Green microalga Chromochloris zofingiensis conserves substrate uptake pattern but changes their metabolic uses across trophic transition
The terrestrial green alga Chromochloris zofingiensis is an emerging model species with potential applications including production of triacylglycerol or astaxanthin. How C. zofingiensis interacts with the diverse substrates during trophic transitions is unknown. To characterize its substrate utiliz...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1470054/full |
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| author | Yuntao Hu Nakian Kim Nakian Kim Melissa S. Roth Katherine B. Louie Katherine B. Louie Suzanne M. Kosina Shivani Upadhyaya Tim L. Jeffers Jacob S. Jordan Benjamin P. Bowen Benjamin P. Bowen Krishna K. Niyogi Krishna K. Niyogi Krishna K. Niyogi Trent R. Northen Trent R. Northen |
| author_facet | Yuntao Hu Nakian Kim Nakian Kim Melissa S. Roth Katherine B. Louie Katherine B. Louie Suzanne M. Kosina Shivani Upadhyaya Tim L. Jeffers Jacob S. Jordan Benjamin P. Bowen Benjamin P. Bowen Krishna K. Niyogi Krishna K. Niyogi Krishna K. Niyogi Trent R. Northen Trent R. Northen |
| author_sort | Yuntao Hu |
| collection | DOAJ |
| description | The terrestrial green alga Chromochloris zofingiensis is an emerging model species with potential applications including production of triacylglycerol or astaxanthin. How C. zofingiensis interacts with the diverse substrates during trophic transitions is unknown. To characterize its substrate utilization and secretion dynamics, we cultivated the alga in a soil-based defined medium in transition between conditions with and without glucose supplementation. Then, we examined its exometabolite and endometabolite profiles. This analysis revealed that regardless of trophic modes, C. zofingiensis preferentially uptakes exogenous lysine, arginine, and purines, while secreting orotic acid. Here, we obtained metabolomic evidences that C. zofingiensis may use arginine for putrescine synthesis when in transition to heterotrophy, and for the TCA cycle during transition to photoautotrophy. We also report that glucose and fructose most effectively inhibited photosynthesis among thirteen different sugars. The utilized or secreted metabolites identified in this study provide important information to improve C. zofingiensis cultivation, and to expand its potential industrial and pharmaceutical applications. |
| format | Article |
| id | doaj-art-00d2ee91c2ab45d6be01dc2528c56087 |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-00d2ee91c2ab45d6be01dc2528c560872025-08-20T02:38:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-11-011510.3389/fmicb.2024.14700541470054Green microalga Chromochloris zofingiensis conserves substrate uptake pattern but changes their metabolic uses across trophic transitionYuntao Hu0Nakian Kim1Nakian Kim2Melissa S. Roth3Katherine B. Louie4Katherine B. Louie5Suzanne M. Kosina6Shivani Upadhyaya7Tim L. Jeffers8Jacob S. Jordan9Benjamin P. Bowen10Benjamin P. Bowen11Krishna K. Niyogi12Krishna K. Niyogi13Krishna K. Niyogi14Trent R. Northen15Trent R. Northen16PrognomiQ Inc., San Mateo, CA, United StatesDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesEnvironmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesEnvironmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesJoint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesEnvironmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, United StatesEnvironmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesJoint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, United StatesHoward Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, United StatesMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesEnvironmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesJoint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesThe terrestrial green alga Chromochloris zofingiensis is an emerging model species with potential applications including production of triacylglycerol or astaxanthin. How C. zofingiensis interacts with the diverse substrates during trophic transitions is unknown. To characterize its substrate utilization and secretion dynamics, we cultivated the alga in a soil-based defined medium in transition between conditions with and without glucose supplementation. Then, we examined its exometabolite and endometabolite profiles. This analysis revealed that regardless of trophic modes, C. zofingiensis preferentially uptakes exogenous lysine, arginine, and purines, while secreting orotic acid. Here, we obtained metabolomic evidences that C. zofingiensis may use arginine for putrescine synthesis when in transition to heterotrophy, and for the TCA cycle during transition to photoautotrophy. We also report that glucose and fructose most effectively inhibited photosynthesis among thirteen different sugars. The utilized or secreted metabolites identified in this study provide important information to improve C. zofingiensis cultivation, and to expand its potential industrial and pharmaceutical applications.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1470054/fullmicroalgaeChromochloris zofingiensisargininepurinemetabolomicsorotic acid |
| spellingShingle | Yuntao Hu Nakian Kim Nakian Kim Melissa S. Roth Katherine B. Louie Katherine B. Louie Suzanne M. Kosina Shivani Upadhyaya Tim L. Jeffers Jacob S. Jordan Benjamin P. Bowen Benjamin P. Bowen Krishna K. Niyogi Krishna K. Niyogi Krishna K. Niyogi Trent R. Northen Trent R. Northen Green microalga Chromochloris zofingiensis conserves substrate uptake pattern but changes their metabolic uses across trophic transition Frontiers in Microbiology microalgae Chromochloris zofingiensis arginine purine metabolomics orotic acid |
| title | Green microalga Chromochloris zofingiensis conserves substrate uptake pattern but changes their metabolic uses across trophic transition |
| title_full | Green microalga Chromochloris zofingiensis conserves substrate uptake pattern but changes their metabolic uses across trophic transition |
| title_fullStr | Green microalga Chromochloris zofingiensis conserves substrate uptake pattern but changes their metabolic uses across trophic transition |
| title_full_unstemmed | Green microalga Chromochloris zofingiensis conserves substrate uptake pattern but changes their metabolic uses across trophic transition |
| title_short | Green microalga Chromochloris zofingiensis conserves substrate uptake pattern but changes their metabolic uses across trophic transition |
| title_sort | green microalga chromochloris zofingiensis conserves substrate uptake pattern but changes their metabolic uses across trophic transition |
| topic | microalgae Chromochloris zofingiensis arginine purine metabolomics orotic acid |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1470054/full |
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