Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions
Background. This study is aimed at characterizing the human distal airway stem cells (DASCs) and assessing their therapeutic potential in patients with chronic, degenerative lung diseases. These findings will provide a comprehensive understanding for further clinical applications utilizing autologou...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/7123078 |
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| author | Yujia Wang Yi Lu Yingchuan Wu Yufen Sun Yueqing Zhou Qiwang Ma Yawen Zheng Qianwen Yu Yujie Cao Guangdong Chen Ting Zhang Xiaotian Dai Tao Ren Yu Ma Wei Zuo |
| author_facet | Yujia Wang Yi Lu Yingchuan Wu Yufen Sun Yueqing Zhou Qiwang Ma Yawen Zheng Qianwen Yu Yujie Cao Guangdong Chen Ting Zhang Xiaotian Dai Tao Ren Yu Ma Wei Zuo |
| author_sort | Yujia Wang |
| collection | DOAJ |
| description | Background. This study is aimed at characterizing the human distal airway stem cells (DASCs) and assessing their therapeutic potential in patients with chronic, degenerative lung diseases. These findings will provide a comprehensive understanding for further clinical applications utilizing autologous airway stem cells as therapeutic intervention in respiratory diseases. Methods. DASCs were isolated from healthy subjects or patients diagnosed with bronchiectasis, chronic obstructive pulmonary diseases (COPD), or interstitial lung disease (ILD). Differentiation capacity, a key property of the stem cells, was studied using a novel monolayer differentiation system. The differentiated cells were evaluated for alveolar and bronchial cell marker expression, and the quantified expression level of differentiated cells was further examined for their relationship with age and pulmonary function of the subjects. Results and Conclusions. Differentiation of DASCs and tracheal stem cells (TSCs) yielded an alveolus-like structure and a tube-shaped structure, respectively, with distinct marker gene expression. Additionally, single-cell-derived clones showed diverse differentiation fates, even if the clones arise from identical or different individuals. More importantly, the alveolar differentiation potency was higher in DASCs derived from patients than from healthy people. The differentiation efficiency of DASCs also correlates with age in patients with bronchiectasis and ILD. |
| format | Article |
| id | doaj-art-00ba9e2fb02c42c59f77ede5dae01d59 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-00ba9e2fb02c42c59f77ede5dae01d592025-08-20T02:03:13ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/71230787123078Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological ConditionsYujia Wang0Yi Lu1Yingchuan Wu2Yufen Sun3Yueqing Zhou4Qiwang Ma5Yawen Zheng6Qianwen Yu7Yujie Cao8Guangdong Chen9Ting Zhang10Xiaotian Dai11Tao Ren12Yu Ma13Wei Zuo14East Hospital, School of Medicine, Tongji University, Shanghai 200120, ChinaShanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, ChinaEast Hospital, School of Medicine, Tongji University, Shanghai 200120, ChinaEast Hospital, School of Medicine, Tongji University, Shanghai 200120, ChinaEast Hospital, School of Medicine, Tongji University, Shanghai 200120, ChinaEast Hospital, School of Medicine, Tongji University, Shanghai 200120, ChinaKiangnan Stem Cell Institute, Zhejiang 311300, ChinaKiangnan Stem Cell Institute, Zhejiang 311300, ChinaKiangnan Stem Cell Institute, Zhejiang 311300, ChinaKiangnan Stem Cell Institute, Zhejiang 311300, ChinaKiangnan Stem Cell Institute, Zhejiang 311300, ChinaSouthwest Hospital, Third Military Medical University of PLA, Chongqing 400038, ChinaShanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, ChinaEast Hospital, School of Medicine, Tongji University, Shanghai 200120, ChinaEast Hospital, School of Medicine, Tongji University, Shanghai 200120, ChinaBackground. This study is aimed at characterizing the human distal airway stem cells (DASCs) and assessing their therapeutic potential in patients with chronic, degenerative lung diseases. These findings will provide a comprehensive understanding for further clinical applications utilizing autologous airway stem cells as therapeutic intervention in respiratory diseases. Methods. DASCs were isolated from healthy subjects or patients diagnosed with bronchiectasis, chronic obstructive pulmonary diseases (COPD), or interstitial lung disease (ILD). Differentiation capacity, a key property of the stem cells, was studied using a novel monolayer differentiation system. The differentiated cells were evaluated for alveolar and bronchial cell marker expression, and the quantified expression level of differentiated cells was further examined for their relationship with age and pulmonary function of the subjects. Results and Conclusions. Differentiation of DASCs and tracheal stem cells (TSCs) yielded an alveolus-like structure and a tube-shaped structure, respectively, with distinct marker gene expression. Additionally, single-cell-derived clones showed diverse differentiation fates, even if the clones arise from identical or different individuals. More importantly, the alveolar differentiation potency was higher in DASCs derived from patients than from healthy people. The differentiation efficiency of DASCs also correlates with age in patients with bronchiectasis and ILD.http://dx.doi.org/10.1155/2019/7123078 |
| spellingShingle | Yujia Wang Yi Lu Yingchuan Wu Yufen Sun Yueqing Zhou Qiwang Ma Yawen Zheng Qianwen Yu Yujie Cao Guangdong Chen Ting Zhang Xiaotian Dai Tao Ren Yu Ma Wei Zuo Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions Stem Cells International |
| title | Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions |
| title_full | Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions |
| title_fullStr | Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions |
| title_full_unstemmed | Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions |
| title_short | Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions |
| title_sort | alveolar differentiation potency of human distal airway stem cells is associated with pulmonary pathological conditions |
| url | http://dx.doi.org/10.1155/2019/7123078 |
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