<em>Fasciola gigantica</em> Recombinant Abelson Tyrosine Protein Kinase (r<em>Fg</em>Abl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells
<i>Fasciola gigantica</i> can modulate host immune mechanisms through excretory–secretory products (ESP). As one of the components of ESP, it is unknown whether Abelson tyrosine protein kinase (Abl) is involved in parasite–host immune interaction. To investigate the immunoregulatory func...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/15/2/179 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849715618321793024 |
|---|---|
| author | Min Zhao Yu Zou Wanting Chen Dongqi Wu Chengjun Xian Haoqing Yang Jiacheng Tan Wenda Di Wende Wu Dongying Wang |
| author_facet | Min Zhao Yu Zou Wanting Chen Dongqi Wu Chengjun Xian Haoqing Yang Jiacheng Tan Wenda Di Wende Wu Dongying Wang |
| author_sort | Min Zhao |
| collection | DOAJ |
| description | <i>Fasciola gigantica</i> can modulate host immune mechanisms through excretory–secretory products (ESP). As one of the components of ESP, it is unknown whether Abelson tyrosine protein kinase (Abl) is involved in parasite–host immune interaction. To investigate the immunoregulatory function of Abl in <i>Fasciola gigantica</i>, we cloned and expressed the <i>Fasciola</i> gigantica Abl protein and assessed its effect on specific immune functions of buffalo peripheral blood mononuclear cells (PBMCs). Recombinant <i>F. gigantica</i> Abelson tyrosine protein kinase (r<i>Fg</i>Abl) was expressed in <i>Escherichia coli</i>. Western blot analysis was performed to assess the reactivity of anti-r<i>Fg</i>Abl antibodies with r<i>Fg</i>Abl, serum from <i>F. gigantica</i>-infected buffalo, and excretion and secretion products of <i>F. gigantica</i>. Immunohistochemical analysis was conducted to determine the localization of <i>Fg</i>Abl in tissues from larval stages and adult worms of <i>F. gigantica</i>. Furthermore, immunofluorescence analysis was utilized to evaluate the binding ability of the r<i>Fg</i>Abl protein to buffalo peripheral blood mononuclear cells (PBMCs), as well as to investigate the effects of varying concentrations of r<i>Fg</i>Abl protein (5, 10, 20, 40, and 80 μg/mL) on the functional responses of PBMCs. Anti-r<i>Fg</i>Abl antibodies specifically recognize r<i>Fg</i>Abl, serum from buffalo infected with <i>F. gigantica</i>, and <i>Fg</i>ESP. r<i>Fg</i>Abl is localized in the cecum and capsule of juvenile worms, as well as in the testis and viellaria of adult worms. Additionally, r<i>Fg</i>Abl enhances cell proliferation, migration, nitric oxide (NO) production, and phagocytosis, while also increasing the transcription levels of cytokines (IFN-γ, IL-12, TNF-α, IL-4, IL-10, and TGF-β). The results indicate that r<i>Fg</i>Abl can influence the immune function of PBMCs. Further investigation into the immunomodulatory properties of the r<i>Fg</i>Abl protein will enhance our understanding of the immune interaction mechanisms between trematodes and their hosts. |
| format | Article |
| id | doaj-art-013713aa47154964be8839b8ffbd8a43 |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-013713aa47154964be8839b8ffbd8a432025-08-20T03:13:18ZengMDPI AGAnimals2076-26152025-01-0115217910.3390/ani15020179<em>Fasciola gigantica</em> Recombinant Abelson Tyrosine Protein Kinase (r<em>Fg</em>Abl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear CellsMin Zhao0Yu Zou1Wanting Chen2Dongqi Wu3Chengjun Xian4Haoqing Yang5Jiacheng Tan6Wenda Di7Wende Wu8Dongying Wang9Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China<i>Fasciola gigantica</i> can modulate host immune mechanisms through excretory–secretory products (ESP). As one of the components of ESP, it is unknown whether Abelson tyrosine protein kinase (Abl) is involved in parasite–host immune interaction. To investigate the immunoregulatory function of Abl in <i>Fasciola gigantica</i>, we cloned and expressed the <i>Fasciola</i> gigantica Abl protein and assessed its effect on specific immune functions of buffalo peripheral blood mononuclear cells (PBMCs). Recombinant <i>F. gigantica</i> Abelson tyrosine protein kinase (r<i>Fg</i>Abl) was expressed in <i>Escherichia coli</i>. Western blot analysis was performed to assess the reactivity of anti-r<i>Fg</i>Abl antibodies with r<i>Fg</i>Abl, serum from <i>F. gigantica</i>-infected buffalo, and excretion and secretion products of <i>F. gigantica</i>. Immunohistochemical analysis was conducted to determine the localization of <i>Fg</i>Abl in tissues from larval stages and adult worms of <i>F. gigantica</i>. Furthermore, immunofluorescence analysis was utilized to evaluate the binding ability of the r<i>Fg</i>Abl protein to buffalo peripheral blood mononuclear cells (PBMCs), as well as to investigate the effects of varying concentrations of r<i>Fg</i>Abl protein (5, 10, 20, 40, and 80 μg/mL) on the functional responses of PBMCs. Anti-r<i>Fg</i>Abl antibodies specifically recognize r<i>Fg</i>Abl, serum from buffalo infected with <i>F. gigantica</i>, and <i>Fg</i>ESP. r<i>Fg</i>Abl is localized in the cecum and capsule of juvenile worms, as well as in the testis and viellaria of adult worms. Additionally, r<i>Fg</i>Abl enhances cell proliferation, migration, nitric oxide (NO) production, and phagocytosis, while also increasing the transcription levels of cytokines (IFN-γ, IL-12, TNF-α, IL-4, IL-10, and TGF-β). The results indicate that r<i>Fg</i>Abl can influence the immune function of PBMCs. Further investigation into the immunomodulatory properties of the r<i>Fg</i>Abl protein will enhance our understanding of the immune interaction mechanisms between trematodes and their hosts.https://www.mdpi.com/2076-2615/15/2/179<i>Fasciola gigantica</i>Abelson tyrosine protein kinasehost–parasite interactionsimmune regulation |
| spellingShingle | Min Zhao Yu Zou Wanting Chen Dongqi Wu Chengjun Xian Haoqing Yang Jiacheng Tan Wenda Di Wende Wu Dongying Wang <em>Fasciola gigantica</em> Recombinant Abelson Tyrosine Protein Kinase (r<em>Fg</em>Abl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells Animals <i>Fasciola gigantica</i> Abelson tyrosine protein kinase host–parasite interactions immune regulation |
| title | <em>Fasciola gigantica</em> Recombinant Abelson Tyrosine Protein Kinase (r<em>Fg</em>Abl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells |
| title_full | <em>Fasciola gigantica</em> Recombinant Abelson Tyrosine Protein Kinase (r<em>Fg</em>Abl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells |
| title_fullStr | <em>Fasciola gigantica</em> Recombinant Abelson Tyrosine Protein Kinase (r<em>Fg</em>Abl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells |
| title_full_unstemmed | <em>Fasciola gigantica</em> Recombinant Abelson Tyrosine Protein Kinase (r<em>Fg</em>Abl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells |
| title_short | <em>Fasciola gigantica</em> Recombinant Abelson Tyrosine Protein Kinase (r<em>Fg</em>Abl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells |
| title_sort | em fasciola gigantica em recombinant abelson tyrosine protein kinase r em fg em abl regulates various functions of buffalo peripheral blood mononuclear cells |
| topic | <i>Fasciola gigantica</i> Abelson tyrosine protein kinase host–parasite interactions immune regulation |
| url | https://www.mdpi.com/2076-2615/15/2/179 |
| work_keys_str_mv | AT minzhao emfasciolagiganticaemrecombinantabelsontyrosineproteinkinaseremfgemablregulatesvariousfunctionsofbuffaloperipheralbloodmononuclearcells AT yuzou emfasciolagiganticaemrecombinantabelsontyrosineproteinkinaseremfgemablregulatesvariousfunctionsofbuffaloperipheralbloodmononuclearcells AT wantingchen emfasciolagiganticaemrecombinantabelsontyrosineproteinkinaseremfgemablregulatesvariousfunctionsofbuffaloperipheralbloodmononuclearcells AT dongqiwu emfasciolagiganticaemrecombinantabelsontyrosineproteinkinaseremfgemablregulatesvariousfunctionsofbuffaloperipheralbloodmononuclearcells AT chengjunxian emfasciolagiganticaemrecombinantabelsontyrosineproteinkinaseremfgemablregulatesvariousfunctionsofbuffaloperipheralbloodmononuclearcells AT haoqingyang emfasciolagiganticaemrecombinantabelsontyrosineproteinkinaseremfgemablregulatesvariousfunctionsofbuffaloperipheralbloodmononuclearcells AT jiachengtan emfasciolagiganticaemrecombinantabelsontyrosineproteinkinaseremfgemablregulatesvariousfunctionsofbuffaloperipheralbloodmononuclearcells AT wendadi emfasciolagiganticaemrecombinantabelsontyrosineproteinkinaseremfgemablregulatesvariousfunctionsofbuffaloperipheralbloodmononuclearcells AT wendewu emfasciolagiganticaemrecombinantabelsontyrosineproteinkinaseremfgemablregulatesvariousfunctionsofbuffaloperipheralbloodmononuclearcells AT dongyingwang emfasciolagiganticaemrecombinantabelsontyrosineproteinkinaseremfgemablregulatesvariousfunctionsofbuffaloperipheralbloodmononuclearcells |