Aptamer-enhanced ultrasensitive electrochemical detection of HER-2 in breast cancer diagnosis using ZnO tetrapod-K4PTC nanohybrids
Abstract Breast cancer continues to be a severe global health issue, emphasizing the critical need for accurate and timely detection and screening of large numbers of patients. This ailment places an enormous strain on healthcare resources and poses severe mortality risks for women globally if not a...
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Nature Portfolio
2025-05-01
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| Online Access: | https://doi.org/10.1038/s41598-025-88335-3 |
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| author | Reema Rawat Souradeep Roy Tapas Goswami Fatemeh Sadat Mirsafi Mustafa Ismael Till Leissner Yogendra Kumar Mishra James McLaughlin Krishna Kant Ashish Mathur |
| author_facet | Reema Rawat Souradeep Roy Tapas Goswami Fatemeh Sadat Mirsafi Mustafa Ismael Till Leissner Yogendra Kumar Mishra James McLaughlin Krishna Kant Ashish Mathur |
| author_sort | Reema Rawat |
| collection | DOAJ |
| description | Abstract Breast cancer continues to be a severe global health issue, emphasizing the critical need for accurate and timely detection and screening of large numbers of patients. This ailment places an enormous strain on healthcare resources and poses severe mortality risks for women globally if not addressed promptly. Conventional diagnostic techniques face obstacles such as expensive apparatus, intricate procedures, and long duration in stratification. Therefore, the development of point-of-care (PoC) devices that enable early detection of breast cancer is of utmost importance. This research article focuses on the development of an electrochemical based aptasensor for the detection of Human Epidermal Growth Factor Receptor 2 (HER-2) using the novel nanohybrid substrate material, which consists of a 3D Zinc Oxide Tetrapods (ZnOT) and Potassium Perylene Tetra Carboxylate (K4PTC). The analytical performance of the bio-nanoelectrode for detecting HER-2 was assessed using Square Wave Voltammetry (SWV) analysis. The developed nano-biosensor exhibited linear response in the range from 1 fg/mL – 10 µg/mL, and the overall limit of detection and sensitivity of the developed sensor is observed around 0.58 fg/mL and 2.08 µA/fg/mL/mm2, respectively. The shelf life of the sensor substrate is above one month in normal storage conditions. This study offers a base for the development of an efficient and sensitive breast cancer biomarker sensing platform, which has the potential to be implemented at a low cost for society and perform screening testing for breast cancer management in rural areas. |
| format | Article |
| id | doaj-art-011afe82263241eaaba58882ebd741cc |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-011afe82263241eaaba58882ebd741cc2025-08-20T01:51:32ZengNature PortfolioScientific Reports2045-23222025-05-0115111110.1038/s41598-025-88335-3Aptamer-enhanced ultrasensitive electrochemical detection of HER-2 in breast cancer diagnosis using ZnO tetrapod-K4PTC nanohybridsReema Rawat0Souradeep Roy1Tapas Goswami2Fatemeh Sadat Mirsafi3Mustafa Ismael4Till Leissner5Yogendra Kumar Mishra6James McLaughlin7Krishna Kant8Ashish Mathur9Department of Allied Health Sciences, School of Health Sciences and Technology, UPESDepartment of Allied Health Sciences, School of Health Sciences and Technology, UPESDepartment of Chemistry, School of Advanced Engineering, UPESMads Clausen Institute, University of Southern Denmark, NanoSYDMiddle Technical UniversityMads Clausen Institute, University of Southern Denmark, NanoSYDMads Clausen Institute, University of Southern Denmark, NanoSYDSchool of Engineering, Ulster UniversityCINBIO, Universidade de VigoCentre for Interdisciplinary Research and Innovation (CIDRI), UPESAbstract Breast cancer continues to be a severe global health issue, emphasizing the critical need for accurate and timely detection and screening of large numbers of patients. This ailment places an enormous strain on healthcare resources and poses severe mortality risks for women globally if not addressed promptly. Conventional diagnostic techniques face obstacles such as expensive apparatus, intricate procedures, and long duration in stratification. Therefore, the development of point-of-care (PoC) devices that enable early detection of breast cancer is of utmost importance. This research article focuses on the development of an electrochemical based aptasensor for the detection of Human Epidermal Growth Factor Receptor 2 (HER-2) using the novel nanohybrid substrate material, which consists of a 3D Zinc Oxide Tetrapods (ZnOT) and Potassium Perylene Tetra Carboxylate (K4PTC). The analytical performance of the bio-nanoelectrode for detecting HER-2 was assessed using Square Wave Voltammetry (SWV) analysis. The developed nano-biosensor exhibited linear response in the range from 1 fg/mL – 10 µg/mL, and the overall limit of detection and sensitivity of the developed sensor is observed around 0.58 fg/mL and 2.08 µA/fg/mL/mm2, respectively. The shelf life of the sensor substrate is above one month in normal storage conditions. This study offers a base for the development of an efficient and sensitive breast cancer biomarker sensing platform, which has the potential to be implemented at a low cost for society and perform screening testing for breast cancer management in rural areas.https://doi.org/10.1038/s41598-025-88335-3Breast cancerHER-2Electrochemical biosensorZnOT-K4PTC nanohybridsAffordable healthcareEarly detection |
| spellingShingle | Reema Rawat Souradeep Roy Tapas Goswami Fatemeh Sadat Mirsafi Mustafa Ismael Till Leissner Yogendra Kumar Mishra James McLaughlin Krishna Kant Ashish Mathur Aptamer-enhanced ultrasensitive electrochemical detection of HER-2 in breast cancer diagnosis using ZnO tetrapod-K4PTC nanohybrids Scientific Reports Breast cancer HER-2 Electrochemical biosensor ZnOT-K4PTC nanohybrids Affordable healthcare Early detection |
| title | Aptamer-enhanced ultrasensitive electrochemical detection of HER-2 in breast cancer diagnosis using ZnO tetrapod-K4PTC nanohybrids |
| title_full | Aptamer-enhanced ultrasensitive electrochemical detection of HER-2 in breast cancer diagnosis using ZnO tetrapod-K4PTC nanohybrids |
| title_fullStr | Aptamer-enhanced ultrasensitive electrochemical detection of HER-2 in breast cancer diagnosis using ZnO tetrapod-K4PTC nanohybrids |
| title_full_unstemmed | Aptamer-enhanced ultrasensitive electrochemical detection of HER-2 in breast cancer diagnosis using ZnO tetrapod-K4PTC nanohybrids |
| title_short | Aptamer-enhanced ultrasensitive electrochemical detection of HER-2 in breast cancer diagnosis using ZnO tetrapod-K4PTC nanohybrids |
| title_sort | aptamer enhanced ultrasensitive electrochemical detection of her 2 in breast cancer diagnosis using zno tetrapod k4ptc nanohybrids |
| topic | Breast cancer HER-2 Electrochemical biosensor ZnOT-K4PTC nanohybrids Affordable healthcare Early detection |
| url | https://doi.org/10.1038/s41598-025-88335-3 |
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