Characterization of carotenoid profiles and presence of functional markers in sub-tropical maize (Zea mays L.) inbred lines
Biofortification provides a sustainable, pragmatic strategy to address the lack of vitamin A and the associated health complications. The objectives of the study encompassed the quantification of the carotenoid content of 147 maize inbred lines, the identification of variable regions within the ZEP1...
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Czech Academy of Agricultural Sciences
2025-04-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
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| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-202502-0004_characterization-of-carotenoid-profiles-and-presence-of-functional-markers-in-sub-tropical-maize-zea-mays-l.php |
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| author | Peter Amoah Victor O. Adetimirin Bismark Anokye Oluyinka J. llesanmi Nnanna Unachukwu Elisabeth Ivania Aporva |
| author_facet | Peter Amoah Victor O. Adetimirin Bismark Anokye Oluyinka J. llesanmi Nnanna Unachukwu Elisabeth Ivania Aporva |
| author_sort | Peter Amoah |
| collection | DOAJ |
| description | Biofortification provides a sustainable, pragmatic strategy to address the lack of vitamin A and the associated health complications. The objectives of the study encompassed the quantification of the carotenoid content of 147 maize inbred lines, the identification of variable regions within the ZEP1 gene, the correlation of these observed variances in the presence of this gene with carotenoid content, and the identification of lines harbouring the favourable alleles of the crtRB1 gene. The observed correlations among the carotenoids synthesised by distinct branches of the biosynthetic process were both significant and positive. Utilising gel-based genotyping, 24 lines with contrasting carotenoid profiles were selected, evaluated and sequenced. Analysis of the variation in the sequence classified these lines based on their similarities to give 8 allele groups. The findings highlight that inbred lines both group 1 and group 8 exhibited significant associations with the carotenoid content of the lines. Specifically, ZEP1_7852, a discernible variation belonging to group 8, was found to be significantly associated with zeaxanthin content and total carotenoid content. Furthermore, 25 lines were found to have provitamin A content above 15 μg/g, harbouring the favourable alleles of the crtRB1 gene using KASP SNP zm0016. These lines can serve as parents for source populations and hybrids, leading to the further enhancement of provitamin A in maize. |
| format | Article |
| id | doaj-art-010aa4c6ad7c4bc0834e525649b9500a |
| institution | OA Journals |
| issn | 1212-1975 1805-9325 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj-art-010aa4c6ad7c4bc0834e525649b9500a2025-08-20T02:11:47ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252025-04-01612869910.17221/143/2024-CJGPBcjg-202502-0004Characterization of carotenoid profiles and presence of functional markers in sub-tropical maize (Zea mays L.) inbred linesPeter Amoah0https://orcid.org/0000-0002-6141-4974Victor O. Adetimirin1Bismark Anokye2Oluyinka J. llesanmi3Nnanna Unachukwu4Elisabeth Ivania Aporva5https://orcid.org/0009-0000-3764-8511Pan African University, Life and Earth Sciences Institute, Ibadan, NigeriaDepartment of Crop and Horticultural Sciences, University of Ibadan, Ibadan, Oyo State, NigeriaDepartment of Soil and Crop Sciences, Texas A&M University, College Station, USAInternational Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, NigeriaInternational Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, NigeriaPan African University, Life and Earth Sciences Institute, Ibadan, NigeriaBiofortification provides a sustainable, pragmatic strategy to address the lack of vitamin A and the associated health complications. The objectives of the study encompassed the quantification of the carotenoid content of 147 maize inbred lines, the identification of variable regions within the ZEP1 gene, the correlation of these observed variances in the presence of this gene with carotenoid content, and the identification of lines harbouring the favourable alleles of the crtRB1 gene. The observed correlations among the carotenoids synthesised by distinct branches of the biosynthetic process were both significant and positive. Utilising gel-based genotyping, 24 lines with contrasting carotenoid profiles were selected, evaluated and sequenced. Analysis of the variation in the sequence classified these lines based on their similarities to give 8 allele groups. The findings highlight that inbred lines both group 1 and group 8 exhibited significant associations with the carotenoid content of the lines. Specifically, ZEP1_7852, a discernible variation belonging to group 8, was found to be significantly associated with zeaxanthin content and total carotenoid content. Furthermore, 25 lines were found to have provitamin A content above 15 μg/g, harbouring the favourable alleles of the crtRB1 gene using KASP SNP zm0016. These lines can serve as parents for source populations and hybrids, leading to the further enhancement of provitamin A in maize.https://cjgpb.agriculturejournals.cz/artkey/cjg-202502-0004_characterization-of-carotenoid-profiles-and-presence-of-functional-markers-in-sub-tropical-maize-zea-mays-l.phpcarotenoidkaspprovitamin asub-tropical maize lineszep1 |
| spellingShingle | Peter Amoah Victor O. Adetimirin Bismark Anokye Oluyinka J. llesanmi Nnanna Unachukwu Elisabeth Ivania Aporva Characterization of carotenoid profiles and presence of functional markers in sub-tropical maize (Zea mays L.) inbred lines Czech Journal of Genetics and Plant Breeding carotenoid kasp provitamin a sub-tropical maize lines zep1 |
| title | Characterization of carotenoid profiles and presence of functional markers in sub-tropical maize (Zea mays L.) inbred lines |
| title_full | Characterization of carotenoid profiles and presence of functional markers in sub-tropical maize (Zea mays L.) inbred lines |
| title_fullStr | Characterization of carotenoid profiles and presence of functional markers in sub-tropical maize (Zea mays L.) inbred lines |
| title_full_unstemmed | Characterization of carotenoid profiles and presence of functional markers in sub-tropical maize (Zea mays L.) inbred lines |
| title_short | Characterization of carotenoid profiles and presence of functional markers in sub-tropical maize (Zea mays L.) inbred lines |
| title_sort | characterization of carotenoid profiles and presence of functional markers in sub tropical maize zea mays l inbred lines |
| topic | carotenoid kasp provitamin a sub-tropical maize lines zep1 |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-202502-0004_characterization-of-carotenoid-profiles-and-presence-of-functional-markers-in-sub-tropical-maize-zea-mays-l.php |
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