Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging
We have developed, for the Case 3D Cryo-imaging system, a specialized, multiscale visualization scheme which provides color-rich volume rendering and multiplanar reformatting enabling one to visualize an entire mouse and zoom in to organ, tissue, and microscopic scales. With this system, we have ana...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Biomedical Imaging |
| Online Access: | http://dx.doi.org/10.1155/2010/105984 |
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| author | Debashish Roy Madhusudhana Gargesha Grant J. Steyer Parvin Hakimi Richard W. Hanson David L. Wilson |
| author_facet | Debashish Roy Madhusudhana Gargesha Grant J. Steyer Parvin Hakimi Richard W. Hanson David L. Wilson |
| author_sort | Debashish Roy |
| collection | DOAJ |
| description | We have developed, for the Case 3D Cryo-imaging system, a specialized, multiscale visualization scheme which provides color-rich volume rendering and multiplanar reformatting enabling one to visualize an entire mouse and zoom in to organ, tissue, and microscopic scales. With this system, we have anatomically characterized, in 3D, from whole animal to tissue level, a transgenic mouse and compared it with its control. The transgenic mouse overexpresses the cytosolic form of phosphoenolpyruvate carboxykinase (PEPCK-C) in its skeletal muscle and is capable of greatly enhanced physical endurance and has a longer life-span and reproductive life as compared to control animals. We semiautomatically analyzed selected organs such as kidney, heart, adrenal gland, spleen, and ovaries and found comparatively enlarged heart, much less visceral, subcutaneous, and pericardial adipose tissue, and higher tibia-to-femur ratio in the transgenic animal. Microscopically, individual skeletal muscle fibers, fine mesenteric blood vessels, and intestinal villi, among others, were clearly seen. |
| format | Article |
| id | doaj-art-01073c8a906c4d6c91ec891972d7a1b0 |
| institution | DOAJ |
| issn | 1687-4188 1687-4196 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomedical Imaging |
| spelling | doaj-art-01073c8a906c4d6c91ec891972d7a1b02025-08-20T03:22:31ZengWileyInternational Journal of Biomedical Imaging1687-41881687-41962010-01-01201010.1155/2010/105984105984Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-ImagingDebashish Roy0Madhusudhana Gargesha1Grant J. Steyer2Parvin Hakimi3Richard W. Hanson4David L. Wilson5Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USADepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USADepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USADepartment of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USADepartment of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USADepartment of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USAWe have developed, for the Case 3D Cryo-imaging system, a specialized, multiscale visualization scheme which provides color-rich volume rendering and multiplanar reformatting enabling one to visualize an entire mouse and zoom in to organ, tissue, and microscopic scales. With this system, we have anatomically characterized, in 3D, from whole animal to tissue level, a transgenic mouse and compared it with its control. The transgenic mouse overexpresses the cytosolic form of phosphoenolpyruvate carboxykinase (PEPCK-C) in its skeletal muscle and is capable of greatly enhanced physical endurance and has a longer life-span and reproductive life as compared to control animals. We semiautomatically analyzed selected organs such as kidney, heart, adrenal gland, spleen, and ovaries and found comparatively enlarged heart, much less visceral, subcutaneous, and pericardial adipose tissue, and higher tibia-to-femur ratio in the transgenic animal. Microscopically, individual skeletal muscle fibers, fine mesenteric blood vessels, and intestinal villi, among others, were clearly seen.http://dx.doi.org/10.1155/2010/105984 |
| spellingShingle | Debashish Roy Madhusudhana Gargesha Grant J. Steyer Parvin Hakimi Richard W. Hanson David L. Wilson Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging International Journal of Biomedical Imaging |
| title | Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging |
| title_full | Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging |
| title_fullStr | Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging |
| title_full_unstemmed | Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging |
| title_short | Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging |
| title_sort | multi scale characterization of the pepck cmus mouse through 3d cryo imaging |
| url | http://dx.doi.org/10.1155/2010/105984 |
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