Impact of allogeneic dental pulp stem cell injection on tissue regeneration in periodontitis: a multicenter randomized clinical trial
Abstract Periodontitis causes the destruction of tooth-supporting tissues, and current therapies for periodontal regeneration are invasive. In this study, a human dental pulp stem cell (DPSC; hDP-MSC) injection was developed to promote periodontal regeneration through a non-invasive procedure. A tot...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-07-01
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| Series: | Signal Transduction and Targeted Therapy |
| Online Access: | https://doi.org/10.1038/s41392-025-02320-w |
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| author | Yi Liu Yitong Liu Jingchao Hu Jianhui Han Lin Song Xu Liu Nannan Han Xia Xia Jia He Hongfang Meng Meng Wan Haojie Wang Xiaodan Liu Zhanyi Gao Xiao Wang Chutse Wu Songlin Wang |
| author_facet | Yi Liu Yitong Liu Jingchao Hu Jianhui Han Lin Song Xu Liu Nannan Han Xia Xia Jia He Hongfang Meng Meng Wan Haojie Wang Xiaodan Liu Zhanyi Gao Xiao Wang Chutse Wu Songlin Wang |
| author_sort | Yi Liu |
| collection | DOAJ |
| description | Abstract Periodontitis causes the destruction of tooth-supporting tissues, and current therapies for periodontal regeneration are invasive. In this study, a human dental pulp stem cell (DPSC; hDP-MSC) injection was developed to promote periodontal regeneration through a non-invasive procedure. A total of 132 patients with chronic periodontitis (158 teeth) from two centers in China were included. Thirty-six were randomly assigned to different DPSC dose groups (ranging from 1 × 106 to 1 × 107 DPSCs per tooth, with nine injected with saline only), and 96 were randomly assigned to a single-injection group (1 × 107/0.6 mL DPSCs), a double-injection group (1 × 107/0.6 mL DPSCs × 2), or a saline group, in a 1:1:1 ratio. At 6 months post-therapy, attachment loss (AL), periodontal probing depth (PD), gingival recession (GR), tooth mobility (TM), and bone defect depth (BDD) were examined. The primary outcome was AL. DPSC injection resulted in greater improvement in BDD (0.30 ± 0.484 mm) compared to saline injection (0.04 ± 0.315 mm). Post hoc analysis showed that DPSC injection had significantly better outcomes in patients with stage III periodontitis (AL ≥ 5 mm): 54 patients received DPSCs, and 40 received saline. AL improved by 1.67 ± 1.508 mm in the DPSC group (26.81% improvement) and by 1.03 ± 1.310 mm in the saline group (17.43% improvement). The therapeutic effects encompassed improvements in both soft and hard tissues. In summary, DPSC injection was safe and improved clinical outcomes compared to saline injection in patients with stage III periodontitis. Larger trials are warranted to validate these findings (ClinicalTrials.gov registration: NCT05924373). |
| format | Article |
| id | doaj-art-00a66585a15a479699985007cdff4ee6 |
| institution | Kabale University |
| issn | 2059-3635 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Signal Transduction and Targeted Therapy |
| spelling | doaj-art-00a66585a15a479699985007cdff4ee62025-08-20T03:41:57ZengNature Publishing GroupSignal Transduction and Targeted Therapy2059-36352025-07-0110111110.1038/s41392-025-02320-wImpact of allogeneic dental pulp stem cell injection on tissue regeneration in periodontitis: a multicenter randomized clinical trialYi Liu0Yitong Liu1Jingchao Hu2Jianhui Han3Lin Song4Xu Liu5Nannan Han6Xia Xia7Jia He8Hongfang Meng9Meng Wan10Haojie Wang11Xiaodan Liu12Zhanyi Gao13Xiao Wang14Chutse Wu15Songlin Wang16Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical UniversityLaboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical UniversityLaboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical UniversityDepartment of Radiology, School of Stomatology, Capital Medical UniversityLaboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical UniversityLaboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical UniversityLaboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical UniversityBeijing SH Biotechnology Co. Ltd.Beijing SH Biotechnology Co. Ltd.Beijing SH Biotechnology Co. Ltd.Department of Stomatology, Peking University Third HospitalDepartment of Stomatology, Peking University Third HospitalDepartment of Stomatology, Peking University Third HospitalDepartment of Stomatology, Peking University Third HospitalDepartment of Stomatology, Peking University Third HospitalBeijing SH Biotechnology Co. Ltd.Beijing Laboratory of Oral Health, Capital Medical UniversityAbstract Periodontitis causes the destruction of tooth-supporting tissues, and current therapies for periodontal regeneration are invasive. In this study, a human dental pulp stem cell (DPSC; hDP-MSC) injection was developed to promote periodontal regeneration through a non-invasive procedure. A total of 132 patients with chronic periodontitis (158 teeth) from two centers in China were included. Thirty-six were randomly assigned to different DPSC dose groups (ranging from 1 × 106 to 1 × 107 DPSCs per tooth, with nine injected with saline only), and 96 were randomly assigned to a single-injection group (1 × 107/0.6 mL DPSCs), a double-injection group (1 × 107/0.6 mL DPSCs × 2), or a saline group, in a 1:1:1 ratio. At 6 months post-therapy, attachment loss (AL), periodontal probing depth (PD), gingival recession (GR), tooth mobility (TM), and bone defect depth (BDD) were examined. The primary outcome was AL. DPSC injection resulted in greater improvement in BDD (0.30 ± 0.484 mm) compared to saline injection (0.04 ± 0.315 mm). Post hoc analysis showed that DPSC injection had significantly better outcomes in patients with stage III periodontitis (AL ≥ 5 mm): 54 patients received DPSCs, and 40 received saline. AL improved by 1.67 ± 1.508 mm in the DPSC group (26.81% improvement) and by 1.03 ± 1.310 mm in the saline group (17.43% improvement). The therapeutic effects encompassed improvements in both soft and hard tissues. In summary, DPSC injection was safe and improved clinical outcomes compared to saline injection in patients with stage III periodontitis. Larger trials are warranted to validate these findings (ClinicalTrials.gov registration: NCT05924373).https://doi.org/10.1038/s41392-025-02320-w |
| spellingShingle | Yi Liu Yitong Liu Jingchao Hu Jianhui Han Lin Song Xu Liu Nannan Han Xia Xia Jia He Hongfang Meng Meng Wan Haojie Wang Xiaodan Liu Zhanyi Gao Xiao Wang Chutse Wu Songlin Wang Impact of allogeneic dental pulp stem cell injection on tissue regeneration in periodontitis: a multicenter randomized clinical trial Signal Transduction and Targeted Therapy |
| title | Impact of allogeneic dental pulp stem cell injection on tissue regeneration in periodontitis: a multicenter randomized clinical trial |
| title_full | Impact of allogeneic dental pulp stem cell injection on tissue regeneration in periodontitis: a multicenter randomized clinical trial |
| title_fullStr | Impact of allogeneic dental pulp stem cell injection on tissue regeneration in periodontitis: a multicenter randomized clinical trial |
| title_full_unstemmed | Impact of allogeneic dental pulp stem cell injection on tissue regeneration in periodontitis: a multicenter randomized clinical trial |
| title_short | Impact of allogeneic dental pulp stem cell injection on tissue regeneration in periodontitis: a multicenter randomized clinical trial |
| title_sort | impact of allogeneic dental pulp stem cell injection on tissue regeneration in periodontitis a multicenter randomized clinical trial |
| url | https://doi.org/10.1038/s41392-025-02320-w |
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