A Strong Impact of Genetic Background on Gut Microflora in Mice
Genetic background affects susceptibility to ileocolitis in mice deficient in two intracellular glutathione peroxidases, GPx1 and GPx2. The C57BL/6 (B6) GPx1/2 double-knockout (DKO) mice have mild ileocolitis, and 129S1/Sv (129) DKO mice have severe inflammation. We used diet to modulate ileocolit...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.4061/2010/986046 |
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| author | R. Steven Esworthy David D. Smith Fong-Fong Chu |
| author_facet | R. Steven Esworthy David D. Smith Fong-Fong Chu |
| author_sort | R. Steven Esworthy |
| collection | DOAJ |
| description | Genetic background affects susceptibility to ileocolitis in mice deficient in two intracellular glutathione peroxidases, GPx1 and GPx2. The C57BL/6 (B6) GPx1/2 double-knockout (DKO) mice have mild ileocolitis, and 129S1/Sv (129) DKO mice have severe inflammation. We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets. Because luminal microbiota induce DKO ileocolitis, we assessed bacterial composition with automated ribosomal intergenic-spacer analysis (ARISA) on cecal DNA. We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes. In comparing AIN and LabDiet, DKO mice were more resistant to change than the non-DKO or WT mice. However, supplementing yeast and inulin to AIN diet greatly altered microflora profiles in the DKO mice. From 129 DKO strictly, we found overgrowth of Escherichia coli. We conclude that genetic background predisposes mice to colonization of potentially pathogenic E. coli. |
| format | Article |
| id | doaj-art-00ca5d863ac64b4fb2a63be36ab6940b |
| institution | OA Journals |
| issn | 2042-0099 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-00ca5d863ac64b4fb2a63be36ab6940b2025-08-20T02:03:13ZengWileyInternational Journal of Inflammation2042-00992010-01-01201010.4061/2010/986046986046A Strong Impact of Genetic Background on Gut Microflora in MiceR. Steven Esworthy0David D. Smith1Fong-Fong Chu2Department of Cancer Biology, Beckman Research Institute of the City of Hope, 1500 Duarte Road, Duarte, CA 91010-3000, USADivision of Information Sciences, Beckman Research Institute of the City of Hope, 1500 Duarte Road, Duarte, CA 91010-3000, USADepartment of Cancer Biology, Beckman Research Institute of the City of Hope, 1500 Duarte Road, Duarte, CA 91010-3000, USAGenetic background affects susceptibility to ileocolitis in mice deficient in two intracellular glutathione peroxidases, GPx1 and GPx2. The C57BL/6 (B6) GPx1/2 double-knockout (DKO) mice have mild ileocolitis, and 129S1/Sv (129) DKO mice have severe inflammation. We used diet to modulate ileocolitis; a casein-based defined diet with AIN76A micronutrients (AIN) attenuates inflammation compared to conventional LabDiets. Because luminal microbiota induce DKO ileocolitis, we assessed bacterial composition with automated ribosomal intergenic-spacer analysis (ARISA) on cecal DNA. We found that mouse strain had the strongest impact on the composition of microbiota than diet and GPx genotypes. In comparing AIN and LabDiet, DKO mice were more resistant to change than the non-DKO or WT mice. However, supplementing yeast and inulin to AIN diet greatly altered microflora profiles in the DKO mice. From 129 DKO strictly, we found overgrowth of Escherichia coli. We conclude that genetic background predisposes mice to colonization of potentially pathogenic E. coli.http://dx.doi.org/10.4061/2010/986046 |
| spellingShingle | R. Steven Esworthy David D. Smith Fong-Fong Chu A Strong Impact of Genetic Background on Gut Microflora in Mice International Journal of Inflammation |
| title | A Strong Impact of Genetic Background on Gut Microflora in Mice |
| title_full | A Strong Impact of Genetic Background on Gut Microflora in Mice |
| title_fullStr | A Strong Impact of Genetic Background on Gut Microflora in Mice |
| title_full_unstemmed | A Strong Impact of Genetic Background on Gut Microflora in Mice |
| title_short | A Strong Impact of Genetic Background on Gut Microflora in Mice |
| title_sort | strong impact of genetic background on gut microflora in mice |
| url | http://dx.doi.org/10.4061/2010/986046 |
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