Isolation and characterization of Acinetobacter baumannii from environmental waters in Dhaka City, Bangladesh

Abstract Acinetobacter baumannii, a gram-negative bacterium commonly associated with nosocomial infections, has been relatively unexplored in the environmental context. The present study was conducted in Dhaka City, Bangladesh, with the primary objective of isolating and characterizing A. baumannii...

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Main Authors: Mohammed Aziz Hossain, Nayara Noor E. Fatima, Jahid H. Tushar, Hasib Mahmud, Fahim Kabir Monjurul Haque
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Microbiology
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Online Access:https://doi.org/10.1186/s12866-025-04029-w
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author Mohammed Aziz Hossain
Nayara Noor E. Fatima
Jahid H. Tushar
Hasib Mahmud
Fahim Kabir Monjurul Haque
author_facet Mohammed Aziz Hossain
Nayara Noor E. Fatima
Jahid H. Tushar
Hasib Mahmud
Fahim Kabir Monjurul Haque
author_sort Mohammed Aziz Hossain
collection DOAJ
description Abstract Acinetobacter baumannii, a gram-negative bacterium commonly associated with nosocomial infections, has been relatively unexplored in the environmental context. The present study was conducted in Dhaka City, Bangladesh, with the primary objective of isolating and characterizing A. baumannii in environmental water sources. Surface water samples were collected from various water reservoirs to detect the presence of A. baumannii. Isolates were confirmed as A. baumannii using culture and PCR. Confirmed isolates were screened for antimicrobial susceptibility, antimicrobial resistance genes, and serum resistance. Results revealed that 32% of the water samples tested positive for A. baumannii. In total, 23 A. baumannii isolates were obtained. All isolates showed resistance to Cefepime. Varying degrees of resistance to other antibiotics were observed, and 56% showed resistance to the bactericidal effect of serum. This study underscored the remarkable adaptability of A. baumannii and its ability to flourish in diverse environmental conditions, highlighting public health concerns of increasing antibiotic resistant bacteria. The study concluded that, given the significance of effective infection control and sanitation and waste management measures, understanding the presence and behavior of A. baumannii in the environment is paramount. This study acts as the first report on environmental A. baumannii in Bangladesh and further research is warranted to elucidate the underlying mechanisms of antibiotic resistance and their implications for human health.
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spelling doaj-art-011af5c8053e4e7e987652447c10c2b82025-08-20T02:34:17ZengBMCBMC Microbiology1471-21802025-05-0125111110.1186/s12866-025-04029-wIsolation and characterization of Acinetobacter baumannii from environmental waters in Dhaka City, BangladeshMohammed Aziz Hossain0Nayara Noor E. Fatima1Jahid H. Tushar2Hasib Mahmud3Fahim Kabir Monjurul Haque4Biotechnology program, Department of Mathematics and Natural Sciences, BRAC UniversityMicrobiology Program, Department of Mathematics and Natural Sciences, BRAC UniversityMicrobiology Program, Department of Mathematics and Natural Sciences, BRAC UniversityMicrobiology Program, Department of Mathematics and Natural Sciences, BRAC UniversityMicrobiology Program, Department of Mathematics and Natural Sciences, BRAC UniversityAbstract Acinetobacter baumannii, a gram-negative bacterium commonly associated with nosocomial infections, has been relatively unexplored in the environmental context. The present study was conducted in Dhaka City, Bangladesh, with the primary objective of isolating and characterizing A. baumannii in environmental water sources. Surface water samples were collected from various water reservoirs to detect the presence of A. baumannii. Isolates were confirmed as A. baumannii using culture and PCR. Confirmed isolates were screened for antimicrobial susceptibility, antimicrobial resistance genes, and serum resistance. Results revealed that 32% of the water samples tested positive for A. baumannii. In total, 23 A. baumannii isolates were obtained. All isolates showed resistance to Cefepime. Varying degrees of resistance to other antibiotics were observed, and 56% showed resistance to the bactericidal effect of serum. This study underscored the remarkable adaptability of A. baumannii and its ability to flourish in diverse environmental conditions, highlighting public health concerns of increasing antibiotic resistant bacteria. The study concluded that, given the significance of effective infection control and sanitation and waste management measures, understanding the presence and behavior of A. baumannii in the environment is paramount. This study acts as the first report on environmental A. baumannii in Bangladesh and further research is warranted to elucidate the underlying mechanisms of antibiotic resistance and their implications for human health.https://doi.org/10.1186/s12866-025-04029-wEnvironmental Acinetobacter baumanniiAntibiotic resistanceSerum resistant Acinetobacter baumanniiAntibiotic Resistant Acinetobacter baumannii
spellingShingle Mohammed Aziz Hossain
Nayara Noor E. Fatima
Jahid H. Tushar
Hasib Mahmud
Fahim Kabir Monjurul Haque
Isolation and characterization of Acinetobacter baumannii from environmental waters in Dhaka City, Bangladesh
BMC Microbiology
Environmental Acinetobacter baumannii
Antibiotic resistance
Serum resistant Acinetobacter baumannii
Antibiotic Resistant Acinetobacter baumannii
title Isolation and characterization of Acinetobacter baumannii from environmental waters in Dhaka City, Bangladesh
title_full Isolation and characterization of Acinetobacter baumannii from environmental waters in Dhaka City, Bangladesh
title_fullStr Isolation and characterization of Acinetobacter baumannii from environmental waters in Dhaka City, Bangladesh
title_full_unstemmed Isolation and characterization of Acinetobacter baumannii from environmental waters in Dhaka City, Bangladesh
title_short Isolation and characterization of Acinetobacter baumannii from environmental waters in Dhaka City, Bangladesh
title_sort isolation and characterization of acinetobacter baumannii from environmental waters in dhaka city bangladesh
topic Environmental Acinetobacter baumannii
Antibiotic resistance
Serum resistant Acinetobacter baumannii
Antibiotic Resistant Acinetobacter baumannii
url https://doi.org/10.1186/s12866-025-04029-w
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