and optic fiber implantation, ex vivo two-photon live imaging, and in vivo fiber photometry. We then detail histological procedures for experimental validation.For complete details on the use and execution of this protocol, please refer to Nasrallah et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-0139dd4f19324cf4a6d505b623a1d09c |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-0139dd4f19324cf4a6d505b623a1d09c2025-08-20T03:31:37ZengElsevierSTAR Protocols2666-16672025-09-016310399610.1016/j.xpro.2025.103996Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometryCoralie Berthoux0Pablo E. Castillo1Kaoutsar Nasrallah2Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA; Corresponding authorDominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA; Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA; Corresponding authorDominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA; Department of Biological Sciences, Fordham University, Bronx, New York, NY 10458, USA; Corresponding authorSummary: Genetically encoded fluorescent sensors are powerful tools for tracking real-time neuromodulator dynamics. Here, we present a protocol to measure adenosine release using the G protein-coupled receptor activation-based adenosine (GRABAdo1.0m) sensor in the mouse hippocampus. We describe steps for stereotaxic surgery, including virus injection and optic fiber implantation, ex vivo two-photon live imaging, and in vivo fiber photometry. We then detail histological procedures for experimental validation.For complete details on the use and execution of this protocol, please refer to Nasrallah et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725004022Model OrganismsNeuroscienceMolecular/Chemical Probes |
| spellingShingle | Coralie Berthoux Pablo E. Castillo Kaoutsar Nasrallah Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry STAR Protocols Model Organisms Neuroscience Molecular/Chemical Probes |
| title | Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry |
| title_full | Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry |
| title_fullStr | Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry |
| title_full_unstemmed | Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry |
| title_short | Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry |
| title_sort | protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor 2 photon live imaging and fiber photometry |
| topic | Model Organisms Neuroscience Molecular/Chemical Probes |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725004022 |
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